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Mammalia

Active Cdk5 Immunoprecipitation and Kinase Assay

Authors: Andrew N. Bankston
Andrew N. BankstonAffiliation 1: Department of Pharmacology, Emory University, Atlanta, GA
Affiliation 2: Department of Neurological Surgery, University of Louisville, Louisville, KY
Bio-protocol author page: a4746
, Li Ku
Li KuAffiliation: Department of Pharmacology, Emory University, Atlanta, GA
Bio-protocol author page: a4747
 and Yue Feng
Yue FengAffiliation: Department of Pharmacology, Emory University, Atlanta, GA
For correspondence: yfeng@emory.edu
Bio-protocol author page: a4748
,  date: 7/5/2017, 113 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2363.

[Abstract] Cdk5 activity is regulated by the amounts of two activator proteins, p35 and p39 (Tsai et al., 1994; Zheng et al., 1998; Humbert et al., 2000). The p35-Cdk5 and p39-Cdk5 complexes have differing sensitivity to salt and detergent concentrations (Hisanaga and Saito, 2003; Sato et al., 2007; Yamada et ...

Microvesicle Isolation from Rat Brain Extract Treated Human Mesenchymal Stem Cells

Authors: Ji Yong Lee
Ji Yong LeeAffiliation: Institute for BioMedical Convergence, Catholic Kwandong University-International St. Mary’s Hospital, Incheon-si, Republic of Korea
Bio-protocol author page: a4768
, Seong-Mi Choi
Seong-Mi ChoiAffiliation: Institute for BioMedical Convergence, Catholic Kwandong University-International St. Mary’s Hospital, Incheon-si, Republic of Korea
Bio-protocol author page: a4769
 and Han-Soo Kim
Han-Soo KimAffiliation: Department of Biomedical Sciences, College of Medical Convergence, Catholic Kwandong University, Gangneung-si, Gangwon-do, Republic of Korea
For correspondence: hankim63@gmail.com
Bio-protocol author page: a4770
,  date: 7/5/2017, 146 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2375.

[Abstract] Microvesicle (MVs) are submicron-sized membranous vesicles that are either actively released from cells via secretory compartments or shed from cell surface membranes. MVs are generated by many cell types and serve as vehicles that transfer biological information (e.g., protein, mRNA, and miRNA) to ...

Formaldehyde Fixation of Extracellular Matrix Protein Layers for Enhanced Primary Cell Growth

Authors: Natalia V. Andreeva
Natalia V. AndreevaAffiliation: Laboratory of Stem and Progenitor Cell Biology, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia
Bio-protocol author page: a4766
 and Alexander V. Belyavsky
Alexander V. BelyavskyAffiliation: Laboratory of Stem and Progenitor Cell Biology, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia
For correspondence: abelyavs@yahoo.com
Bio-protocol author page: a4767
,  date: 7/5/2017, 139 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2374.

[Abstract] Coating tissue culture vessels with the components of the extracellular matrix such as fibronectin and collagens provides a more natural environment for primary cells in vitro and stimulates their proliferation. However, the effects of such protein layers are usually rather modest, which might be explained ...

Soft Agar Colony Formation Assay as a Hallmark of Carcinogenesis

Authors: Feng Du
Feng DuAffiliation: State key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi’an, China
Bio-protocol author page: a4714
, Xiaodi Zhao
Xiaodi ZhaoAffiliation: State key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi’an, China
For correspondence: leedyzhao@fmmu.edu.cn
Bio-protocol author page: a4715
 and Daiming Fan
Daiming FanAffiliation: State key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi’an, China
Bio-protocol author page: a4716
,  date: 6/20/2017, 284 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2351.

[Abstract] Soft agar colony formation assay is established to estimate the anchorage-independent growth ability of cells. In this assay, a bottom layer of agar with complete media is poured and solidified first, followed by an upper layer containing a specified number of cells suspended in medium-agar mixture. ...

Loading of Extracellular Vesicles with Chemically Stabilized Hydrophobic siRNAs for the Treatment of Disease in the Central Nervous System

Authors: Reka A. Haraszti
Reka A. HarasztiAffiliation 1: RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA, USA
Affiliation 2: Department of Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
Bio-protocol author page: a4671
, Andrew Coles
Andrew ColesAffiliation 1: RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA, USA
Affiliation 2: Department of Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
Bio-protocol author page: a4672
, Neil Aronin
Neil AroninAffiliation 1: RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA, USA
Affiliation 2: Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA
Bio-protocol author page: a4673
, Anastasia Khvorova
Anastasia KhvorovaAffiliation 1: RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA, USA
Affiliation 2: Department of Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
Bio-protocol author page: a4674
 and Marie-Cécile Didiot
Marie-Cécile DidiotAffiliation 1: RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA, USA
Affiliation 2: Department of Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
For correspondence: marie.didiot@umassmed.edu
Bio-protocol author page: a4675
,  date: 6/20/2017, 221 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2338.

