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Aorta Ring Assay

Authors: Jing Jin
Jing JinAffiliation 1: Second Affiliated hospital Zhejiang University college of Medicine of clinic research center, Hangzhou, China
Affiliation 2: Cardiovascular key laboratory of Zhejiang province, Hangzhou, China
Bio-protocol author page: a3272
, Xinyang Hu
Xinyang HuAffiliation 1: Second Affiliated hospital Zhejiang University college of Medicine of clinic research center, Hangzhou, China
Affiliation 2: Second Affiliated hospital Zhejiang University college of Medicine of Department of science and education, Hangzhou, China
Bio-protocol author page: a3273
, Ling Zhang
Ling ZhangAffiliation: Cardiovascular key laboratory of Zhejiang province, Hangzhou, China
Bio-protocol author page: a3367
 and Jian'an Wang
Jian'an WangAffiliation 1: Second Affiliated hospital Zhejiang University college of Medicine of clinic research center, Hangzhou, China
Affiliation 2: Cardiovascular key laboratory of Zhejiang province, Hangzhou, China
For correspondence: jian_an_wang@yahoo.com
Bio-protocol author page: a3368
,  date: 7/5/2016, 1537 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1856.

[Abstract] Angiogenesis is the nature and the pathological process of blood vessels growth from pre-existing vascular bud. It plays an important role in cancer and cardiovascular disease. Aorta Ring Assay is an approach of Angiogenesis. In this experiment, we use the aorta of rat as material, clean surrounding ...

Transfection of Embryoid Bodies with miRNA Precursors to Induce Cardiac Differentiation

Authors: Federica Pisano
Federica PisanoAffiliation 1: Department of Cardiothoracic and Vascular Sciences-Coronary Care Unit and Laboratory of Clinical and Experimental Cardiology-Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
Affiliation 2: Laboratory of Experimental Cardiology for Cell and Molecular Therapy, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
Bio-protocol author page: a2897
 and Massimiliano Gnecchi
Massimiliano GnecchiAffiliation 1: Department of Cardiothoracic and Vascular Sciences-Coronary Care Unit and Laboratory of Clinical and Experimental Cardiology-Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
Affiliation 2: Laboratory of Experimental Cardiology for Cell and Molecular Therapy, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
Affiliation 3: Department of Molecular Medicine-Unit of Cardiology-University of Pavia, Pavia, Italy
Affiliation 4: Department of Medicine-University of Cape Town-Cape Town, South Africa
For correspondence: m.gnecchi@unipv.it
Bio-protocol author page: a2898
,  date: 2/5/2016, 1672 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1726.

[Abstract] In recent years, the utilization of stem cell therapy to regenerate cardiac tissue has been proposed as a possible strategy to treat cardiac damage (Gnecchi et al., 2012, Aguirre et al., 2013; Sanganalmath and Bolli, 2013). Although encouraging results have been obtained in experimental models, the ...

Permanent Occlusion of the Left Anterior Coronary Artery in the Rat

Authors: Mélanie Bousquenaud
Mélanie BousquenaudAffiliation: Laboratory of Pathology, Dept of Medicine, University of Fribourg, Fribourg, Switzerland
For correspondence: melanie.bousquenaud@unifr.ch
Bio-protocol author page: a2737
, Pierre-Yves Marie
Pierre-Yves MarieAffiliation: Nancyclotep, Experimental Imaging Platform, Nancy, France
Bio-protocol author page: a2738
 and Yvan Devaux
Yvan DevauxAffiliation: Laboratory of Cardiovascular Research, Luxembourg Institut of Health, Luxembourg, Luxembourg
Bio-protocol author page: a2642
,  date: 11/20/2015, 1366 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1663.

[Abstract] Left ventricular (LV) remodeling occurs in many patients after myocardial infarction (MI). LV remodeling is characterized by progressive ventricular dilatation and contractile dysfunction, consequently to cardiomyocyte hypertrophy and fibrosis. Despite reperfusion therapies, this pathophysiological ...

