Bacteria

Protein Expression Protocol for an Adenylate Cyclase Anchored by a Vibrio Quorum Sensing Receptor

Authors: Stephanie Beltz
Stephanie BeltzAffiliation: Pharmazeutisches Institut der Universität Tübingen, Pharmazeutische Biochemie, Tübingen, Germany
For correspondence: stephanie.beltz@uni-tuebingen.de
Bio-protocol author page: a4015
and Joachim E. Schultz
Joachim E. SchultzAffiliation: Pharmazeutisches Institut der Universität Tübingen, Pharmazeutische Biochemie, Tübingen, Germany
For correspondence: joachim.schultz@uni-tuebingen.de
Bio-protocol author page: a4016
, date: 1/20/2017, 941 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2112.

[Abstract] The direct regulation of a mycobacterial adenylate cyclase (Rv1625c) via exchange of its membrane anchor by the quorum sensing receptor CqsS (Vibrio harveyi) has recently been reported (Beltz et al., 2016). This protocol describes the expression and membrane preparation for these chimeric proteins....

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