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Identification of Methylated Deoxyadenosines in Genomic DNA by dA6m DNA Immunoprecipitation

Authors: Magdalena J. Koziol
Magdalena J. KoziolAffiliation 1: Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK
Affiliation 2: Department of Zoology, University of Cambridge, Cambridge, UK
For correspondence: mjk39@cam.ac.uk
Bio-protocol author page: a3666
, Charles R. Bradshaw
Charles R. BradshawAffiliation: Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK
Bio-protocol author page: a3667
, George E. Allen
George E. AllenAffiliation: Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK
Bio-protocol author page: a3668
, Ana S. H. Costa
Ana S. H. CostaAffiliation: Medical Research Council Cancer Unit, University of Cambridge, Hutchison/MRC Research Centre, Cambridge, UK
Bio-protocol author page: a3669
 and Christian Frezza
Christian FrezzaAffiliation: Medical Research Council Cancer Unit, University of Cambridge, Hutchison/MRC Research Centre, Cambridge, UK
Bio-protocol author page: a3670
,  date: 11/5/2016, 1143 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1990.

[Abstract] dA6m DNA immunoprecipitation followed by deep sequencing (DIP-Seq) is a key tool in identifying and studying the genome-wide distribution of N6-methyldeoxyadenosine (dA6m). The precise function of this novel DNA modification remains to be fully elucidated, but it is known to be absent from transcriptional ...

Generation of Mitochondrial-nuclear eXchange Mice via Pronuclear Transfer

Authors: Robert A. Kesterson
Robert A. KestersonAffiliation: Department of Genetics, University of Alabama, Birmingham, USA
For correspondence: kesterso@uab.edu
Bio-protocol author page: a3629
, Larry W. Johnson
Larry W. JohnsonAffiliation: Department of Genetics, University of Alabama, Birmingham, USA
Bio-protocol author page: a3630
, Laura J. Lambert
Laura J. LambertAffiliation: Department of Genetics, University of Alabama, Birmingham, USA
Bio-protocol author page: a3631
, Jay L. Vivian
Jay L. VivianAffiliation: Department of Pathology, University of Kansas Medical Center, Kansas City, USA
Bio-protocol author page: a3632
, Danny R. Welch
Danny R. WelchAffiliation: Department of Cancer Biology, University of Kansas Cancer Center, Kansas City, USA
For correspondence: dwelch@kumc.edu
Bio-protocol author page: a3633
 and Scott W. Ballinger
Scott W. BallingerAffiliation: Division of Molecular and Cellular Pathology, University of Alabama, Birmingham, USA
For correspondence: sballing@uab.edu
Bio-protocol author page: a3634
,  date: 10/20/2016, 1460 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1976.

[Abstract] The mitochondrial paradigm for common disease proposes that mitochondrial DNA (mtDNA) sequence variation can contribute to disease susceptibility and progression. To test this concept, we developed the Mitochondrial-nuclear eXchange (MNX) model, in which isolated embryonic pronuclei from one strain ...

A Quick, No Frills Approach to Mouse Genotyping

Author: Manuel E. Lopez
Manuel E. LopezAffiliation: Department of Developmental Biology, Stanford University, Stanford, USA
For correspondence: manduardo@gmail.com
Bio-protocol author page: a74
,  date: 8/5/2012, 12325 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.244.

[Abstract] Mice are extremely powerful mammalian genetic model organisms for basic and medical research, but managing a colony of transgenic mice is time consuming and expensive, many times requiring the help of dedicated technicians. Slow and laborious genotyping procedures add to the hassle. Outsourcing is costly ...

[Bio101] Chromatin Immunoprecipitation (ChIP) Protocol from Tissue

Author: Nabila Aboulaich,  date: 1/20/2011, 10773 views, 2 Q&A
DOI: https://doi.org/10.21769/BioProtoc.21.

[Abstract] ...
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A Quick, No Frills Approach to Mouse Genotyping

Author: Manuel E. Lopez
Manuel E. LopezAffiliation: Department of Developmental Biology, Stanford University, Stanford, USA
For correspondence: manduardo@gmail.com
Bio-protocol author page: a74
,  date: 8/5/2012, 12325 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.244.

[Abstract] Mice are extremely powerful mammalian genetic model organisms for basic and medical research, but managing a colony of transgenic mice is time consuming and expensive, many times requiring the help of dedicated technicians. Slow and laborious genotyping procedures add to the hassle. Outsourcing is costly ...

[Bio101] Chromatin Immunoprecipitation (ChIP) Protocol from Tissue

Author: Nabila Aboulaich,  date: 1/20/2011, 10773 views, 2 Q&A
DOI: https://doi.org/10.21769/BioProtoc.21.

[Abstract] ...

Generation of Mitochondrial-nuclear eXchange Mice via Pronuclear Transfer

Authors: Robert A. Kesterson
Robert A. KestersonAffiliation: Department of Genetics, University of Alabama, Birmingham, USA
For correspondence: kesterso@uab.edu
Bio-protocol author page: a3629
, Larry W. Johnson
Larry W. JohnsonAffiliation: Department of Genetics, University of Alabama, Birmingham, USA
Bio-protocol author page: a3630
, Laura J. Lambert
Laura J. LambertAffiliation: Department of Genetics, University of Alabama, Birmingham, USA
Bio-protocol author page: a3631
, Jay L. Vivian
Jay L. VivianAffiliation: Department of Pathology, University of Kansas Medical Center, Kansas City, USA
Bio-protocol author page: a3632
, Danny R. Welch
Danny R. WelchAffiliation: Department of Cancer Biology, University of Kansas Cancer Center, Kansas City, USA
For correspondence: dwelch@kumc.edu
Bio-protocol author page: a3633
 and Scott W. Ballinger
Scott W. BallingerAffiliation: Division of Molecular and Cellular Pathology, University of Alabama, Birmingham, USA
For correspondence: sballing@uab.edu
Bio-protocol author page: a3634
,  date: 10/20/2016, 1460 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1976.

[Abstract] The mitochondrial paradigm for common disease proposes that mitochondrial DNA (mtDNA) sequence variation can contribute to disease susceptibility and progression. To test this concept, we developed the Mitochondrial-nuclear eXchange (MNX) model, in which isolated embryonic pronuclei from one strain ...
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Mammalia
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