Rat Aortic Ring Model to Assay Angiogenesis ex vivo

Authors: Isabelle Ernens
Isabelle ErnensAffiliation: Laboratory of Cardiovascular Research, Luxembourg Institute of Health (L.I.H.), Luxembourg, Luxembourg
For correspondence: isabelle.ernens@lih.lu
Bio-protocol author page: a2640
, Bénédicte Lenoir
Bénédicte LenoirAffiliation: Laboratory of Cardiovascular Research, Luxembourg Institute of Health (L.I.H.), Luxembourg, Luxembourg
Bio-protocol author page: a2641
, Yvan Devaux
Yvan DevauxAffiliation: Laboratory of Cardiovascular Research, Luxembourg Institute of Health (L.I.H.), Luxembourg, Luxembourg
Bio-protocol author page: a2642
 and Daniel R. Wagner
Daniel R. WagnerAffiliation: Division of Cardiology, Centre Hospitalier, Luxembourg, Luxembourg
Bio-protocol author page: a2643
,  date: 10/20/2015, 2152 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1622.

[Abstract] Angiogenesis is a multifactorial event which requires the migration, proliferation, differentiation and structure rearrangement of endothelial cells. This angiogenic process has been commonly studied using in vitro assays such as Boyden chamber assay, wound healing assay and tube formation assay. These ...

Monocular Deprivation in Mice

Authors: Ya-tang Li
Ya-tang LiAffiliation: Zilkha Neurogenetic Institute, University of Southern California, Los Angeles, USA
For correspondence: yatangli8@gmail.com
Bio-protocol author page: a1109
, Xiao-lin Chou
Xiao-lin ChouAffiliation: Zilkha Neurogenetic Institute, University of Southern California, Los Angeles, USA
Bio-protocol author page: a1131
 and Huizhong Whit Tao
Huizhong Whit Tao Affiliation: Zilkha Neurogenetic Institute, University of Southern California, Los Angeles, USA
Bio-protocol author page: a1132
,  date: 1/20/2014, 3296 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1024.

[Abstract] Monocular deprivation is an experimental technique to study the ocular dominance plasticity during critical period (Hubel and Wiesel, 1963). Generally one eye of an animal is sutured during critical period, and the sutured eye is re-opened after either less than three days (short term) or more than ...

Assay of Blood Brain Barrier and Placental Barrier Permeability

Authors: Saurabh Kumar Agnihotri*
Saurabh Kumar AgnihotriAffiliation: Endocrinology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Bio-protocol author page: a726
, Poonam Singh*
Poonam SinghAffiliation: Toxicology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Bio-protocol author page: a727
, Balawant Kumar
Balawant KumarAffiliation: Toxicology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Bio-protocol author page: a728
, Pankaj Singh
Pankaj SinghAffiliation: Endocrinology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Bio-protocol author page: a729
, Swatantra Kumar Jain
Swatantra Kumar JainAffiliation: Endocrinology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Bio-protocol author page: a730
, Mahesh Chandra Tewari
Mahesh Chandra TewariAffiliation: Endocrinology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Bio-protocol author page: a731
, Sadan Kumar
Sadan KumarAffiliation: Toxicology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Bio-protocol author page: a732
, Monika Sachdev
Monika SachdevAffiliation: Endocrinology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
For correspondence: smonika26@yahoo.com
Bio-protocol author page: a400
 and Raj Kamal Tripathi
Raj Kamal TripathiAffiliation: Toxicology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
For correspondence: rk_tripathi@cdri.res.in
Bio-protocol author page: a734
 (*contributed equally to this work),  date: 8/5/2013, 3955 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.845.