[Abstract] Efficient delivery of oligonucleotide therapeutics, i.e., siRNAs, to the central nervous system represents a significant barrier to their clinical advancement for the treatment of neurological disorders. Small, endogenous extracellular vesicles were shown to be able to transport lipids, proteins and ...

DNA Fiber Assay upon Treatment with Ultraviolet Radiations

Authors: Alfano Luigi
Alfano LuigiAffiliation: Oncology Research Center of Mercogliano (CROM), Istituto Nazionale Per Lo Studio E La Cura Dei Tumori “Fondazione Giovanni Pascale”, IRCCS, Naples, Italy
Bio-protocol author page: a4568
, Antonio Giordano
Antonio GiordanoAffiliation: Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and Technology, Department of Biology, Temple University, Philadelphia PA, USA
For correspondence: giordano@temple.edu
Bio-protocol author page: a4569
 and Francesca Pentimalli
Francesca PentimalliAffiliation: Oncology Research Center of Mercogliano (CROM), Istituto Nazionale Per Lo Studio E La Cura Dei Tumori “Fondazione Giovanni Pascale”, IRCCS, Naples, Italy
Bio-protocol author page: a4570
,  date: 6/5/2017, 317 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2301.

[Abstract] Genome stability is continuously challenged by a wide range of DNA damaging factors. To promote a correct DNA repair and cell survival, cells orchestrate a coordinated and finely tuned cascade of events collectively known as the DNA damage Response (DDR). Ultra Violet (UV) rays are among the main environmental ...

Formation of Minimised Hairpin Template-transcribing Dumbbell Vectors for Small RNA Expression

Authors: Xiaoou Jiang
Xiaoou JiangAffiliation: Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Block MD4, Level 5, 5 Science Drive 2, Singapore 117597, Singapore
Bio-protocol author page: a4599
 and Volker Patzel
Volker PatzelAffiliation: Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Block MD4, Level 5, 5 Science Drive 2, Singapore 117597, Singapore
For correspondence: micvp@nus.edu.sg
Bio-protocol author page: a4600
,  date: 6/5/2017, 287 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2313.

[Abstract] A major barrier for using non-viral vectors for gene therapy is the short duration of transgene expression in postmitotic tissues. Previous studies showed transgene expression from conventional plasmid fell to sub-therapeutic level shortly after delivery even though the vector DNA was retained, suggesting ...

Fluorometric Estimation of Glutathione in Cultured Microglial Cell Lysate

Authors: Vikas Singh
Vikas SinghAffiliation 1: Immunotoxicology Laboratory, Food Drug and Chemical Toxicology Group and Nanotherapeutics & Nanomaterial Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), 31-Vishvigyan Bhawan, MG Marg, Lucknow, Uttar Pradesh-226001, India
Affiliation 2: Academy of Scientific and Innovative Research (AcSIR), CSIR-IITR Campus, 31-Vishvigyan Bhawan, MG Marg, Lucknow, Uttar Pradesh, India
Bio-protocol author page: a4575
, Ruchi Gera
Ruchi GeraAffiliation 1: Immunotoxicology Laboratory, Food Drug and Chemical Toxicology Group and Nanotherapeutics & Nanomaterial Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), 31-Vishvigyan Bhawan, MG Marg, Lucknow, Uttar Pradesh-226001, India
Affiliation 2: Academy of Scientific and Innovative Research (AcSIR), CSIR-IITR Campus, 31-Vishvigyan Bhawan, MG Marg, Lucknow, Uttar Pradesh, India
Bio-protocol author page: a4576
, Mahaveer Prasad Purohit
Mahaveer Prasad PurohitAffiliation 1: Water Analysis Laboratory, Nanotherapeutics and Nanomaterial Toxicology Group, CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, Lucknow-226001, Uttar Pradesh, India
Affiliation 2: Academy of Scientific and Innovative Research (AcSIR), CSIR-IITR Campus, 31-Vishvigyan Bhawan, MG Marg, Lucknow, Uttar Pradesh, India
Bio-protocol author page: a4577
, Satyakam Patnaik
Satyakam PatnaikAffiliation: Water Analysis Laboratory, Nanotherapeutics and Nanomaterial Toxicology Group, CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, Lucknow-226001, Uttar Pradesh, India
Bio-protocol author page: a4578
 and Debabrata Ghosh
Debabrata GhoshAffiliation 1: Immunotoxicology Laboratory, Food Drug and Chemical Toxicology Group and Nanotherapeutics & Nanomaterial Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), 31-Vishvigyan Bhawan, MG Marg, Lucknow, Uttar Pradesh-226001, India
Affiliation 2: Academy of Scientific and Innovative Research (AcSIR), CSIR-IITR Campus, 31-Vishvigyan Bhawan, MG Marg, Lucknow, Uttar Pradesh, India
For correspondence: debabrata.ghosh@iitr.res.in
Bio-protocol author page: a4579
,  date: 6/5/2017, 298 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2304.

