Fungi

A Reliable Assay to Evaluate the Virulence of Aspergillus nidulans Using the Alternative Animal Model Galleria mellonella (Lepidoptera)

Authors: Caroline Mota Fernandes
Caroline Mota FernandesAffiliation: Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
For correspondence: cmota@biof.ufrj.br
Bio-protocol author page: a4626
, Fernanda Lopes Fonseca
Fernanda Lopes FonsecaAffiliation: Centro de Desenvolvimento Tecnológico em Saúde, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil
Bio-protocol author page: a4627
, Gustavo Henrique Goldman
Gustavo Henrique GoldmanAffiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil
Bio-protocol author page: a4628
, Marcos Dias Pereira*
Marcos Dias PereiraAffiliation: Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
For correspondence: marcosdp@iq.ufrj.br
Bio-protocol author page: a4629
and Eleonora Kurtenbach*
Eleonora KurtenbachAffiliation: Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
Bio-protocol author page: a4630
(*contributed equally to this work), date: 6/5/2017, 284 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2329.

[Abstract] The greater wax moth Galleria mellonella has emerged as an effective heterologous host to study fungal pathogenesis and the efficacy of promising antifungal drugs (Mylonakis et al., 2005; Li et al., 2013). Here, a methodology describing the Aspergillus nidulans infection in G. mellonella larvae, along ...

XTT Assay of Antifungal Activity

Authors: Flávio V. Loures
Flávio V. LouresAffiliation 1: Department of Medicine, University of Massachusetts Medical School, Massachusetts, United States of America
Affiliation 2: Department of Immunology, Institute of Biomedical Sciences, São Paulo University, São Paulo, Brazil
For correspondence: loures@icb.usp.br
Bio-protocol author page: a2409
and Stuart M. Levitz
Stuart M. LevitzAffiliation: Department of Medicine, University of Massachusetts Medical School, Massachusetts, USA
Bio-protocol author page: a2410
, date: 8/5/2015, 2428 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1543.

[Abstract] XTT assay is a colorimetric method that uses the tetrazolium dye, 2,3-bis-(2-methoxy-4-nitro-5-sulphenyl)-(2H)-tetrazolium-5-carboxanilide (XTT) to quantify cell-mediated damage to fungi. Actively respiring fungal cells convert the water-soluble XTT to a water-soluble, orange colored formazan product ...

XTT Assay of Antifungal Activity

Authors: Flávio V. Loures
Flávio V. LouresAffiliation 1: Department of Medicine, University of Massachusetts Medical School, Massachusetts, United States of America
Affiliation 2: Department of Immunology, Institute of Biomedical Sciences, São Paulo University, São Paulo, Brazil
For correspondence: loures@icb.usp.br
Bio-protocol author page: a2409
and Stuart M. Levitz
Stuart M. LevitzAffiliation: Department of Medicine, University of Massachusetts Medical School, Massachusetts, USA
Bio-protocol author page: a2410
, date: 8/5/2015, 2428 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1543.

Fungi

Alternaria

Aspergillus

Aspergillus fumigatus

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