[Abstract] Evans blue dye solution was used to observe the effect of a viral protein on two pre-defined barriers of the body i.e. the blood brain barrier (BBB) and the placental barrier (PB). This dye has strong affinity for serum albumin and does not cross these barriers under natural conditions. As all the dye ...
1 

Assay of Blood Brain Barrier and Placental Barrier Permeability

Authors: Saurabh Kumar Agnihotri*
Saurabh Kumar AgnihotriAffiliation: Endocrinology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Bio-protocol author page: a726
, Poonam Singh*
Poonam SinghAffiliation: Toxicology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Bio-protocol author page: a727
, Balawant Kumar
Balawant KumarAffiliation: Toxicology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Bio-protocol author page: a728
, Pankaj Singh
Pankaj SinghAffiliation: Endocrinology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Bio-protocol author page: a729
, Swatantra Kumar Jain
Swatantra Kumar JainAffiliation: Endocrinology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Bio-protocol author page: a730
, Mahesh Chandra Tewari
Mahesh Chandra TewariAffiliation: Endocrinology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Bio-protocol author page: a731
, Sadan Kumar
Sadan KumarAffiliation: Toxicology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Bio-protocol author page: a732
, Monika Sachdev
Monika SachdevAffiliation: Endocrinology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
For correspondence: smonika26@yahoo.com
Bio-protocol author page: a400
 and Raj Kamal Tripathi
Raj Kamal TripathiAffiliation: Toxicology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
For correspondence: rk_tripathi@cdri.res.in
Bio-protocol author page: a734
 (*contributed equally to this work),  date: 8/5/2013, 3955 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.845.

[Abstract] Evans blue dye solution was used to observe the effect of a viral protein on two pre-defined barriers of the body i.e. the blood brain barrier (BBB) and the placental barrier (PB). This dye has strong affinity for serum albumin and does not cross these barriers under natural conditions. As all the dye ...

Monocular Deprivation in Mice

Authors: Ya-tang Li
Ya-tang LiAffiliation: Zilkha Neurogenetic Institute, University of Southern California, Los Angeles, USA
For correspondence: yatangli8@gmail.com
Bio-protocol author page: a1109
, Xiao-lin Chou
Xiao-lin ChouAffiliation: Zilkha Neurogenetic Institute, University of Southern California, Los Angeles, USA
Bio-protocol author page: a1131
 and Huizhong Whit Tao
Huizhong Whit Tao Affiliation: Zilkha Neurogenetic Institute, University of Southern California, Los Angeles, USA
Bio-protocol author page: a1132
,  date: 1/20/2014, 3296 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1024.

[Abstract] Monocular deprivation is an experimental technique to study the ocular dominance plasticity during critical period (Hubel and Wiesel, 1963). Generally one eye of an animal is sutured during critical period, and the sutured eye is re-opened after either less than three days (short term) or more than ...

Rat Aortic Ring Model to Assay Angiogenesis ex vivo

Authors: Isabelle Ernens
Isabelle ErnensAffiliation: Laboratory of Cardiovascular Research, Luxembourg Institute of Health (L.I.H.), Luxembourg, Luxembourg
For correspondence: isabelle.ernens@lih.lu
Bio-protocol author page: a2640
, Bénédicte Lenoir
Bénédicte LenoirAffiliation: Laboratory of Cardiovascular Research, Luxembourg Institute of Health (L.I.H.), Luxembourg, Luxembourg
Bio-protocol author page: a2641
, Yvan Devaux
Yvan DevauxAffiliation: Laboratory of Cardiovascular Research, Luxembourg Institute of Health (L.I.H.), Luxembourg, Luxembourg
Bio-protocol author page: a2642
 and Daniel R. Wagner
Daniel R. WagnerAffiliation: Division of Cardiology, Centre Hospitalier, Luxembourg, Luxembourg
Bio-protocol author page: a2643
,  date: 10/20/2015, 2152 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1622.

[Abstract] Angiogenesis is a multifactorial event which requires the migration, proliferation, differentiation and structure rearrangement of endothelial cells. This angiogenic process has been commonly studied using in vitro assays such as Boyden chamber assay, wound healing assay and tube formation assay. These ...

