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Fluorescently Labelled Aerolysin (FLAER) Labelling of
Candida albicans
Cells
Authors:
Sneh Lata Singh
Sneh Lata Singh
Affiliation:
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India
Bio-protocol author page:
a4573
and
Sneha Sudha Komath
Sneha Sudha Komath
Affiliation:
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India
For correspondence:
sskomath@mail.jnu.ac.in
Bio-protocol author page:
a4574
,
date: 6/5/2017, 312 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.2303
.
[Abstract] In this protocol we describe a nonradiolabelled labelling of GPI anchor in
Candida albicans
. The method uses a fluorescent probe to bind specifically to GPI anchors so that the level of GPI-anchored proteins at the cell surface can be measured. The labelling does not need permeabilization of cells and ...
Detection of Hog1 Phosphorylation in
Candida albicans
in Response to an Antifungal Protein
Authors:
Brigitte ME Hayes
Brigitte ME Hayes
Affiliation:
La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Australia
Bio-protocol author page:
a1668
and
Nicole L van der Weerden
Nicole L van der Weerden
Affiliation:
La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Australia
For correspondence:
n.vanderweerden@latrobe.edu.au
Bio-protocol author page:
a1669
,
date: 9/20/2014, 2623 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1244
.
[Abstract] It is becoming increasingly apparent that stress signalling is important for tolerance of fungal species to antifungal chemicals and proteins. The high-osmolarity glycerol (HOG) pathway responds to a number of stressors including osmotic and oxidative stress. This protocol describes a method to detect ...
Immunoblot Analysis of Histone H4 Acetylation and Histone H2A Phosphorylation in
Candida albicans
Authors:
Michael Tscherner
Michael Tscherner
Affiliation:
Max F. Perutz Laboratories, Campus Vienna Biocenter, Medical University of Vienna, Vienna, Austria
Bio-protocol author page:
a929
and
Karl Kuchler
Karl Kuchler
Affiliation:
Max F. Perutz Laboratories, Campus Vienna Biocenter, Medical University of Vienna, Vienna, Austria
For correspondence:
karl.kuchler@meduniwien.ac.at
Bio-protocol author page:
a496
,
date: 10/20/2013, 3617 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.943
.
[Abstract] Posttranslational modifications of histones are required for different processes including transcription, replication and DNA damage repair. This protocol describes the preparation of a whole-cell extracts for the fungal pathogen
Candida albicans
. Furthermore, the extract is used to detect lysine acetylation ...
Candida albicans
Mitochondrial Protein Import Assay
Authors:
Victoria L Hewitt
Victoria L Hewitt
Affiliation:
Department of Biochemistry and Molecular Biology and Department of Microbiology, Monash University, Melbourne (Clayton), Australia
For correspondence:
victoria.hewitt@monash.edu
Bio-protocol author page:
a235
,
Trevor Lithgow
Trevor Lithgow
Affiliation:
Department of Biochemistry and Molecular Biology and Department of Microbiology, Monash University, Melbourne (Clayton), Australia
Bio-protocol author page:
a236
and
Ana Traven
Ana Traven
Affiliation:
Department of Biochemistry and Molecular Biology, Monash University, Melbourne (Clayton), Australia
For correspondence:
ana.traven@monash.edu
Bio-protocol author page:
a237
,
date: 2/20/2013, 3911 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.331
.
[Abstract] We have established the use of
Candida albicans
as a new model system to study mitochondrial biogenesis. This dimorphic yeast provides an excellent system to investigate the coordination of mitochondrial biogenesis with other cellular networks including cellular metabolism and the cell cycle. Unlike ...
1
Candida albicans
Mitochondrial Protein Import Assay
Authors:
Victoria L Hewitt
Victoria L Hewitt
Affiliation:
Department of Biochemistry and Molecular Biology and Department of Microbiology, Monash University, Melbourne (Clayton), Australia
For correspondence:
victoria.hewitt@monash.edu
Bio-protocol author page:
a235
,
Trevor Lithgow
Trevor Lithgow
Affiliation:
Department of Biochemistry and Molecular Biology and Department of Microbiology, Monash University, Melbourne (Clayton), Australia
Bio-protocol author page:
a236
and
Ana Traven
Ana Traven
Affiliation:
Department of Biochemistry and Molecular Biology, Monash University, Melbourne (Clayton), Australia
For correspondence:
ana.traven@monash.edu
Bio-protocol author page:
a237
,
date: 2/20/2013, 3911 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.331
.
[Abstract] We have established the use of
Candida albicans
as a new model system to study mitochondrial biogenesis. This dimorphic yeast provides an excellent system to investigate the coordination of mitochondrial biogenesis with other cellular networks including cellular metabolism and the cell cycle. Unlike ...
Immunoblot Analysis of Histone H4 Acetylation and Histone H2A Phosphorylation in
Candida albicans
Authors:
Michael Tscherner
Michael Tscherner
Affiliation:
Max F. Perutz Laboratories, Campus Vienna Biocenter, Medical University of Vienna, Vienna, Austria
Bio-protocol author page:
a929
and
Karl Kuchler
Karl Kuchler
Affiliation:
Max F. Perutz Laboratories, Campus Vienna Biocenter, Medical University of Vienna, Vienna, Austria
For correspondence:
karl.kuchler@meduniwien.ac.at
Bio-protocol author page:
a496
,
date: 10/20/2013, 3617 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.943
.
[Abstract] Posttranslational modifications of histones are required for different processes including transcription, replication and DNA damage repair. This protocol describes the preparation of a whole-cell extracts for the fungal pathogen
Candida albicans
. Furthermore, the extract is used to detect lysine acetylation ...
Detection of Hog1 Phosphorylation in
Candida albicans
in Response to an Antifungal Protein
Authors:
Brigitte ME Hayes
Brigitte ME Hayes
Affiliation:
La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Australia
Bio-protocol author page:
a1668
and
Nicole L van der Weerden
Nicole L van der Weerden
Affiliation:
La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Australia
For correspondence:
n.vanderweerden@latrobe.edu.au
Bio-protocol author page:
a1669
,
date: 9/20/2014, 2623 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1244
.
[Abstract] It is becoming increasingly apparent that stress signalling is important for tolerance of fungal species to antifungal chemicals and proteins. The high-osmolarity glycerol (HOG) pathway responds to a number of stressors including osmotic and oxidative stress. This protocol describes a method to detect ...
Fluorescently Labelled Aerolysin (FLAER) Labelling of
Candida albicans
Cells
Authors:
Sneh Lata Singh
Sneh Lata Singh
Affiliation:
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India
Bio-protocol author page:
a4573
and
Sneha Sudha Komath
Sneha Sudha Komath
Affiliation:
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India
For correspondence:
sskomath@mail.jnu.ac.in
Bio-protocol author page:
a4574
,
date: 6/5/2017, 312 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.2303
.
[Abstract] In this protocol we describe a nonradiolabelled labelling of GPI anchor in
Candida albicans
. The method uses a fluorescent probe to bind specifically to GPI anchors so that the level of GPI-anchored proteins at the cell surface can be measured. The labelling does not need permeabilization of cells and ...
1
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