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In vitro
Analysis for Macrophage Binding and Pro-inflammatory Responses to
Candida albicans
Author:
Mohlopheni J. Marakalala
Mohlopheni J. Marakalala
Affiliation:
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Harvard University, Boston, USA
For correspondence:
mjmaraka@hsph.harvard.edu
Bio-protocol author page:
a1356
,
date: 5/5/2014, 2758 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1123
.
[Abstract] Macrophage recognition of
Candida albicans
(
C. albicans
) is facilitated by pattern recognition receptors that interact with the fungal pathogen associated molecular patterns (PAMPs). Dectin-1 is the major macrophage receptor that is known to recognize fungal Beta-glucans leading to induction of various ...
Preparation of
Candida albicans
Biofilms for Transmission Electron Microscopy
Authors:
Heather T. Taff
Heather T. Taff
Affiliation:
Department of Medicine, University of Wisconsin, Madison, USA
For correspondence:
htaff@wisc.edu
Bio-protocol author page:
a333
and
David R. Andes
David R. Andes
Affiliation:
Department of Medicine, University of Wisconsin, Madison, USA
For correspondence:
dra@medicine.wisc.edu
Bio-protocol author page:
a689
,
date: 7/20/2013, 4153 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.822
.
[Abstract] Transmission Electron Microscopy is a form of microscopy that allows for imaging of distinct portions of an individual cell. For
Candida albicans
biofilms, it is often used to visualize the cell walls of fixed samples of yeast and hyphae. This protocol describes how to grow, harvest, and fix
Candida ...
Preparation of Mitochondria from
Candida albicans
Authors:
Victoria L Hewitt
Victoria L Hewitt
Affiliation:
Department of Biochemistry and Molecular Biology and Department of Microbiology, Monash University, Melbourne (Clayton), Australia
For correspondence:
victoria.hewitt@monash.edu
Bio-protocol author page:
a235
,
Trevor Lithgow
Trevor Lithgow
Affiliation:
Department of Biochemistry and Molecular Biology and Department of Microbiology, Monash University, Melbourne (Clayton), Australia
Bio-protocol author page:
a236
and
Ana Traven
Ana Traven
Affiliation:
Department of Biochemistry and Molecular Biology, Monash University, Melbourne (Clayton), Australia
For correspondence:
ana.traven@monash.edu
Bio-protocol author page:
a237
,
date: 2/20/2013, 4010 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.330
.
[Abstract] Based on the methods of (Daum
et al.
, 1982) and (Hewitt
et al.
, 2012), we have established the use of
Candida albicans
as a new model system to study mitochondrial biogenesis. This dimorphic yeast provides an excellent system to investigate the coordination of mitochondrial biogenesis with other cellular ...
1
Preparation of
Candida albicans
Biofilms for Transmission Electron Microscopy
Authors:
Heather T. Taff
Heather T. Taff
Affiliation:
Department of Medicine, University of Wisconsin, Madison, USA
For correspondence:
htaff@wisc.edu
Bio-protocol author page:
a333
and
David R. Andes
David R. Andes
Affiliation:
Department of Medicine, University of Wisconsin, Madison, USA
For correspondence:
dra@medicine.wisc.edu
Bio-protocol author page:
a689
,
date: 7/20/2013, 4153 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.822
.
[Abstract] Transmission Electron Microscopy is a form of microscopy that allows for imaging of distinct portions of an individual cell. For
Candida albicans
biofilms, it is often used to visualize the cell walls of fixed samples of yeast and hyphae. This protocol describes how to grow, harvest, and fix
Candida ...
Preparation of Mitochondria from
Candida albicans
Authors:
Victoria L Hewitt
Victoria L Hewitt
Affiliation:
Department of Biochemistry and Molecular Biology and Department of Microbiology, Monash University, Melbourne (Clayton), Australia
For correspondence:
victoria.hewitt@monash.edu
Bio-protocol author page:
a235
,
Trevor Lithgow
Trevor Lithgow
Affiliation:
Department of Biochemistry and Molecular Biology and Department of Microbiology, Monash University, Melbourne (Clayton), Australia
Bio-protocol author page:
a236
and
Ana Traven
Ana Traven
Affiliation:
Department of Biochemistry and Molecular Biology, Monash University, Melbourne (Clayton), Australia
For correspondence:
ana.traven@monash.edu
Bio-protocol author page:
a237
,
date: 2/20/2013, 4010 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.330
.
[Abstract] Based on the methods of (Daum
et al.
, 1982) and (Hewitt
et al.
, 2012), we have established the use of
Candida albicans
as a new model system to study mitochondrial biogenesis. This dimorphic yeast provides an excellent system to investigate the coordination of mitochondrial biogenesis with other cellular ...
In vitro
Analysis for Macrophage Binding and Pro-inflammatory Responses to
Candida albicans
Author:
Mohlopheni J. Marakalala
Mohlopheni J. Marakalala
Affiliation:
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Harvard University, Boston, USA
For correspondence:
mjmaraka@hsph.harvard.edu
Bio-protocol author page:
a1356
,
date: 5/5/2014, 2758 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1123
.
[Abstract] Macrophage recognition of
Candida albicans
(
C. albicans
) is facilitated by pattern recognition receptors that interact with the fungal pathogen associated molecular patterns (PAMPs). Dectin-1 is the major macrophage receptor that is known to recognize fungal Beta-glucans leading to induction of various ...
1
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