Bacteria

A Filter Binding Assay to Quantify the Association of Cyclic di-GMP to Proteins

Authors: Disha Srivastava
Disha SrivastavaAffiliation: Department of Microbiology and Microbial Genetics, Michigan State University, East Lansing, USA
Bio-protocol author page: a1981
and Christopher M. Waters
Christopher M. WatersAffiliation: Department of Microbiology and Microbial Genetics, Michigan State University, East Lansing, USA
For correspondence: watersc3@msu.edu
Bio-protocol author page: a1982
, date: 2/5/2015, 3020 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1394.

[Abstract] Cyclic di-GMP (c-di-GMP) is a ubiquitous second messenger that regulates many processes in bacteria including biofilm formation, motility, and virulence (Hengge, 2009). Analysis of c-di-GMP binding properties of bacterial proteins is an important step to characterize c-di-GMP signaling pathways. C-di-GMP ...

Assay for Adherence of Vibrio cholerae to Eukaryotic Cell Lines

Authors: Amit K. Dey
Amit K. DeyAffiliation: Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, Council of Scientific and Industrial Research, Kolkata, India
Bio-protocol author page: a1296
, Abha Bhagat
Abha BhagatAffiliation: Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, Council of Scientific and Industrial Research, Kolkata, India
Bio-protocol author page: a1297
and Rukhsana Chowdhury
Rukhsana ChowdhuryAffiliation: Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, Council of Scientific and Industrial Research, Kolkata, India
For correspondence: rukhsana@iicb.res.in
Bio-protocol author page: a1298
, date: 4/20/2014, 3691 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1105.

[Abstract] Vibrio cholerae (V. cholerae) colonizes the intestinal epithelium and secretes cholera toxin (CT), a potent enterotoxin that causes severe fluid loss characteristic of the disease cholera. V. cholerae is a non-invasive Gram-negative bacterium that adheres to intestinal cells as well as a variety of ...

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