Bacteria

Preparation of Everted Membrane Vesicles from Escherichia coli Cells

Author: Marina Verkhovskaya
Marina VerkhovskayaAffiliation: Institute of Biotechnology, PO Box 65 (Viikinkaari 1) FIN-00014 University of Helsinki, Helsinki, Finland
For correspondence: Marina.Verkhovskaya@Helsinki.Fi
Bio-protocol author page: a4446
, date: 5/5/2017, 349 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2254.

[Abstract] The protocol for obtaining electrically sealed membrane vesicles from E. coli cells is presented. Proton pumps such as Complex I, quinol oxidase, and ATPase are active in the obtained vesicles. Quality of the preparation was tested by monitoring the electric potential generated by these pumps. ...

Quantification of Bacterial Fatty Acids by Extraction and Methylation

Authors: Mark Politz
Mark PolitzAffiliation: Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, USA
Bio-protocol author page: a938
, Rebecca Lennen
Rebecca LennenAffiliation: Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, USA
Bio-protocol author page: a939
and Brian Pfleger
Brian PflegerAffiliation: Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, USA
For correspondence: pfleger@engr.wisc.edu
Bio-protocol author page: a557
, date: 11/5/2013, 5537 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.950.

[Abstract] This protocol describes two similar methods for the extraction and methylation of fatty acids from bacterial cultures. The acid derivatization protocol (Lennen et al., 2013; Bligh and Dyer, 1959) results in the extraction and methylation of all fatty acids, both free and bound, from a bacterial culture, ...

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