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Tumorigenicity Assay in Nude Mice

Authors: Feng Du
Feng DuAffiliation: State key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China
Bio-protocol author page: a4714
, Xiaodi Zhao
Xiaodi ZhaoAffiliation: State key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China
For correspondence: leedyzhao@fmmu.edu.cn
Bio-protocol author page: a4715
 and Daiming Fan
Daiming FanAffiliation: State key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi’an, China
Bio-protocol author page: a4716
,  date: 7/5/2017, 120 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2364.

[Abstract] Tumorigenicity refers to the ability of cultured cells to develop viable tumors in immune-deficient animals. The goal of this protocol is to illustrate tumorigenicity assay by subcutaneous tumor-cell-transplantation in nude mice. Target cells are transplanted to 6-week-old nude mice subcutaneously and ...

Determining Leukocyte Origins Using Parabiosis in the PyMT Breast Tumor Model

Authors: Ruth A. Franklin
Ruth A. FranklinAffiliation 1: Immunology Program, Memorial Sloan Kettering Cancer Center, New York, USA
Affiliation 2: Graduate Program in Immunology and Microbial Pathogenesis, Weill Cornell Graduate School of Medical Sciences, Cornell University, New York, USA
Present address: , Yale University School of Medicine, New Haven, USA
For correspondence: ruth.franklin@yale.edu
Bio-protocol author page: a2488
 and Ming O. Li
Ming O. LiAffiliation: Immunology Program, Memorial Sloan Kettering Cancer Center, New York, USA
For correspondence: lim@mskcc.org
Bio-protocol author page: a1463
,  date: 8/20/2015, 2434 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1567.

[Abstract] Tumors develop in a complex microenvironment alongside numerous cell types that impact their survival. Immune cells make up a large proportion of these accessory cells and many are known to promote tumor progression. Macrophages, in particular, are associated with poor patient prognosis and are therefore ...

Isolation of FAP Cells from Mouse Dystrophic Skeletal Muscle Using Fluorescence Activated Cell Sorting

Authors: Nicoletta Cordani
Nicoletta CordaniAffiliation 1: Scientific Institute, Lecco, Italy
Affiliation 2: Department of Health of Science, University of Milano-Bicocca, Monza, Italy
Bio-protocol author page: a1797
, Viviana Pisa
Viviana PisaAffiliation: Department of Biomedical and Clinical Sciences, University of Milano, Milan, Italy
Bio-protocol author page: a1798
, Laura Pozzi
Laura PozziAffiliation: Scientific Institute, Lecco, Italy
Bio-protocol author page: a1799
 and Clara Sciorati
Clara ScioratiAffiliation: Division of Regenerative Medicine, Ospedale San Raffaele, Milan, Italy
For correspondence: sciorati.clara@hsr.it
Bio-protocol author page: a1800
,  date: 11/20/2014, 3160 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1292.

[Abstract] A population of muscle resident CD45-, CD31- cells expressing the mesenchymal PDGF receptor alpha (PDGFRα) as well as Sca-1 was first isolated in healthy mouse muscles in Uezumi et al. (2010). In the same year, Joe et al. (2010) identified and purified fibro-adipogenic precursors (FAPs), cells located ...

Isolation and in vivo Transfer of Antigen Presenting Cells

Authors: Pooja Arora
Pooja AroraAffiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, USA
Bio-protocol author page: a1709
, Shalu Sharma Kharkwal
Shalu Sharma KharkwalAffiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, USA
Bio-protocol author page: a1710
 and Steven A. Porcelli
Steven A. PorcelliAffiliation: Department of Medicine, Albert Einstein College of Medicine, NY, USA
For correspondence: steven.porcelli@einstein.yu.edu
Bio-protocol author page: a1711
,  date: 10/5/2014, 3795 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1260.

[Abstract] Transfer of antigen presenting cells in vivo is a method used by immunologists to examine the potency of antigen presentation by a selected population of cells. This method is most commonly used to analyze presentation of protein antigens to MHC class I or II restricted T cells, but it can also be used ...

Vaccine-induced Cytokine Production Detected by Luminex Multiplex Analysis

Authors: Rachel Perret
Rachel PerretAffiliation: Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland
For correspondence: rperret@fredhutch.org
Bio-protocol author page: a1603
, Sophie R. Sierro
Sophie R. SierroAffiliation: Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland
Bio-protocol author page: a1604
, Natalia K. Botelho
Natalia K. BotelhoAffiliation: Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland
Bio-protocol author page: a1605
, Stephanie Corgnac
Stephanie CorgnacAffiliation: Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland
Bio-protocol author page: a1606
, Alena Donda
Alena DondaAffiliation: Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland
Bio-protocol author page: a1607
 and Pedro Romero
Pedro RomeroAffiliation: Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland
Bio-protocol author page: a1608
,  date: 8/20/2014, 2752 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1217.

[Abstract] Different vaccine and adjuvant combinations are known to rapidly induce antigen presenting cell (APC) maturation and pro-inflammatory cytokine and production, which in turn play an important role in the priming of antigen-specific T cells. Measuring cytokine production systemically in the serum fails ...

[Bio101] Isolation of Endothelial Cells from Mice

Author: Huan Pang
Huan PangAffiliation: Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, USA
For correspondence: pang_huan@hotmail.com
Bio-protocol author page: a48
,  date: 4/20/2012, 11592 views, 4 Q&A
DOI: https://doi.org/10.21769/BioProtoc.205.

