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Metabolite and Fatty Acid Analysis of Yeast Cells and Culture Supernatants
Authors:
Liwei Chen
Liwei Chen
Affiliation:
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, Singapore
Bio-protocol author page:
a1609
and
Wei Ning Chen
Wei Ning Chen
Affiliation:
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, Singapore
For correspondence:
wnchen@ntu.edu.sg
Bio-protocol author page:
a1610
,
date: 9/5/2014, 5102 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1219
.
[Abstract] Metabolite and fatty acid analysis play important roles in evaluating the metabolic state of microorganisms. To examine the growth state and metabolism response of cells to environmental stress or genetic modification, intracellular and extracellular metabolites including fatty acids are usually analyzed ...
Metabolic Labeling of Yeast Sphingolipids with Radioactive D-erythro-[4,5-
3
H]dihydrosphingosine
Authors:
Takefumi Karashima
Takefumi Karashima
Affiliation:
Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan
Bio-protocol author page:
a762
,
Kentaro Kajiwara
Kentaro Kajiwara
Affiliation:
Department of Oncogene Research, Research Institute for Microbial Diseases, Osaka University, Suita, Japan
Bio-protocol author page:
a763
and
Kouichi Funato
Kouichi Funato
Affiliation:
Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan
For correspondence:
kfunato@hiroshima-u.ac.jp
Bio-protocol author page:
a499
,
date: 8/20/2013, 3617 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.862
.
[Abstract] Yeast sphingolipids can be metabolically labeled with exogenously added radioactive precursors. Here we describe a method to label ceramides and complex sphingolipids of
Saccharomyces cerevisiae
with a radioactive ceramide precursor, D-erythro-[4, 5-
3
H]dihydrosphingosine. This protocol is used to study ...
1
Metabolite and Fatty Acid Analysis of Yeast Cells and Culture Supernatants
Authors:
Liwei Chen
Liwei Chen
Affiliation:
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, Singapore
Bio-protocol author page:
a1609
and
Wei Ning Chen
Wei Ning Chen
Affiliation:
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, Singapore
For correspondence:
wnchen@ntu.edu.sg
Bio-protocol author page:
a1610
,
date: 9/5/2014, 5102 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1219
.
[Abstract] Metabolite and fatty acid analysis play important roles in evaluating the metabolic state of microorganisms. To examine the growth state and metabolism response of cells to environmental stress or genetic modification, intracellular and extracellular metabolites including fatty acids are usually analyzed ...
Metabolic Labeling of Yeast Sphingolipids with Radioactive D-erythro-[4,5-
3
H]dihydrosphingosine
Authors:
Takefumi Karashima
Takefumi Karashima
Affiliation:
Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan
Bio-protocol author page:
a762
,
Kentaro Kajiwara
Kentaro Kajiwara
Affiliation:
Department of Oncogene Research, Research Institute for Microbial Diseases, Osaka University, Suita, Japan
Bio-protocol author page:
a763
and
Kouichi Funato
Kouichi Funato
Affiliation:
Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan
For correspondence:
kfunato@hiroshima-u.ac.jp
Bio-protocol author page:
a499
,
date: 8/20/2013, 3617 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.862
.
[Abstract] Yeast sphingolipids can be metabolically labeled with exogenously added radioactive precursors. Here we describe a method to label ceramides and complex sphingolipids of
Saccharomyces cerevisiae
with a radioactive ceramide precursor, D-erythro-[4, 5-
3
H]dihydrosphingosine. This protocol is used to study ...
1
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