[Abstract] Glutathione is one of the major antioxidant defense components present in cells. It is predominantly present as reduced glutathione (GSH) and converted into oxidized glutathione (GSSG) while reducing the free radicals like hydroxyl ions (OH-). For the measurement of GSH and GSSG, o-phthalaldehyde (OPT) ...

Flow Cytometric Analysis of HIV-1 Transcriptional Activity in Response to shRNA Knockdown in A2 and A72 J-Lat Cell Lines

Authors: Daniela Boehm
Daniela BoehmAffiliation: Gladstone Institute of Virology and Immunology, San Francisco, CA, USA
Bio-protocol author page: a4533
 and Melanie Ott
Melanie OttAffiliation 1: Gladstone Institute of Virology and Immunology, San Francisco, CA, USA
Affiliation 2: Department of Medicine, University of California, San Francisco, CA, USA
For correspondence: mott@gladstone.ucsf.edu
Bio-protocol author page: a2100
,  date: 6/5/2017, 320 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2314.

[Abstract] The main obstacle to eradicating HIV-1 from patients is post-integration latency (Finzi et al., 1999). Antiretroviral treatments target only actively replicating virus, while latent infections that have low or no transcriptional activity remain untreated (Sedaghat et al., 2007). To eliminate viral reservoirs, ...

Single-molecule Analysis of DNA Replication Dynamics in Budding Yeast and Human Cells by DNA Combing

Authors: Hélène Tourrière
Hélène TourrièreAffiliation: Institute of Human Genetics, CNRS UMR 9002 and University of Montpellier, Equipe labéllisée LIGUE 2017, Montpellier, France
For correspondence: helene.tourriere@igh.cnrs.fr
Bio-protocol author page: a4580
, Julie Saksouk
Julie SaksoukAffiliation: Institute of Human Genetics, CNRS UMR 9002 and University of Montpellier, Equipe labéllisée LIGUE 2017, Montpellier, France
Bio-protocol author page: a4581
, Armelle Lengronne
Armelle LengronneAffiliation: Institute of Human Genetics, CNRS UMR 9002 and University of Montpellier, Equipe labéllisée LIGUE 2017, Montpellier, France
Bio-protocol author page: a4582
 and Philippe Pasero
Philippe Pasero Affiliation: Institute of Human Genetics, CNRS UMR 9002 and University of Montpellier, Equipe labéllisée LIGUE 2017, Montpellier, France
For correspondence: philippe.pasero@igh.cnrs.fr
Bio-protocol author page: a4583
,  date: 6/5/2017, 364 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2305.

[Abstract] The DNA combing method allows the analysis of DNA replication at the level of individual DNA molecules stretched along silane-coated glass coverslips. Before DNA extraction, ongoing DNA synthesis is labeled with halogenated analogues of thymidine. Replication tracks are visualized by immunofluorescence ...
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Scratch Wound Healing Assay

Author: Yanling Chen
Yanling ChenAffiliation: Department of Immunology, The Scripps Research Institute, La Jolla, USA
For correspondence: ylchen@scripps.edu
Bio-protocol author page: a27
,  date: 3/5/2012, 57536 views, 6 Q&A
DOI: https://doi.org/10.21769/BioProtoc.100.

[Abstract] The scratch wound healing assay has been widely adapted and modified to study the effects of a variety of experimental conditions, for instance, gene knockdown or chemical exposure, on mammalian cell migration and proliferation. In a typical scratch wound healing assay, a “wound gap” in a cell monolayer ...

In vitro Culture of Human PBMCs

Authors: Santosh K Panda
Santosh K PandaAffiliation: Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India
Bio-protocol author page: a221
 and Balachandran Ravindran
Balachandran RavindranAffiliation: Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India
For correspondence: ravindran8@gmail.com
Bio-protocol author page: a222
,  date: 2/5/2013, 45539 views, 4 Q&A
DOI: https://doi.org/10.21769/BioProtoc.322.

