Mammalia

FICZ Exposure and Viral Infection in Mice

Authors: Taisho Yamada
Taisho YamadaAffiliation: Division of Signaling in Cancer and Immunology, Institute for Genetic Medicine, Molecular Medical Biochemistry Unit, Biological Chemistry and Engineering Course, Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo, Japan
Bio-protocol author page: a3976
and Akinori Takaoka
Akinori TakaokaAffiliation: Division of Signaling in Cancer and Immunology, Institute for Genetic Medicine, Molecular Medical Biochemistry Unit, Biological Chemistry and Engineering Course, Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo, Japan
For correspondence: takaoka@igm.hokudai.ac.jp
Bio-protocol author page: a2879
, date: 1/5/2017, 930 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2096.

[Abstract] The aryl hydrocarbon receptor (AHR) is known as a sensor for dioxins that mediates their toxicity, and also has important biophysiological roles such as circadian rhythms, cell differentiation and immune responses. 6-formylindolo(3,2-b)carbazole (FICZ), which is derived through the metabolism of L-tryptophan ...

Mouse Models of Uncomplicated and Fatal Malaria

Authors: Brian W. Huang*
Brian W. HuangAffiliation: Division of Experimental Medicine, Department of Medicine, University of California, San Francisco, San Francisco, USA
Bio-protocol author page: a2331
, Emily Pearman*
Emily PearmanAffiliation: Division of Experimental Medicine, Department of Medicine, University of California, San Francisco, San Francisco, USA
Bio-protocol author page: a2332
and Charles C. Kim
Charles C. KimAffiliation: Division of Experimental Medicine, Department of Medicine, University of California, San Francisco, San Francisco, USA
For correspondence: charlie.kim@ucsf.edu
Bio-protocol author page: a2333
(*contributed equally to this work), date: 7/5/2015, 3972 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1514.

[Abstract] Mouse models have demonstrated utility in delineating the mechanisms underlying many aspects of malaria immunology and physiology. The most common mouse models of malaria employ the rodent-specific parasite species Plasmodium berghei, P. yoelii, and P. chabaudi, which elicit distinct pathologies and ...

	In vitro Culture of Human PBMCs
	Detection of Intracellular Reactive Oxygen Species (CM-H2DCFDA)
	Analysis of Protein Stability by the Cycloheximide Chase Assay
	Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation
	Dimethylmethylene Blue Assay (DMMB)
	Isolation of Human PBMCs
	BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry
	Analysis of Intestinal Permeability in Mice
	Isolation of Mouse Tumor-Infiltrating Leukocytes by Percoll Gradient Centrifugation
	Subcellular Fractionation of Cultured Human Cell Lines