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Single-step Marker Switching in
Schizosaccharomyces pombe
Using a Lithium Acetate Transformation Protocol
Authors:
Simon David Brown
Simon David Brown
Affiliation:
Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK
Bio-protocol author page:
a3916
and
Alexander Lorenz
Alexander Lorenz
Affiliation:
Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK
For correspondence:
a.lorenz@abdn.ac.uk
Bio-protocol author page:
a3917
,
date: 12/20/2016, 1321 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.2075
.
[Abstract] The ability to utilize different selectable markers for tagging or mutating multiple genes in
Schizosaccharomyces pombe
is hampered by the historical use of only two selectable markers,
ura4
+
and
kanMX6
; the latter conferring resistance to the antibiotic G418 (geneticin). More markers have been described ...
Chromatin Fractionation Assay in Fission Yeast
Authors:
Tatsuki Kunoh
Tatsuki Kunoh
Affiliation:
Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan
For correspondence:
tkunoh06@gmail.com
Bio-protocol author page:
a1508
and
Toshiyuki Habu
Toshiyuki Habu
Affiliation:
Radiation Biology Center, Kyoto University, Kyoto, Japan
Bio-protocol author page:
a1509
,
date: 7/20/2014, 3144 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1185
.
[Abstract] The protein recruitment onto chromatin is a critical process for DNA metabolism, including DNA replication, DNA repair and DNA recombination. Especially DNA modification enzymes and checkpoint proteins are loaded onto DNA damage sites in a context-dependent manner. In our recent study (Kunoh and Habu, ...
1
Chromatin Fractionation Assay in Fission Yeast
Authors:
Tatsuki Kunoh
Tatsuki Kunoh
Affiliation:
Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan
For correspondence:
tkunoh06@gmail.com
Bio-protocol author page:
a1508
and
Toshiyuki Habu
Toshiyuki Habu
Affiliation:
Radiation Biology Center, Kyoto University, Kyoto, Japan
Bio-protocol author page:
a1509
,
date: 7/20/2014, 3144 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1185
.
[Abstract] The protein recruitment onto chromatin is a critical process for DNA metabolism, including DNA replication, DNA repair and DNA recombination. Especially DNA modification enzymes and checkpoint proteins are loaded onto DNA damage sites in a context-dependent manner. In our recent study (Kunoh and Habu, ...
Single-step Marker Switching in
Schizosaccharomyces pombe
Using a Lithium Acetate Transformation Protocol
Authors:
Simon David Brown
Simon David Brown
Affiliation:
Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK
Bio-protocol author page:
a3916
and
Alexander Lorenz
Alexander Lorenz
Affiliation:
Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK
For correspondence:
a.lorenz@abdn.ac.uk
Bio-protocol author page:
a3917
,
date: 12/20/2016, 1321 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.2075
.
[Abstract] The ability to utilize different selectable markers for tagging or mutating multiple genes in
Schizosaccharomyces pombe
is hampered by the historical use of only two selectable markers,
ura4
+
and
kanMX6
; the latter conferring resistance to the antibiotic G418 (geneticin). More markers have been described ...
1
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