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Functional ex-vivo Imaging of Arterial Cellular Recruitment and Lipid Extravasation

Authors: Emiel P.C. van der Vorst
Emiel P.C. van der VorstAffiliation: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
For correspondence: Emiel.Van_der_Vorst@med.uni-muenchen.de
Bio-protocol author page: a4681
, Sanne L. Maas
Sanne L. MaasAffiliation: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Bio-protocol author page: a4682
, Almudena Ortega-Gomez
Almudena Ortega-GomezAffiliation: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Bio-protocol author page: a4683
, Jeroen M.M. Hameleers
Jeroen M.M. HameleersAffiliation: Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands
Bio-protocol author page: a4684
, Mariaelvy Bianchini
Mariaelvy BianchiniAffiliation: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Bio-protocol author page: a4685
, Yaw Asare
Yaw AsareAffiliation: Institute for Stroke and Dementia Research, Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Bio-protocol author page: a4686
, Oliver Soehnlein
Oliver SoehnleinAffiliation 1: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Affiliation 2: DZHK (German Centre for Cardiovascular Research), partner site Munich Heart Alliance, Munich, Germany
Affiliation 3: Department of Physiology and Pharmacology (FyFa), Karolinska Institutet, Stockholm, Sweden
Bio-protocol author page: a4687
, Yvonne Döring
Yvonne DöringAffiliation: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Bio-protocol author page: a4688
, Christian Weber
Christian WeberAffiliation 1: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Affiliation 2: Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands
Affiliation 3: DZHK (German Centre for Cardiovascular Research), partner site Munich Heart Alliance, Munich, Germany
Bio-protocol author page: a3209
 and Remco T.A. Megens
Remco T.A. MegensAffiliation 1: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Affiliation 2: Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands
For correspondence: Remco.Megens@med.uni-muenchen.de
Bio-protocol author page: a4689
,  date: 6/20/2017, 269 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2344.

[Abstract] The main purpose of this sophisticated and highly versatile method is to visualize and quantify structural vessel wall properties, cellular recruitment, and lipid/dextran extravasation under physiological conditions in living arteries. This will be of interest for a broad range of researchers within ...

Vascular Smooth Muscle Cell Isolation and Culture from Mouse Aorta

Authors: Callie S. Kwartler
Callie S. KwartlerAffiliation: Department of Internal Medicine, Division of Medical Genetics, University of Texas Health Science Center, Houston, Texas, USA
Bio-protocol author page: a3821
, Ping Zhou
Ping ZhouAffiliation: Department of Internal Medicine, Division of Medical Genetics, University of Texas Health Science Center, Houston, Texas, USA
Bio-protocol author page: a3822
, Shao-Qing Kuang
Shao-Qing KuangAffiliation: Department of Internal Medicine, Division of Medical Genetics, University of Texas Health Science Center, Houston, Texas, USA
Bio-protocol author page: a3823
, Xue-Yan Duan
Xue-Yan DuanAffiliation: Department of Internal Medicine, Division of Medical Genetics, University of Texas Health Science Center, Houston, Texas, USA
Bio-protocol author page: a3824
, Limin Gong
Limin GongAffiliation: Department of Internal Medicine, Division of Medical Genetics, University of Texas Health Science Center, Houston, Texas, USA
Bio-protocol author page: a3825
 and Dianna M. Milewicz
Dianna M. MilewiczAffiliation: Department of Internal Medicine, Division of Medical Genetics, University of Texas Health Science Center, Houston, Texas, USA
For correspondence: Dianna.M.Milewicz@uth.tmc.edu
Bio-protocol author page: a3827
,  date: 12/5/2016, 1685 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2045.

[Abstract] Vascular smooth muscle cells (SMC) in the ascending thoracic aorta arise from neural crest cells, whereas SMCs in the descending aorta are derived from the presomitic mesoderm. SMCs play important roles in cardiovascular development and aortic aneurysm formation. This protocol describes the detailed ...

