Bacteria

Transformation of Thermus Species by Natural Competence

Authors: Alba Blesa
Alba BlesaAffiliation: Department of Molecular Biology, Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid - Consejo Superior de Investigaciones Científicas, Madrid, Spain
Bio-protocol author page: a3710
and José Berenguer
José BerenguerAffiliation: Department of Molecular Biology, Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid - Consejo Superior de Investigaciones Científicas, Madrid, Spain
For correspondence: jberenguer@cbm.csic.es
Bio-protocol author page: a3711
, date: 11/20/2016, 1020 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2007.

[Abstract] Many Thermus species harbour genomes scourged with horizontally transferred signatures. Thermus thermophilus (Tth) has been characterized as naturally competent. The transformation protocol described here is based on the maximum DNA uptake rates registered at mid-exponential phase (OD₆₀₀ 0.3-0.4). Here ...

Cell-to-cell DNA Transfer among Thermus Species

Authors: Alba Blesa
Alba BlesaAffiliation: Department of Molecular Biology, Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid - Consejo Superior de Investigaciones Científicas, Madrid, Spain
Bio-protocol author page: a3710
and José Berenguer
José BerenguerAffiliation: Department of Molecular Biology, Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid - Consejo Superior de Investigaciones Científicas, Madrid, Spain
For correspondence: jberenguer@cbm.csic.es
Bio-protocol author page: a3711
, date: 11/20/2016, 1150 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2006.

[Abstract] The ability to transfer DNA via direct cell-to-cell contact-dependent process similar to conjugation has been described in Thermus thermophilus (Tth). Here, we detail the mating experiment protocol involving the lateral transfer of thermostable antibiotic resistance markers (i.e., kanamycin: Km^R; hygromycin: ...

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