[Abstract] Peripheral blood mononuclear cells (PBMCs) consist of chiefly of lymphocytes and monocytes. Purified PBMCs are used in vitro to evaluate a variety of functions of lymphocytes viz; a) proliferation to mitogenic (PHA, Con-A) stimulation, b) monitoring of prior sensitisation in antigen recall assays by ...

Transwell Cell Migration Assay Using Human Breast Epithelial Cancer Cell

Author: Yanling Chen
Yanling ChenAffiliation: Department of Immunology, The Scripps Research Institute, La Jolla, USA
For correspondence: ylchen@scripps.edu
Bio-protocol author page: a27
,  date: 2/20/2012, 44434 views, 7 Q&A
DOI: https://doi.org/10.21769/BioProtoc.99.

[Abstract] Transwell migration assays have been widely used for studying the motility of different types of cells including metastatic cancer cells. The assay is also useful in screens for compounds that act as chemoattractants or inhibitors of chemotaxis for cells. The assay employs a permeable layer of support, ...

Detection of Intracellular Reactive Oxygen Species (CM-H2DCFDA)

Authors: Rabii Ameziane-El-Hassani
Rabii Ameziane-El-HassaniAffiliation 1: UBRM, Centre National de l'Energie, des Sciences et des Techniques Nucléaires, Rabat, Morocco
Affiliation 2: Institut Gustave Roussy, FRE2939 Centre National de la Recherche Scientifique, Villejuif, France
Bio-protocol author page: a200
 and Corinne Dupuy
Corinne DupuyAffiliation 1: Institut Gustave Roussy, FRE2939 Centre National de la Recherche Scientifique, Villejuif, France
Affiliation 2: University Paris, Sud 11, Orsay, France
For correspondence: dupuy@igr.fr
Bio-protocol author page: a201
,  date: 1/5/2013, 43878 views, 2 Q&A
DOI: https://doi.org/10.21769/BioProtoc.313.

[Abstract] Reactive oxygen species (ROS) play a critical role in cellular physiopathology. ROS are implicated in cell proliferation, signaling pathways, oxidative defense mechanisms responsible for killing of bacteria, thyroid hormonosynthesis, etc. The cellular Redox homeostasis is balanced by oxidants and antioxidants ...

Clonogenic Assay

Author: Xiaodong Yang
Xiaodong YangAffiliation: Department of Neurology, University of California, San Francisco, USA
For correspondence: yangxiaodong1@yahoo.com
Bio-protocol author page: a43
,  date: 5/20/2012, 40592 views, 5 Q&A
DOI: https://doi.org/10.21769/BioProtoc.187.

[Abstract] Clonogenic assays serve as a useful tool to test whether a given cancer therapy can reduce the clonogenic survival of tumor cells. A colony is defined as a cluster of at least 50 cells that can often only be determined microscopically. A clonogenic assay is the method of choice to determine cell reproductive ...

In vitro Human Umbilical Vein Endothelial Cells (HUVEC) Tube-formation Assay

Authors: Josephine MY Ko
Josephine MY KoAffiliation: Clinical Oncology Department, The University of Hong Kong, Hong Kong , Hong Kong SAR
Bio-protocol author page: a100
 and Maria Li Lung
Maria Li LungAffiliation: Clinical Oncology Department, The Univerisity of Hong Kong, Hong Kong, Hong Kong SAR
For correspondence: mlilung@hku.hk
Bio-protocol author page: a101
,  date: 9/20/2012, 33978 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.260.

[Abstract] Angiogenesis is involved not only in pathological conditions including cancer biology and non-neoplastic diseases, but also many biological processes including reproduction, development and repair. During angiogenesis, endothelial cells (ECs) undergo activation after binding of angiogenic factors to ...

[Bio101] Cell Adhesion Assay

Author: Yanling Chen
Yanling ChenAffiliation: Department of Immunology, The Scripps Research Institute, La Jolla, USA
For correspondence: ylchen@scripps.edu
Bio-protocol author page: a27
,  date: 3/5/2012, 30222 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.98.

[Abstract] Cell adhesion, the binding of a cell to the extracellular matrix (ECM), other cells, or a specific surface, is essential for the growth and survival of the cell and also its communication with other cells. The process of cell adhesion involves a range of biological events such as three-dimensional re-organization ...

