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Northern Blot of tRNA in Yeast
Author:
Yuehua Wei
Yuehua Wei
Affiliation:
No.3 People's Hospital, Shanghai Jiao Tong University, Shanghai, China
For correspondence:
weiyh.sjtu.edu@gmail.com
Bio-protocol author page:
a49
,
date: 4/5/2013, 5763 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.464
.
[Abstract] tRNAs are small RNAs around 70-90 nt. tRNAs are different from many other small RNAs in that they are very abundant, which makes it difficult to study their transcriptional regulation by traditional northern blot. Traditional Northern blot involves incorporation of radioactive nucleotides through polymerization, ...
Metabolic Labeling of Yeast RNA with Radioactive Uracil
Author:
Yuehua Wei
Yuehua Wei
Affiliation:
No.3 People's Hospital, Shanghai Jiao Tong University, Shanghai, China
For correspondence:
weiyh.sjtu.edu@gmail.com
Bio-protocol author page:
a49
,
date: 4/5/2013, 4188 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.623
.
[Abstract] To examine gene expression, Northern blot or Real-Time PCR can be used to detect low abundant RNA such as mRNA. However, for high abundant RNAs such as rRNA and tRNA, Northern blot will not be able to discriminate the newly synthesized RNA from total RNA. Therefore, metabolic labeling is necessary to ...
A Simple Preparation of RNA from Yeast by Hot Phenol for Northern Blot
Author:
Yuehua Wei
Yuehua Wei
Affiliation:
Department of Pharmacology, Cancer Institute of New Jersey, UMDNJ Robert Wood Johnson Medical School, Piscataway, USA
For correspondence:
weiyh.sjtu.edu@gmail.com
Bio-protocol author page:
a49
,
date: 6/20/2012, 7470 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.209
.
[Abstract] Compared to several expensive RNA extraction kits, the following protocol provides an economic and simple method for researchers to extract yeast RNA. This method can achieve RNA quality that is sufficient for most northern blot studies in yeast....
[Bio101] RNA Extraction from Yeast
Author:
Wei Zheng
Wei Zheng
Affiliation:
Keck Biotech Services, Yale University, New Haven, USA
For correspondence:
wei.zheng.madison@gmail.com
Bio-protocol author page:
a10
,
date: 10/5/2011, 5601 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.138
.
[Abstract] ...
[Bio101] Yeast in situ Hybridization
Author:
Wei Zheng
Wei Zheng
Affiliation:
Keck Biotech Services, Yale University, New Haven, USA
For correspondence:
wei.zheng.madison@gmail.com
Bio-protocol author page:
a10
,
date: 9/20/2011, 5474 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.134
.
[Abstract] This protocol describes the procedure to label a certain mRNA species in yeast cells with fluorescence probes and prepare slides for visualization under microscope. Probe design/synthesis and microscopy operation are not covered....
[Bio101] RNA Preparation for Microarray Experiments
Author:
Zongtian Tong
Zongtian Tong
Affiliation:
Department of Cell Biology, Center for Metabolism and Obesity Research, Johns Hopkins School of Medicine, Baltimore, USA
For correspondence:
tongzong@gmail.com
Bio-protocol author page:
a14
,
date: 1/20/2011, 8565 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.19
.
[Abstract] This protocol describes a simple and relatively general method to extract total RNA from a yeast culture using the Qiagen RNeasy kit. In it, total RNA is treated with DNase I and cleaned up to be suitable for microarray experiments. Therefore, this protocol can be used to generate a large yield of high ...
1
[Bio101] RNA Preparation for Microarray Experiments
Author:
Zongtian Tong
Zongtian Tong
Affiliation:
Department of Cell Biology, Center for Metabolism and Obesity Research, Johns Hopkins School of Medicine, Baltimore, USA
For correspondence:
tongzong@gmail.com
Bio-protocol author page:
a14
,
date: 1/20/2011, 8565 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.19
.
[Abstract] This protocol describes a simple and relatively general method to extract total RNA from a yeast culture using the Qiagen RNeasy kit. In it, total RNA is treated with DNase I and cleaned up to be suitable for microarray experiments. Therefore, this protocol can be used to generate a large yield of high ...
A Simple Preparation of RNA from Yeast by Hot Phenol for Northern Blot
Author:
Yuehua Wei
Yuehua Wei
Affiliation:
Department of Pharmacology, Cancer Institute of New Jersey, UMDNJ Robert Wood Johnson Medical School, Piscataway, USA
For correspondence:
weiyh.sjtu.edu@gmail.com
Bio-protocol author page:
a49
,
date: 6/20/2012, 7470 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.209
.
[Abstract] Compared to several expensive RNA extraction kits, the following protocol provides an economic and simple method for researchers to extract yeast RNA. This method can achieve RNA quality that is sufficient for most northern blot studies in yeast....
Northern Blot of tRNA in Yeast
Author:
Yuehua Wei
Yuehua Wei
Affiliation:
No.3 People's Hospital, Shanghai Jiao Tong University, Shanghai, China
For correspondence:
weiyh.sjtu.edu@gmail.com
Bio-protocol author page:
a49
,
date: 4/5/2013, 5763 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.464
.
[Abstract] tRNAs are small RNAs around 70-90 nt. tRNAs are different from many other small RNAs in that they are very abundant, which makes it difficult to study their transcriptional regulation by traditional northern blot. Traditional Northern blot involves incorporation of radioactive nucleotides through polymerization, ...
[Bio101] RNA Extraction from Yeast
Author:
Wei Zheng
Wei Zheng
Affiliation:
Keck Biotech Services, Yale University, New Haven, USA
For correspondence:
wei.zheng.madison@gmail.com
Bio-protocol author page:
a10
,
date: 10/5/2011, 5601 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.138
.
[Abstract] ...
[Bio101] Yeast in situ Hybridization
Author:
Wei Zheng
Wei Zheng
Affiliation:
Keck Biotech Services, Yale University, New Haven, USA
For correspondence:
wei.zheng.madison@gmail.com
Bio-protocol author page:
a10
,
date: 9/20/2011, 5474 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.134
.
[Abstract] This protocol describes the procedure to label a certain mRNA species in yeast cells with fluorescence probes and prepare slides for visualization under microscope. Probe design/synthesis and microscopy operation are not covered....
Metabolic Labeling of Yeast RNA with Radioactive Uracil
Author:
Yuehua Wei
Yuehua Wei
Affiliation:
No.3 People's Hospital, Shanghai Jiao Tong University, Shanghai, China
For correspondence:
weiyh.sjtu.edu@gmail.com
Bio-protocol author page:
a49
,
date: 4/5/2013, 4188 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.623
.
[Abstract] To examine gene expression, Northern blot or Real-Time PCR can be used to detect low abundant RNA such as mRNA. However, for high abundant RNAs such as rRNA and tRNA, Northern blot will not be able to discriminate the newly synthesized RNA from total RNA. Therefore, metabolic labeling is necessary to ...
1
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