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Assaying Glycogen and Trehalose in Yeast
Authors:
Yuping Chen
Yuping Chen
Affiliation:
Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY, USA
Bio-protocol author page:
a4763
and
Bruce Futcher
Bruce Futcher
Affiliation:
Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY, USA
For correspondence:
bfutcher@gmail.com
Bio-protocol author page:
a4764
,
date: 7/5/2017, 115 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.2371
.
[Abstract] Organisms store carbohydrates in several forms. In yeast, carbohydrates are stored in glycogen (a multi-branched polysaccharide) and in trehalose (a disaccharide). As in other organisms, the amount of stored carbohydrate varies dramatically with physiological state, and accordingly, an assay of stored ...
Extraction and Quantification of Polyphosphate in the Budding Yeast
Saccharomyces cerevisiae
Authors:
David Canadell
David Canadell
Affiliation:
Departament de Bioquímica i Biologia Molecular and Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Barcelona, Spain
Bio-protocol author page:
a3328
,
Samuel Bru
Samuel Bru
Affiliation:
Departament de Ciències Bàsiques and Facultat de Medicina i Ciències de la Salut, Universitat Internacional de Catalunya, Sant Cugat del Vallès, Barcelona, Spain
Bio-protocol author page:
a3329
,
Josep Clotet
Josep Clotet
Affiliation:
Departament de Ciències Bàsiques and Facultat de Medicina i Ciències de la Salut, Universitat Internacional de Catalunya, Sant Cugat del Vallès, Barcelona, Spain
For correspondence:
jclotet@uic.es
Bio-protocol author page:
a3330
and
Joaquín Ariño
Joaquín Ariño
Affiliation:
Departament de Bioquímica i Biologia Molecular and Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Barcelona, Spain
For correspondence:
joaquin.arino@uab.es
Bio-protocol author page:
a3331
,
date: 7/20/2016, 1591 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1874
.
[Abstract] Inorganic polyphosphate (polyP) is a linear polymer present in both prokaryotic and eukaryotic organisms and made from three to hundreds of orthophosphate residues linked by phosphoanhydride bonds. The biological role of this molecule goes beyond serving as Pi store or energy source to replace ATP. ...
[Bio101] How to Use an Avestin Emulsiflex C3 Homogenizer to Disrupt Cells
Author:
Zongtian Tong
Zongtian Tong
Affiliation:
Department of Cell Biology, Center for Metabolism and Obesity Research, Johns Hopkins School of Medicine, Baltimore, USA
For correspondence:
tongzong@gmail.com
Bio-protocol author page:
a14
,
date: 1/5/2011, 12233 views,
1 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.11
.
[Abstract] The EmulsiFlex-C3 homogenizer is powered by an electric motor. The pump does not require a compressor for it to run. This equipment can be used to disrupt cells at a large scale. The EmulsiFlex-C3 has a fixed flow-through capacity of 3 L/h. It has the ability to process samples as small as 10 ml. The ...
1
[Bio101] How to Use an Avestin Emulsiflex C3 Homogenizer to Disrupt Cells
Author:
Zongtian Tong
Zongtian Tong
Affiliation:
Department of Cell Biology, Center for Metabolism and Obesity Research, Johns Hopkins School of Medicine, Baltimore, USA
For correspondence:
tongzong@gmail.com
Bio-protocol author page:
a14
,
date: 1/5/2011, 12233 views,
1 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.11
.
[Abstract] The EmulsiFlex-C3 homogenizer is powered by an electric motor. The pump does not require a compressor for it to run. This equipment can be used to disrupt cells at a large scale. The EmulsiFlex-C3 has a fixed flow-through capacity of 3 L/h. It has the ability to process samples as small as 10 ml. The ...
Extraction and Quantification of Polyphosphate in the Budding Yeast
Saccharomyces cerevisiae
Authors:
David Canadell
David Canadell
Affiliation:
Departament de Bioquímica i Biologia Molecular and Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Barcelona, Spain
Bio-protocol author page:
a3328
,
Samuel Bru
Samuel Bru
Affiliation:
Departament de Ciències Bàsiques and Facultat de Medicina i Ciències de la Salut, Universitat Internacional de Catalunya, Sant Cugat del Vallès, Barcelona, Spain
Bio-protocol author page:
a3329
,
Josep Clotet
Josep Clotet
Affiliation:
Departament de Ciències Bàsiques and Facultat de Medicina i Ciències de la Salut, Universitat Internacional de Catalunya, Sant Cugat del Vallès, Barcelona, Spain
For correspondence:
jclotet@uic.es
Bio-protocol author page:
a3330
and
Joaquín Ariño
Joaquín Ariño
Affiliation:
Departament de Bioquímica i Biologia Molecular and Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Barcelona, Spain
For correspondence:
joaquin.arino@uab.es
Bio-protocol author page:
a3331
,
date: 7/20/2016, 1591 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1874
.
[Abstract] Inorganic polyphosphate (polyP) is a linear polymer present in both prokaryotic and eukaryotic organisms and made from three to hundreds of orthophosphate residues linked by phosphoanhydride bonds. The biological role of this molecule goes beyond serving as Pi store or energy source to replace ATP. ...
Assaying Glycogen and Trehalose in Yeast
Authors:
Yuping Chen
Yuping Chen
Affiliation:
Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY, USA
Bio-protocol author page:
a4763
and
Bruce Futcher
Bruce Futcher
Affiliation:
Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY, USA
For correspondence:
bfutcher@gmail.com
Bio-protocol author page:
a4764
,
date: 7/5/2017, 115 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.2371
.
[Abstract] Organisms store carbohydrates in several forms. In yeast, carbohydrates are stored in glycogen (a multi-branched polysaccharide) and in trehalose (a disaccharide). As in other organisms, the amount of stored carbohydrate varies dramatically with physiological state, and accordingly, an assay of stored ...
1
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