[Abstract] Endothelial cells line the entire circulatory system, from the heart to the smallest capillary. In this protocol, attaining and maintaining vitality of the organ is critical. Minimizing the processing time between removing the organ and beginning the digestions will improve yields as cells are more ...
1 

[Bio101] Isolation of Endothelial Cells from Mice

Author: Huan Pang
Huan PangAffiliation: Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, USA
For correspondence: pang_huan@hotmail.com
Bio-protocol author page: a48
,  date: 4/20/2012, 11592 views, 4 Q&A
DOI: https://doi.org/10.21769/BioProtoc.205.

[Abstract] Endothelial cells line the entire circulatory system, from the heart to the smallest capillary. In this protocol, attaining and maintaining vitality of the organ is critical. Minimizing the processing time between removing the organ and beginning the digestions will improve yields as cells are more ...

Isolation and in vivo Transfer of Antigen Presenting Cells

Authors: Pooja Arora
Pooja AroraAffiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, USA
Bio-protocol author page: a1709
, Shalu Sharma Kharkwal
Shalu Sharma KharkwalAffiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, USA
Bio-protocol author page: a1710
 and Steven A. Porcelli
Steven A. PorcelliAffiliation: Department of Medicine, Albert Einstein College of Medicine, NY, USA
For correspondence: steven.porcelli@einstein.yu.edu
Bio-protocol author page: a1711
,  date: 10/5/2014, 3795 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1260.

[Abstract] Transfer of antigen presenting cells in vivo is a method used by immunologists to examine the potency of antigen presentation by a selected population of cells. This method is most commonly used to analyze presentation of protein antigens to MHC class I or II restricted T cells, but it can also be used ...

Isolation of FAP Cells from Mouse Dystrophic Skeletal Muscle Using Fluorescence Activated Cell Sorting

Authors: Nicoletta Cordani
Nicoletta CordaniAffiliation 1: Scientific Institute, Lecco, Italy
Affiliation 2: Department of Health of Science, University of Milano-Bicocca, Monza, Italy
Bio-protocol author page: a1797
, Viviana Pisa
Viviana PisaAffiliation: Department of Biomedical and Clinical Sciences, University of Milano, Milan, Italy
Bio-protocol author page: a1798
, Laura Pozzi
Laura PozziAffiliation: Scientific Institute, Lecco, Italy
Bio-protocol author page: a1799
 and Clara Sciorati
Clara ScioratiAffiliation: Division of Regenerative Medicine, Ospedale San Raffaele, Milan, Italy
For correspondence: sciorati.clara@hsr.it
Bio-protocol author page: a1800
,  date: 11/20/2014, 3160 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1292.

[Abstract] A population of muscle resident CD45-, CD31- cells expressing the mesenchymal PDGF receptor alpha (PDGFRα) as well as Sca-1 was first isolated in healthy mouse muscles in Uezumi et al. (2010). In the same year, Joe et al. (2010) identified and purified fibro-adipogenic precursors (FAPs), cells located ...

Vaccine-induced Cytokine Production Detected by Luminex Multiplex Analysis

Authors: Rachel Perret
Rachel PerretAffiliation: Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland
For correspondence: rperret@fredhutch.org
Bio-protocol author page: a1603
, Sophie R. Sierro
Sophie R. SierroAffiliation: Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland
Bio-protocol author page: a1604
, Natalia K. Botelho
Natalia K. BotelhoAffiliation: Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland
Bio-protocol author page: a1605
, Stephanie Corgnac
Stephanie CorgnacAffiliation: Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland
Bio-protocol author page: a1606
, Alena Donda
Alena DondaAffiliation: Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland
Bio-protocol author page: a1607
 and Pedro Romero
Pedro RomeroAffiliation: Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland
Bio-protocol author page: a1608
,  date: 8/20/2014, 2752 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1217.

[Abstract] Different vaccine and adjuvant combinations are known to rapidly induce antigen presenting cell (APC) maturation and pro-inflammatory cytokine and production, which in turn play an important role in the priming of antigen-specific T cells. Measuring cytokine production systemically in the serum fails ...

Determining Leukocyte Origins Using Parabiosis in the PyMT Breast Tumor Model

Authors: Ruth A. Franklin
Ruth A. FranklinAffiliation 1: Immunology Program, Memorial Sloan Kettering Cancer Center, New York, USA
Affiliation 2: Graduate Program in Immunology and Microbial Pathogenesis, Weill Cornell Graduate School of Medical Sciences, Cornell University, New York, USA
Present address: , Yale University School of Medicine, New Haven, USA
For correspondence: ruth.franklin@yale.edu
Bio-protocol author page: a2488
 and Ming O. Li
Ming O. LiAffiliation: Immunology Program, Memorial Sloan Kettering Cancer Center, New York, USA
For correspondence: lim@mskcc.org
Bio-protocol author page: a1463
,  date: 8/20/2015, 2434 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1567.

[Abstract] Tumors develop in a complex microenvironment alongside numerous cell types that impact their survival. Immune cells make up a large proportion of these accessory cells and many are known to promote tumor progression. Macrophages, in particular, are associated with poor patient prognosis and are therefore ...

Tumorigenicity Assay in Nude Mice

Authors: Feng Du
Feng DuAffiliation: State key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China
Bio-protocol author page: a4714
, Xiaodi Zhao
Xiaodi ZhaoAffiliation: State key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China
For correspondence: leedyzhao@fmmu.edu.cn
Bio-protocol author page: a4715
 and Daiming Fan
Daiming FanAffiliation: State key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi’an, China
Bio-protocol author page: a4716
,  date: 7/5/2017, 120 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2364.

[Abstract] Tumorigenicity refers to the ability of cultured cells to develop viable tumors in immune-deficient animals. The goal of this protocol is to illustrate tumorigenicity assay by subcutaneous tumor-cell-transplantation in nude mice. Target cells are transplanted to 6-week-old nude mice subcutaneously and ...
1 
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