Bacteria

Construction and Screening of a Transposon Insertion Library of Yersinia enterocolitica (YeO3-R1)

Authors: Maria Pajunen
Maria PajunenAffiliation: Department of Biosciences, Division of Biochemistry and Biotechnology, University of Helsinki, Helsinki, Finland
Bio-protocol author page: a79
, Elise Pinta
Elise PintaAffiliation: Department of Medical Biochemistry and Genetics, University of Turku, Turku, Finland
Bio-protocol author page: a80
and Mikael Skurnik
Mikael SkurnikAffiliation: Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland
For correspondence: mikael.skurnik@helsinki.fi
Bio-protocol author page: a78
, date: 8/5/2012, 6596 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.246.

[Abstract] The Mu-transposon system is one of the best characterized transposition systems. Under minimal in vitro set-up, Mu transposition requires only a simple reaction buffer, MuA transposase protein, mini-Mu transposon DNA (donor) and target DNA. The reaction proceeds via initial assembly of the transposition ...

Construction and Screening of a Transposon Insertion Library of Yersinia enterocolitica (YeO3-R1)

Authors: Maria Pajunen
Maria PajunenAffiliation: Department of Biosciences, Division of Biochemistry and Biotechnology, University of Helsinki, Helsinki, Finland
Bio-protocol author page: a79
, Elise Pinta
Elise PintaAffiliation: Department of Medical Biochemistry and Genetics, University of Turku, Turku, Finland
Bio-protocol author page: a80
and Mikael Skurnik
Mikael SkurnikAffiliation: Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland
For correspondence: mikael.skurnik@helsinki.fi
Bio-protocol author page: a78
, date: 8/5/2012, 6596 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.246.

Bacteria

Yersinia enterocolitica

Yersinia pestis

Zobellia

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