Fungi

Density Gradient Centrifugation for Enrichment and Identification of GFP-tagged Chitosomal Microvesicles of Filamentous Fungi

Authors: Jorge Verdín*
Jorge VerdínAffiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Mexico
Present address: Industrial Biotechnology, Jalisco State Research and Technology Assistance Center (CIATEJ), Guadalajara, Mexico
Bio-protocol author page: a2615
, Eddy Sánchez-León*
Eddy Sánchez-LeónAffiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Mexico
Bio-protocol author page: a2616
, Rosa Fajardo-Somera
Rosa Fajardo-SomeraAffiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Mexico
Bio-protocol author page: a2617
, Carlos A. Leal Morales
Carlos A. Leal MoralesAffiliation: Department of Biology, Campus Guanajuato, University of Guanajuato, Guanajuato, Mexico
Bio-protocol author page: a2618
, Salomón Bartnicki-García
Salomón Bartnicki-GarcíaAffiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Mexico
Bio-protocol author page: a2619
and Meritxell Riquelme
Meritxell RiquelmeAffiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Mexico
For correspondence: riquelme@cicese.mx
Bio-protocol author page: a2620
(*contributed equally to this work), date: 10/5/2015, 3427 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1611.

[Abstract] Density gradient centrifugation has been utilized to characterize the subcellular distribution of physiologically relevant enzymes in yeasts and filamentous fungi (Leal-Morales et al., 1988; Martínez et al., 1989; Kamada et al., 1991). This approach is now potentiated by protein tagging and live imaging ...

Density Gradient Centrifugation for Enrichment and Identification of GFP-tagged Chitosomal Microvesicles of Filamentous Fungi

Authors: Jorge Verdín*
Jorge VerdínAffiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Mexico
Present address: Industrial Biotechnology, Jalisco State Research and Technology Assistance Center (CIATEJ), Guadalajara, Mexico
Bio-protocol author page: a2615
, Eddy Sánchez-León*
Eddy Sánchez-LeónAffiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Mexico
Bio-protocol author page: a2616
, Rosa Fajardo-Somera
Rosa Fajardo-SomeraAffiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Mexico
Bio-protocol author page: a2617
, Carlos A. Leal Morales
Carlos A. Leal MoralesAffiliation: Department of Biology, Campus Guanajuato, University of Guanajuato, Guanajuato, Mexico
Bio-protocol author page: a2618
, Salomón Bartnicki-García
Salomón Bartnicki-GarcíaAffiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Mexico
Bio-protocol author page: a2619
and Meritxell Riquelme
Meritxell RiquelmeAffiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Mexico
For correspondence: riquelme@cicese.mx
Bio-protocol author page: a2620
(*contributed equally to this work), date: 10/5/2015, 3427 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1611.

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