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Measuring UV-induced Mutagenesis at the
CAN1
Locus in
Saccharomyces cerevisiae
Authors:
Ildiko Unk
Ildiko Unk
Affiliation:
The Institute of Genetics, Biological Research Center of The Hungarian Academy of Sciences, Szeged, Hungary
For correspondence:
unk.ildiko@brc.mta.hu
Bio-protocol author page:
a1741
and
Andreea Daraba
Andreea Daraba
Affiliation:
The Institute of Genetics, Biological Research Center of The Hungarian Academy of Sciences, Szeged, Hungary
Bio-protocol author page:
a1742
,
date: 10/20/2014, 2967 views,
1 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1272
.
[Abstract] There are several methods to measure the capacity of yeast cell to respond to environmental impacts on their genome by mutating it. One frequently used method involves the detection of forward mutations in the
CAN1
gene. The
CAN1
gene encodes for an arginine permease that is responsible for the uptake ...
Subcellular Fractionation Using Accudenz Gradient to Separate ER/Golgi in Yeast
Author:
Zongtian Tong
Zongtian Tong
Affiliation:
Department of Cell Biology, Center for Metabolism and Obesity Research, Johns Hopkins School of Medicine, Baltimore, USA
For correspondence:
tongzong@gmail.com
Bio-protocol author page:
a14
,
date: 1/20/2012, 8503 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.20
.
[Abstract] This protocol describes how to separate the endoplasmic reticulum (ER) and Golgi apparatus in yeast cells using a subcellular fractionation approach with an Accudenz gradient....
[Bio101] Yeast Vacuole Staining with FM4-64
Author:
Zongtian Tong
Zongtian Tong
Affiliation:
Department of Cell Biology, Center for Metabolism and Obesity Research, Johns Hopkins School of Medicine, Baltimore, USA
For correspondence:
tongzong@gmail.com
Bio-protocol author page:
a14
,
date: 1/5/2011, 15341 views,
1 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.18
.
[Abstract] The lipophilic probe, FM 4-64 does not fluoresce much in water but fluoresces strongly after binding to the outer plasma membrane, providing clear and distinguishable plasma membrane staining. The binding is rapid and reversible. In this protocol vacuoles in yeast cells are stained with the FM4-64 dye, ...
1
[Bio101] Yeast Vacuole Staining with FM4-64
Author:
Zongtian Tong
Zongtian Tong
Affiliation:
Department of Cell Biology, Center for Metabolism and Obesity Research, Johns Hopkins School of Medicine, Baltimore, USA
For correspondence:
tongzong@gmail.com
Bio-protocol author page:
a14
,
date: 1/5/2011, 15341 views,
1 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.18
.
[Abstract] The lipophilic probe, FM 4-64 does not fluoresce much in water but fluoresces strongly after binding to the outer plasma membrane, providing clear and distinguishable plasma membrane staining. The binding is rapid and reversible. In this protocol vacuoles in yeast cells are stained with the FM4-64 dye, ...
Subcellular Fractionation Using Accudenz Gradient to Separate ER/Golgi in Yeast
Author:
Zongtian Tong
Zongtian Tong
Affiliation:
Department of Cell Biology, Center for Metabolism and Obesity Research, Johns Hopkins School of Medicine, Baltimore, USA
For correspondence:
tongzong@gmail.com
Bio-protocol author page:
a14
,
date: 1/20/2012, 8503 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.20
.
[Abstract] This protocol describes how to separate the endoplasmic reticulum (ER) and Golgi apparatus in yeast cells using a subcellular fractionation approach with an Accudenz gradient....
Measuring UV-induced Mutagenesis at the
CAN1
Locus in
Saccharomyces cerevisiae
Authors:
Ildiko Unk
Ildiko Unk
Affiliation:
The Institute of Genetics, Biological Research Center of The Hungarian Academy of Sciences, Szeged, Hungary
For correspondence:
unk.ildiko@brc.mta.hu
Bio-protocol author page:
a1741
and
Andreea Daraba
Andreea Daraba
Affiliation:
The Institute of Genetics, Biological Research Center of The Hungarian Academy of Sciences, Szeged, Hungary
Bio-protocol author page:
a1742
,
date: 10/20/2014, 2967 views,
1 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1272
.
[Abstract] There are several methods to measure the capacity of yeast cell to respond to environmental impacts on their genome by mutating it. One frequently used method involves the detection of forward mutations in the
CAN1
gene. The
CAN1
gene encodes for an arginine permease that is responsible for the uptake ...
1
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