Mammalia

In vivo Bioluminescence Imaging of Luciferase-labeled Cancer Cells

Authors: Marc Carceles-Cordon
Marc Carceles-CordonAffiliation: Department of Pathology, Icahn School of Medicine at Mount Sinai, New York, USA
Bio-protocol author page: a2976
, Irene Rodriguez-Fernandez
Irene Rodriguez-FernandezAffiliation: Department of Pathology, Icahn School of Medicine at Mount Sinai, New York, USA
Bio-protocol author page: a2977
, Veronica Rodriguez-Bravo
Veronica Rodriguez-BravoAffiliation: Department of Pathology, Icahn School of Medicine at Mount Sinai, New York, USA
Bio-protocol author page: a2978
, Carlos Cordon-Cardo
Carlos Cordon-CardoAffiliation: Department of Pathology, Icahn School of Medicine at Mount Sinai, New York, USA
Bio-protocol author page: a2979
and Josep Domingo-Domenech
Josep Domingo-DomenechAffiliation: Department of Pathology, Icahn School of Medicine at Mount Sinai, New York, USA
For correspondence: josep.domingo-domenech@mssm.edu
Bio-protocol author page: a2980
, date: 3/20/2016, 3832 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1762.

[Abstract] Over the past decade, in vivo bioluminescent imaging has emerged as a non-invasive and sensitive tool for studying ongoing biological processes within living organisms (Contag et al., 1997; Contag et al., 1998). Based on the detection and quantitation of the photons produced by the oxidation of luciferin ...

Preparation of Candida albicans Biofilms Using an in vivo Rat Central Venous Catheter Model

Authors: Heather T. Taff
Heather T. TaffAffiliation: Department of Medicine, University of Wisconsin, Madison, USA
For correspondence: htaff@wisc.edu
Bio-protocol author page: a333
, Karen Marchillo
Karen MarchilloAffiliation: Department of Medicine, University of Wisconsin, Madison, USA
Bio-protocol author page: a690
and David R. Andes
David R. AndesAffiliation: Department of Medicine, University of Wisconsin, Madison, USA
For correspondence: dra@medicine.wisc.edu
Bio-protocol author page: a689
, date: 7/20/2013, 2909 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.823.

[Abstract] In vivo biofilms grown on medical devices are necessary to understand the interactions of the fungal biofilm and the host environment in which it is most commonly found. This protocol describes a way to grow Candida albicans biofilms on the interior lumen of central venous catheters surgically implanted ...

Iron - Prussian Blue Reaction - Mallory’s Method

Author: Hani Jouihan
Hani JouihanAffiliation: Division of Endocrinology, Gerontology and Metabolism, Stanford University School of Medicine, Stanford, USA
For correspondence: hanijouihan@hotmail.com
Bio-protocol author page: a52
, date: 7/5/2012, 11696 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.222.

[Abstract] Purpose: To demonstrate ferric iron in tissue sections. Small amounts of iron are found normally in spleen and bone marrow. Excessive amounts are present in hemochromatosis, with deposits found in the liver and pancreas, hemosiderosis, with deposits in the liver, spleen, and lymph nodes.
Principle: The ...

	In vitro Culture of Human PBMCs
	Detection of Intracellular Reactive Oxygen Species (CM-H2DCFDA)
	Analysis of Protein Stability by the Cycloheximide Chase Assay
	Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation
	Dimethylmethylene Blue Assay (DMMB)
	Isolation of Human PBMCs
	BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry
	Analysis of Intestinal Permeability in Mice
	Isolation of Mouse Tumor-Infiltrating Leukocytes by Percoll Gradient Centrifugation
	Subcellular Fractionation of Cultured Human Cell Lines