Aorta Ring Assay

Authors: Jing Jin
Jing JinAffiliation 1: Second Affiliated hospital Zhejiang University college of Medicine of clinic research center, Hangzhou, China
Affiliation 2: Cardiovascular key laboratory of Zhejiang province, Hangzhou, China
Bio-protocol author page: a3272
, Xinyang Hu
Xinyang HuAffiliation 1: Second Affiliated hospital Zhejiang University college of Medicine of clinic research center, Hangzhou, China
Affiliation 2: Second Affiliated hospital Zhejiang University college of Medicine of Department of science and education, Hangzhou, China
Bio-protocol author page: a3273
, Ling Zhang
Ling ZhangAffiliation: Cardiovascular key laboratory of Zhejiang province, Hangzhou, China
Bio-protocol author page: a3367
 and Jian'an Wang
Jian'an WangAffiliation 1: Second Affiliated hospital Zhejiang University college of Medicine of clinic research center, Hangzhou, China
Affiliation 2: Cardiovascular key laboratory of Zhejiang province, Hangzhou, China
For correspondence: jian_an_wang@yahoo.com
Bio-protocol author page: a3368
,  date: 7/5/2016, 1537 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1856.

[Abstract] Angiogenesis is the nature and the pathological process of blood vessels growth from pre-existing vascular bud. It plays an important role in cancer and cardiovascular disease. Aorta Ring Assay is an approach of Angiogenesis. In this experiment, we use the aorta of rat as material, clean surrounding ...

Preparation of Single Cell Suspensions from Mouse Aorta

Authors: Desheng Hu
Desheng HuAffiliation 1: Institute for Immunology, Ludwig-Maximilians-University Munich, Munich, Germany
Affiliation 2: Institute of Molecular Immunology, Helmholtz Zentrum Munich, Munich, Germany
For correspondence: desheng.hu@med.uni-muenchen.de
Bio-protocol author page: a3210
, Changjun Yin
Changjun YinAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University Munich, Munich, Germany
Bio-protocol author page: a3208
, Sarajo Mohanta
Sarajo MohantaAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University Munich, Munich, Germany
Bio-protocol author page: a3207
, Christian Weber
Christian WeberAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University Munich, Munich, Germany
Bio-protocol author page: a3209
 and Andreas J. R. Habenicht
Andreas J. R. HabenichtAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University Munich, Munich, Germany
Bio-protocol author page: a3211
,  date: 6/5/2016, 2206 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1832.

[Abstract] Atherosclerosis is a chronic inflammatory disease of the arterial wall characterized by lipid deposition, plaque formation, and immune cell infiltration. Innate and adaptive immune cells infiltrate the artery during development of the disease. Moreover, advanced disease leads to formation of artery ...

Aorta Atherosclerosis Lesion Analysis in Hyperlipidemic Mice

Authors: Sarajo Mohanta
Sarajo MohantaAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Pettenkoferstrasse, Munich, Germany
For correspondence: Sarajo.Mohanta@med.uni-muenchen.de
Bio-protocol author page: a3207
, Changjun Yin
Changjun YinAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Pettenkoferstrasse, Munich, Germany
Bio-protocol author page: a3208
, Christian Weber
Christian WeberAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Pettenkoferstrasse, Munich, Germany
Bio-protocol author page: a3209
, Desheng Hu
Desheng HuAffiliation 1: Institute of Molecular Immunology, Helmholtz Zentrum München, Marchioninistrasse, Munich, Germany
Affiliation 2: Institute for Immunology, Ludwig-Maximilians-University, Großhadernerstrasse, Munich, Germany
Bio-protocol author page: a3210
 and Andreas J. R. Habenicht
Andreas J. R. HabenichtAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Pettenkoferstrasse, Munich, Germany
Bio-protocol author page: a3211
,  date: 6/5/2016, 2638 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1833.

