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Assessment of TCR-induced Sumoylation of PKC-θ
Authors:
Xudong Wang
Xudong Wang
Affiliation:
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Bio-protocol author page:
a3640
,
Zhilong Chen
Zhilong Chen
Affiliation:
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Bio-protocol author page:
a3641
,
Qilong Wang
Qilong Wang
Affiliation:
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Bio-protocol author page:
a3642
and
Yingqiu Li
Yingqiu Li
Affiliation:
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
For correspondence:
lsslyq@mail.sysu.edu.cn
Bio-protocol author page:
a3643
,
date: 10/20/2016, 865 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1979
.
[Abstract] Sumoylation controls many cellular processes. Protein kinase C-θ (PKC-θ), a member of the Ca
2+
-independent PKC subfamily of kinases, serves as a regulator of T cell activation by mediating the T cell antigen receptor (TCR)- and coreceptor CD28-induced activation of the transcription factors NF-κB and ...
Insulin Tolerance Test and Hyperinsulinemic-euglycemic Clamp
Authors:
Georgios K. Paschos
Georgios K. Paschos
Affiliation:
Pharmacology Department, University of Pennsylvania, Philadelphia, USA
For correspondence:
gpaschos@mail.med.upenn.edu
Bio-protocol author page:
a601
and
Garret A. FitzGerald
Garret A. FitzGerald
Affiliation:
Pharmacology Department, University of Pennsylvania, Philadelphia, USA
Bio-protocol author page:
a518
,
date: 5/20/2013, 3167 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.776
.
[Abstract] The two tests are used to evaluate
in vivo
sensitivity to insulin in mouse. The hypoerinsulinemic-euglycemic clamp provides information about the sensitivity to insulin in liver and other metabolically relevant tissues....
[Bio101] Activation of ER-regulated Fusion Proteins In Vivo
Author:
Jason Reuter
,
date: 12/20/2011, 6596 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.164
.
[Abstract] Regulatable protein fusions can be made by attaching the hormone binding domain of the estrogen receptor to the N or C-terminus of a protein of interest. Activation of such ER-fusion proteins
in vivo
can be achieved by daily administration of a synthetic ligand, 4-hydroxytamoxifen (4OHT), via intraperitoneal ...
1
[Bio101] Activation of ER-regulated Fusion Proteins In Vivo
Author:
Jason Reuter
,
date: 12/20/2011, 6596 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.164
.
[Abstract] Regulatable protein fusions can be made by attaching the hormone binding domain of the estrogen receptor to the N or C-terminus of a protein of interest. Activation of such ER-fusion proteins
in vivo
can be achieved by daily administration of a synthetic ligand, 4-hydroxytamoxifen (4OHT), via intraperitoneal ...
Insulin Tolerance Test and Hyperinsulinemic-euglycemic Clamp
Authors:
Georgios K. Paschos
Georgios K. Paschos
Affiliation:
Pharmacology Department, University of Pennsylvania, Philadelphia, USA
For correspondence:
gpaschos@mail.med.upenn.edu
Bio-protocol author page:
a601
and
Garret A. FitzGerald
Garret A. FitzGerald
Affiliation:
Pharmacology Department, University of Pennsylvania, Philadelphia, USA
Bio-protocol author page:
a518
,
date: 5/20/2013, 3167 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.776
.
[Abstract] The two tests are used to evaluate
in vivo
sensitivity to insulin in mouse. The hypoerinsulinemic-euglycemic clamp provides information about the sensitivity to insulin in liver and other metabolically relevant tissues....
Assessment of TCR-induced Sumoylation of PKC-θ
Authors:
Xudong Wang
Xudong Wang
Affiliation:
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Bio-protocol author page:
a3640
,
Zhilong Chen
Zhilong Chen
Affiliation:
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Bio-protocol author page:
a3641
,
Qilong Wang
Qilong Wang
Affiliation:
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Bio-protocol author page:
a3642
and
Yingqiu Li
Yingqiu Li
Affiliation:
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
For correspondence:
lsslyq@mail.sysu.edu.cn
Bio-protocol author page:
a3643
,
date: 10/20/2016, 865 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1979
.
[Abstract] Sumoylation controls many cellular processes. Protein kinase C-θ (PKC-θ), a member of the Ca
2+
-independent PKC subfamily of kinases, serves as a regulator of T cell activation by mediating the T cell antigen receptor (TCR)- and coreceptor CD28-induced activation of the transcription factors NF-κB and ...
1
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