Fluorescence Recovery After Photobleaching (FRAP) in the Fission Yeast Nucleus

Authors: Petrina Delivani
Petrina DelivaniAffiliation: Department of Molecular Cell Biology and Genetics, Max Planck Institute, Dresden, Germany
For correspondence: delivani@mpi-cbg.de
Bio-protocol author page: a914
, Mariola R. Chacón
Mariola R. ChacónAffiliation: Department of Molecular Cell Biology and Genetics, Max Planck Institute, Dresden, Germany
For correspondence: mchacon@mpi-cbg.de
Bio-protocol author page: a915
, Britta Schroth-Diez
Britta Schroth-DiezAffiliation: Department of Molecular Cell Biology and Genetics, Max Planck Institute, Dresden, Germany
For correspondence: schroth@mpi-cbg.de
Bio-protocol author page: a916
and Iva M. Tolić-Nørrelykke
Iva M. Tolić-NørrelykkeAffiliation: Department of Molecular Cell Biology and Genetics, Max Planck Institute, Dresden, Germany
For correspondence: tolic@mpi-cbg.de
Bio-protocol author page: a493
, date: 10/20/2013, 3864 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.941.

[Abstract] We use fluorescence recovery after photobleaching (FRAP) to calculate the diffusion coefficient of GFP in the nucleoplasm of fission yeast. The FRAP method can be generally used to measure the mobility of proteins inside the cell or its organelles.
In our experiment we only measured the diffusion of ...

Fluorescence Recovery After Photobleaching (FRAP) in the Fission Yeast Nucleus

Authors: Petrina Delivani
Petrina DelivaniAffiliation: Department of Molecular Cell Biology and Genetics, Max Planck Institute, Dresden, Germany
For correspondence: delivani@mpi-cbg.de
Bio-protocol author page: a914
, Mariola R. Chacón
Mariola R. ChacónAffiliation: Department of Molecular Cell Biology and Genetics, Max Planck Institute, Dresden, Germany
For correspondence: mchacon@mpi-cbg.de
Bio-protocol author page: a915
, Britta Schroth-Diez
Britta Schroth-DiezAffiliation: Department of Molecular Cell Biology and Genetics, Max Planck Institute, Dresden, Germany
For correspondence: schroth@mpi-cbg.de
Bio-protocol author page: a916
and Iva M. Tolić-Nørrelykke
Iva M. Tolić-NørrelykkeAffiliation: Department of Molecular Cell Biology and Genetics, Max Planck Institute, Dresden, Germany
For correspondence: tolic@mpi-cbg.de
Bio-protocol author page: a493
, date: 10/20/2013, 3864 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.941.

Fungi

Schizosaccharomyces pombe

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