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Genetic Transformation of
Candida glabrata
by Heat Shock
Authors:
Fabian Istel*
Fabian Istel
Affiliation:
Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University Vienna, Vienna, Austria
Bio-protocol author page:
a2372
,
Tobias Schwarzmüller*
Tobias Schwarzmüller
Affiliation:
Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University Vienna, Vienna, Austria
Bio-protocol author page:
a2373
,
Michael Tscherner
Michael Tscherner
Affiliation:
Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University Vienna, Vienna, Austria
Bio-protocol author page:
a929
and
Karl Kuchler
Karl Kuchler
Affiliation:
Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University Vienna, Vienna, Austria
For correspondence:
karl.kuchler@meduniwien.ac.at
Bio-protocol author page:
a496
(*contributed equally to this work)
,
date: 7/20/2015, 2355 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1529
.
[Abstract] Here, we report a method for the transformation of
Candida glabrata
using a heat shock method. The protocol can be used for transformations in single well or in 96-well scale. It has been employed as an alternative method to the electroporation protocol to construct a genome-scale gene deletion collection ...
Genetic Transformation of
Candida glabrata
by Electroporation
Authors:
Fabian Istel*
Fabian Istel
Affiliation:
Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University Vienna, Vienna, Austria
Bio-protocol author page:
a2372
,
Tobias Schwarzmüller*
Tobias Schwarzmüller
Affiliation:
Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University Vienna, Vienna, Austria
Bio-protocol author page:
a2373
,
Michael Tscherner
Michael Tscherner
Affiliation:
Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University Vienna, Vienna, Austria
Bio-protocol author page:
a929
and
Karl Kuchler
Karl Kuchler
Affiliation:
Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University Vienna, Vienna, Austria
For correspondence:
karl.kuchler@meduniwien.ac.at
Bio-protocol author page:
a496
(*contributed equally to this work)
,
date: 7/20/2015, 2157 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1528
.
[Abstract] Here, we report a method for the transformation by electroporation of the human fungal pathogen
Candida glabrata
(
C. glabrata
). The protocol can be used for transformations in single well or in 96-well microtiter plates. It has been extensively used to generate a genome-scale gene deletion library using ...
1
Genetic Transformation of
Candida glabrata
by Heat Shock
Authors:
Fabian Istel*
Fabian Istel
Affiliation:
Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University Vienna, Vienna, Austria
Bio-protocol author page:
a2372
,
Tobias Schwarzmüller*
Tobias Schwarzmüller
Affiliation:
Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University Vienna, Vienna, Austria
Bio-protocol author page:
a2373
,
Michael Tscherner
Michael Tscherner
Affiliation:
Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University Vienna, Vienna, Austria
Bio-protocol author page:
a929
and
Karl Kuchler
Karl Kuchler
Affiliation:
Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University Vienna, Vienna, Austria
For correspondence:
karl.kuchler@meduniwien.ac.at
Bio-protocol author page:
a496
(*contributed equally to this work)
,
date: 7/20/2015, 2355 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1529
.
[Abstract] Here, we report a method for the transformation of
Candida glabrata
using a heat shock method. The protocol can be used for transformations in single well or in 96-well scale. It has been employed as an alternative method to the electroporation protocol to construct a genome-scale gene deletion collection ...
Genetic Transformation of
Candida glabrata
by Electroporation
Authors:
Fabian Istel*
Fabian Istel
Affiliation:
Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University Vienna, Vienna, Austria
Bio-protocol author page:
a2372
,
Tobias Schwarzmüller*
Tobias Schwarzmüller
Affiliation:
Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University Vienna, Vienna, Austria
Bio-protocol author page:
a2373
,
Michael Tscherner
Michael Tscherner
Affiliation:
Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University Vienna, Vienna, Austria
Bio-protocol author page:
a929
and
Karl Kuchler
Karl Kuchler
Affiliation:
Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University Vienna, Vienna, Austria
For correspondence:
karl.kuchler@meduniwien.ac.at
Bio-protocol author page:
a496
(*contributed equally to this work)
,
date: 7/20/2015, 2157 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1528
.
[Abstract] Here, we report a method for the transformation by electroporation of the human fungal pathogen
Candida glabrata
(
C. glabrata
). The protocol can be used for transformations in single well or in 96-well microtiter plates. It has been extensively used to generate a genome-scale gene deletion library using ...
1
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