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Thermostability Measurement of an α-Glucosidase Using a Classical Activity-based Assay and a Novel Thermofluor Method
Authors:
Karin Ernits
Karin Ernits
Affiliation:
Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia
Bio-protocol author page:
a4705
,
Katrin Viigand
Katrin Viigand
Affiliation:
Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia
Bio-protocol author page:
a4706
,
Triinu Visnapuu
Triinu Visnapuu
Affiliation:
Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia
Bio-protocol author page:
a4707
,
Kristina Põšnograjeva
Kristina Põšnograjeva
Affiliation:
Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia
Bio-protocol author page:
a4708
and
Tiina Alamäe
Tiina Alamäe
Affiliation:
Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia
For correspondence:
talamae@ebc.ee
Bio-protocol author page:
a4709
,
date: 6/20/2017, 228 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.2349
.
[Abstract] α-glucosidases (including maltases and isomaltases) are enzymes which release glucose from a set of α-glucosidic substrates. Their catalytic activity, substrate specificity and thermostability can be assayed using this trait. Thermostability of proteins can also be determined using a high-throughput ...
1
Thermostability Measurement of an α-Glucosidase Using a Classical Activity-based Assay and a Novel Thermofluor Method
Authors:
Karin Ernits
Karin Ernits
Affiliation:
Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia
Bio-protocol author page:
a4705
,
Katrin Viigand
Katrin Viigand
Affiliation:
Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia
Bio-protocol author page:
a4706
,
Triinu Visnapuu
Triinu Visnapuu
Affiliation:
Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia
Bio-protocol author page:
a4707
,
Kristina Põšnograjeva
Kristina Põšnograjeva
Affiliation:
Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia
Bio-protocol author page:
a4708
and
Tiina Alamäe
Tiina Alamäe
Affiliation:
Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia
For correspondence:
talamae@ebc.ee
Bio-protocol author page:
a4709
,
date: 6/20/2017, 228 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.2349
.
[Abstract] α-glucosidases (including maltases and isomaltases) are enzymes which release glucose from a set of α-glucosidic substrates. Their catalytic activity, substrate specificity and thermostability can be assayed using this trait. Thermostability of proteins can also be determined using a high-throughput ...
1
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