Bacteria

EMSA Analysis of DNA Binding By Rgg Proteins

Authors: Breah LaSarre
Breah LaSarreAffiliation: Microbiology and Immunology, University of Illinois at Chicago, Chicago, USA
Bio-protocol author page: a507
and Michael J. Federle
Michael J. FederleAffiliation: Medicinal Chemstry and Pharmacognosy, University of Illionois at Chicago, Chicago, USA
For correspondence: mfederle@uic.edu
Bio-protocol author page: a508
, date: 8/5/2013, 5067 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.838.

[Abstract] In bacteria, interaction of various proteins with DNA is essential for the regulation of specific target gene expression. Electrophoretic mobility shift assay (EMSA) is an in vitro approach allowing for the visualization of these protein-DNA interactions. Rgg proteins comprise a family of transcriptional ...

	Minimal Inhibitory Concentration (MIC) Assay for Acinetobacter baumannii
	Colony Immunoblotting Assay for Detection of Bacterial Cell-surface or Extracellular Proteins
	Measurement of Chlorophyll a and Carotenoids Concentration in Cyanobacteria
	Pyocyanin Extraction and Quantitative Analysis in Swarming Pseudomonas aeruginosa
	Gentamicin Protection Assay to Determine Bacterial Survival within Macrophages
	RNA Isolation from Synechocystis
	Isolation of Rhizosphere Bacterial Communities from Soil
	In vitro RNA-protein Binding Assay by UV Crosslinking
	Protein-peptide Interaction by Surface Plasmon Resonance
	Heterologous Expression and Purification of the Magnesium Transporter A (MgtA) in Escherichia coli