Welcome guest,
Sign in
Home
Mammalia
By Date
By View
miRNA Tagging and Affinity-purification (miRAP)
Author:
Miao He
Miao He
Affiliation:
Institutes of Brain Science, Fudan University, Shanghai, China
For correspondence:
hem@fudan.edu.cn
Bio-protocol author page:
a113
,
date: 10/5/2012, 5021 views,
1 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.265
.
[Abstract] MicroRNAs(miRNAs) are a group of endogenously expressed 20~23 nt small noncoding RNAs, which can directly regulate mRNA stability or translation in a sequence specific manner by incomplete base pairing at the 3’UTR of target mRNA, or indirectly affect transcriptional network by regulating transcription ...
1
miRNA Tagging and Affinity-purification (miRAP)
Author:
Miao He
Miao He
Affiliation:
Institutes of Brain Science, Fudan University, Shanghai, China
For correspondence:
hem@fudan.edu.cn
Bio-protocol author page:
a113
,
date: 10/5/2012, 5021 views,
1 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.265
.
[Abstract] MicroRNAs(miRNAs) are a group of endogenously expressed 20~23 nt small noncoding RNAs, which can directly regulate mRNA stability or translation in a sequence specific manner by incomplete base pairing at the 3’UTR of target mRNA, or indirectly affect transcriptional network by regulating transcription ...
1
Mammalia
Bovine
Cell line
Protein
Canine
Cell line
Protein
General
Cell line
Cell-based analysis
DNA
Morphology
Other compound
Physiology
Protein
RNA
Whole animal
Cell-based assay
Protein
Hamster
Cell line
DNA
Other compound
Protein
Whole animal
Physiology
Human
Olfactory tissue
Cell-based analysis
Bone
Other compound
Cardiovascular system
Cell-based analysis
Physiology
Cell line
Cell-based analysis
DNA
Isolation and culture
Lipid
Morphology
Other compound
Physiology
Protein
RNA
Chimeric mouse model
Cell-based analysis
Physiology
General
Protein
Hematopoietic system
Cell-based analysis
Protein
Immune system
Cell-based analysis
Muscle
Cell-based analysis
Tumor
Cell-based analysis
Physiology
RNA
Leporidae
Vasculature
Physiology
Monkey
Cell line
DNA
Physiology
Protein
Murine
Prostate
Cell-based analysis
Adipose
Cell-based analysis
Physiology
Bone
Cell-based analysis
Morphology
Physiology
Brain
Neurophysiology
Behavioral neuroscience
Cell-based analysis
DNA
Intracellular signalling
Lipid
Morphology
Neurobiology
Physiology
Protein
Breast
Biophysics
Cell-based analysis
Pathology
Physiology
Cell line
Carbohydrate
Cell-based analysis
DNA
Lipid
Morphology
Other compound
Physiology
Protein
RNA
Chimeric mouse model
Evolutionary Biology
Physiology
Ear
Physiology
Protein
Embryo
Cell-based analysis
DNA
Physiology
Eye
Cell-based analysis
Physiology
Heart
Protein
Hematopoietic system
Ex vivo analysis
Cell-based analysis
Intestine
Cell staining
Physiology
Protein
RNA
Kidney
Cell-based analysis
Morphology
Limb
Neurobiology
Liver
Carbohydrate
Cell-based analysis
Lipid
Physiology
Protein
Lung
Cell-based analysis
Morphology
Protein
Lymphatic system
Cell-based analysis
Muscle
Cell-based analysis
Nose
Physiology
Pancreas
Cell-based analysis
Physiology
Protein
Peritoneal cavity
Cell-based analysis
Pituitary
Physiology
Skin
Cell-based analysis
Physiology
Protein
Spinal cord
Cell-based analysis
Physiology
Spleen
Cell-based analysis
Physiology
Stomach
Cell-based analysis
Tail
DNA
Testes
Cell-based analysis
Tongue
Cell-based analysis
Physiology
Trachea
Physiology
Tumor
Carbohydrate
Cell-based analysis
Other compound
Physiology
Protein
Tissue analysis
Vagina
Pathology
Vasculature
Cell-based analysis
Physiology
Tissue analysis
Whole animal
Carbohydrate
Cell-based analysis
DNA
Infection
Lipid
Morphology
Neurobiology
Other compound
Physiology
Protein
RNA
Pig
Whole animal
Cell-based assay
Sheep
Embryo
DNA
Hot protocols of the week
In vitro
Culture of Human PBMCs
Detection of Intracellular Reactive Oxygen Species (CM-H2DCFDA)
Analysis of Protein Stability by the Cycloheximide Chase Assay
Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation
Dimethylmethylene Blue Assay (DMMB)
Isolation of Human PBMCs
BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry
Analysis of Intestinal Permeability in Mice
Isolation of Mouse Tumor-Infiltrating Leukocytes by Percoll Gradient Centrifugation
Subcellular Fractionation of Cultured Human Cell Lines