Bacteria

Activity Assays for Bacteriophage Endolysin PlyPy

Authors: Rolf Lood
Rolf LoodAffiliation: Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, New York, USA
Bio-protocol author page: a1648
and Vincent A. Fischetti
Vincent A. FischettiAffiliation: Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, New York, USA
For correspondence: vaf@rockefeller.edu
Bio-protocol author page: a1649
, date: 9/20/2014, 2315 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1233.

[Abstract] Bacterial viruses (bacteriophages) escape and kill their host by degrading the bacterial peptidoglycan layer through the mechanism of enzymes called endolysins: peptidoglycan degrading enzymes. The method included here is useful for the initial characterization of any endolysin, regardless of the specific ...

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