Transfection of Embryoid Bodies with miRNA Precursors to Induce Cardiac Differentiation

Authors: Federica Pisano
Federica PisanoAffiliation 1: Department of Cardiothoracic and Vascular Sciences-Coronary Care Unit and Laboratory of Clinical and Experimental Cardiology-Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
Affiliation 2: Laboratory of Experimental Cardiology for Cell and Molecular Therapy, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
Bio-protocol author page: a2897
 and Massimiliano Gnecchi
Massimiliano GnecchiAffiliation 1: Department of Cardiothoracic and Vascular Sciences-Coronary Care Unit and Laboratory of Clinical and Experimental Cardiology-Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
Affiliation 2: Laboratory of Experimental Cardiology for Cell and Molecular Therapy, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
Affiliation 3: Department of Molecular Medicine-Unit of Cardiology-University of Pavia, Pavia, Italy
Affiliation 4: Department of Medicine-University of Cape Town-Cape Town, South Africa
For correspondence: m.gnecchi@unipv.it
Bio-protocol author page: a2898
,  date: 2/5/2016, 1672 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1726.

[Abstract] In recent years, the utilization of stem cell therapy to regenerate cardiac tissue has been proposed as a possible strategy to treat cardiac damage (Gnecchi et al., 2012, Aguirre et al., 2013; Sanganalmath and Bolli, 2013). Although encouraging results have been obtained in experimental models, the ...

Aorta Ring Assay

Authors: Jing Jin
Jing JinAffiliation 1: Second Affiliated hospital Zhejiang University college of Medicine of clinic research center, Hangzhou, China
Affiliation 2: Cardiovascular key laboratory of Zhejiang province, Hangzhou, China
Bio-protocol author page: a3272
, Xinyang Hu
Xinyang HuAffiliation 1: Second Affiliated hospital Zhejiang University college of Medicine of clinic research center, Hangzhou, China
Affiliation 2: Second Affiliated hospital Zhejiang University college of Medicine of Department of science and education, Hangzhou, China
Bio-protocol author page: a3273
, Ling Zhang
Ling ZhangAffiliation: Cardiovascular key laboratory of Zhejiang province, Hangzhou, China
Bio-protocol author page: a3367
 and Jian'an Wang
Jian'an WangAffiliation 1: Second Affiliated hospital Zhejiang University college of Medicine of clinic research center, Hangzhou, China
Affiliation 2: Cardiovascular key laboratory of Zhejiang province, Hangzhou, China
For correspondence: jian_an_wang@yahoo.com
Bio-protocol author page: a3368
,  date: 7/5/2016, 1537 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1856.

[Abstract] Angiogenesis is the nature and the pathological process of blood vessels growth from pre-existing vascular bud. It plays an important role in cancer and cardiovascular disease. Aorta Ring Assay is an approach of Angiogenesis. In this experiment, we use the aorta of rat as material, clean surrounding ...

Permanent Occlusion of the Left Anterior Coronary Artery in the Rat

Authors: Mélanie Bousquenaud
Mélanie BousquenaudAffiliation: Laboratory of Pathology, Dept of Medicine, University of Fribourg, Fribourg, Switzerland
For correspondence: melanie.bousquenaud@unifr.ch
Bio-protocol author page: a2737
, Pierre-Yves Marie
Pierre-Yves MarieAffiliation: Nancyclotep, Experimental Imaging Platform, Nancy, France
Bio-protocol author page: a2738
 and Yvan Devaux
Yvan DevauxAffiliation: Laboratory of Cardiovascular Research, Luxembourg Institut of Health, Luxembourg, Luxembourg
Bio-protocol author page: a2642
,  date: 11/20/2015, 1366 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1663.

[Abstract] Left ventricular (LV) remodeling occurs in many patients after myocardial infarction (MI). LV remodeling is characterized by progressive ventricular dilatation and contractile dysfunction, consequently to cardiomyocyte hypertrophy and fibrosis. Despite reperfusion therapies, this pathophysiological ...
1 
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