Mammalia

Protocol for Macrophage Depletion from Mice

Authors: Zuoxiang Xiao
Zuoxiang XiaoAffiliation: Cancer Inflammation Program, NCI-Frederick, NIH, Frederick, USA
For correspondence: xiaoz2@mail.nih.gov
Bio-protocol author page: a1391
and Qun Jiang
Qun JiangAffiliation: Cancer Inflammation Program, NCI-Frederick, NIH, Frederick, USA
Bio-protocol author page: a1392
, date: 6/5/2014, 5825 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1144.

[Abstract] Macrophage depletion has been used extensively to study autoimmune disease and more recently in tumor models. The clodronate-containing liposomes will be recognized as foreign particles and get engulfed by macrophages upon dosing into the animal by the chosen routes. Consequently, macrophages that have ...

	In vitro Culture of Human PBMCs
	Detection of Intracellular Reactive Oxygen Species (CM-H2DCFDA)
	Analysis of Protein Stability by the Cycloheximide Chase Assay
	Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation
	Dimethylmethylene Blue Assay (DMMB)
	Isolation of Human PBMCs
	BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry
	Analysis of Intestinal Permeability in Mice
	Isolation of Mouse Tumor-Infiltrating Leukocytes by Percoll Gradient Centrifugation
	Subcellular Fractionation of Cultured Human Cell Lines