[Bio101] Calcium Phosphate Transfection of Eukaryotic Cells

Author: Yanling Chen
Yanling ChenAffiliation: Department of Immunology, The Scripps Research Institute, La Jolla, USA
For correspondence: ylchen@scripps.edu
Bio-protocol author page: a27
,  date: 2/5/2012, 28873 views, 2 Q&A
DOI: https://doi.org/10.21769/BioProtoc.86.

[Abstract] Transfection of DNA into cells is an indispensible protocol in molecular biology. While plenty of lipid-based transfection reagents are commercially available nowadays, a quick, simple, efficient and inexpensive method is to transfect eukaryotic cells via calcium phosphate co-precipitation with DNA ...

In vitro Tumorsphere Formation Assays

Authors: Sara Johnson
Sara JohnsonAffiliation: Biological Sciences Department, University of South Carolina, Columbia, USA
Bio-protocol author page: a224
, Hexin Chen
Hexin ChenAffiliation: Biological Sciences Department, University of South Carolina, Columbia, USA
For correspondence: hchen@biol.sc.edu
Bio-protocol author page: a225
 and Pang-Kuo Lo
Pang-Kuo LoAffiliation: Biological Sciences Department, University of South Carolina, Columbia, USA
Bio-protocol author page: a226
,  date: 2/5/2013, 25894 views, 3 Q&A
DOI: https://doi.org/10.21769/BioProtoc.325.

[Abstract] A tumorsphere is a solid, spherical formation developed from the proliferation of one cancer stem/progenitor cell. These tumorspheres (Figure 1a) are easily distinguishable from single or aggregated cells (Figure 1b) as the cells appear to become fused together and individual cells cannot be identified. ...

Analysis of Protein Stability by the Cycloheximide Chase Assay

Authors: Shih-Han Kao
Shih-Han KaoAffiliation: Research Center for Tumor Medical Science, China Medical University, Taichung, Taiwan
Bio-protocol author page: a1920
, Wen-Lung Wang
Wen-Lung WangAffiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
Bio-protocol author page: a1921
, Chi-Yuan Chen
Chi-Yuan ChenAffiliation: Department of Nutrition and Health Sciences, Chang Gung University of Science and Technology, Taoyuan, Taiwan
Bio-protocol author page: a1922
, Yih-Leong Chang
Yih-Leong ChangAffiliation: Department of Pathology and Graduate Institute of Pathology, National Taiwan University, Taipei, Taiwan
Bio-protocol author page: a1923
, Yi-Ying Wu
Yi-Ying WuAffiliation: Graduate Institute of Clinical Medicine, National Cheng Kung University, Taipei, Taiwan
Bio-protocol author page: a1924
, Yi-Ting Wang
Yi-Ting WangAffiliation 1: Chemical Biology and Molecular Biophysics Program, Academia Sinica, Taipei, Taiwan
Affiliation 2: Institute of Chemistry, Academia Sinica, Taipei, Taiwan
Affiliation 3: Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan
Bio-protocol author page: a1925
, Shu-Ping Wang
Shu-Ping WangAffiliation: Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, USA
Bio-protocol author page: a1926
, Alexey I Nesvizhskii
Alexey I NesvizhskiiAffiliation 1: Department of Pathology, University of Michigan, Ann Arbor, USA
Affiliation 2: Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, USA
Bio-protocol author page: a1927
, Yu-Ju Chen
Yu-Ju ChenAffiliation 1: Chemical Biology and Molecular Biophysics Program, Academia Sinica, Taipei, Taiwan
Affiliation 2: Institute of Chemistry, Academia Sinica, Taipei, Taiwan
Bio-protocol author page: a1928
, Tse-Ming Hong
Tse-Ming HongAffiliation: Graduate Institute of Clinical Medicine, National Cheng Kung University, Taipei, Taiwan
Bio-protocol author page: a1929
 and Pan-Chyr Yang
Pan-Chyr YangAffiliation 1: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
Affiliation 2: Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan
Affiliation 3: NTU Center of Genomic Medicine, National Taiwan University, Taipei, Taiwan
Present address: Department of Internal Medicine, National Taiwan University College of Medicine, Taipei, Taiwan
For correspondence: pcyang@ntu.edu.tw
Bio-protocol author page: a574
,  date: 1/5/2015, 24053 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1374.

[Abstract] Comparison of protein stability in eukaryotic cells has been achieved by cycloheximide, which is an inhibitor of protein biosynthesis due to its prevention in translational elongation. It is broadly used in cell biology in terms of determining the half-life of a given protein and has gained much popularity ...
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Mammalia
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