[Abstract] Atherosclerosis is a chronic inflammatory disease of large and medium-sized arteries. Apolipoprotein E-deficient (ApoE-/-) mice are used as experimental models to study human atherosclerosis. ApoE-/- mice are constitutively hyperlipidemic and develop intima plaques that resemble human plaques. Various ...

Transfection of Embryoid Bodies with miRNA Precursors to Induce Cardiac Differentiation

Authors: Federica Pisano
Federica PisanoAffiliation 1: Department of Cardiothoracic and Vascular Sciences-Coronary Care Unit and Laboratory of Clinical and Experimental Cardiology-Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
Affiliation 2: Laboratory of Experimental Cardiology for Cell and Molecular Therapy, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
Bio-protocol author page: a2897
 and Massimiliano Gnecchi
Massimiliano GnecchiAffiliation 1: Department of Cardiothoracic and Vascular Sciences-Coronary Care Unit and Laboratory of Clinical and Experimental Cardiology-Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
Affiliation 2: Laboratory of Experimental Cardiology for Cell and Molecular Therapy, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
Affiliation 3: Department of Molecular Medicine-Unit of Cardiology-University of Pavia, Pavia, Italy
Affiliation 4: Department of Medicine-University of Cape Town-Cape Town, South Africa
For correspondence: m.gnecchi@unipv.it
Bio-protocol author page: a2898
,  date: 2/5/2016, 1672 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1726.

[Abstract] In recent years, the utilization of stem cell therapy to regenerate cardiac tissue has been proposed as a possible strategy to treat cardiac damage (Gnecchi et al., 2012, Aguirre et al., 2013; Sanganalmath and Bolli, 2013). Although encouraging results have been obtained in experimental models, the ...

Permanent Occlusion of the Left Anterior Coronary Artery in the Rat

Authors: Mélanie Bousquenaud
Mélanie BousquenaudAffiliation: Laboratory of Pathology, Dept of Medicine, University of Fribourg, Fribourg, Switzerland
For correspondence: melanie.bousquenaud@unifr.ch
Bio-protocol author page: a2737
, Pierre-Yves Marie
Pierre-Yves MarieAffiliation: Nancyclotep, Experimental Imaging Platform, Nancy, France
Bio-protocol author page: a2738
 and Yvan Devaux
Yvan DevauxAffiliation: Laboratory of Cardiovascular Research, Luxembourg Institut of Health, Luxembourg, Luxembourg
Bio-protocol author page: a2642
,  date: 11/20/2015, 1366 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1663.

[Abstract] Left ventricular (LV) remodeling occurs in many patients after myocardial infarction (MI). LV remodeling is characterized by progressive ventricular dilatation and contractile dysfunction, consequently to cardiomyocyte hypertrophy and fibrosis. Despite reperfusion therapies, this pathophysiological ...

Rat Aortic Ring Model to Assay Angiogenesis ex vivo

Authors: Isabelle Ernens
Isabelle ErnensAffiliation: Laboratory of Cardiovascular Research, Luxembourg Institute of Health (L.I.H.), Luxembourg, Luxembourg
For correspondence: isabelle.ernens@lih.lu
Bio-protocol author page: a2640
, Bénédicte Lenoir
Bénédicte LenoirAffiliation: Laboratory of Cardiovascular Research, Luxembourg Institute of Health (L.I.H.), Luxembourg, Luxembourg
Bio-protocol author page: a2641
, Yvan Devaux
Yvan DevauxAffiliation: Laboratory of Cardiovascular Research, Luxembourg Institute of Health (L.I.H.), Luxembourg, Luxembourg
Bio-protocol author page: a2642
 and Daniel R. Wagner
Daniel R. WagnerAffiliation: Division of Cardiology, Centre Hospitalier, Luxembourg, Luxembourg
Bio-protocol author page: a2643
,  date: 10/20/2015, 2152 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1622.

[Abstract] Angiogenesis is a multifactorial event which requires the migration, proliferation, differentiation and structure rearrangement of endothelial cells. This angiogenic process has been commonly studied using in vitro assays such as Boyden chamber assay, wound healing assay and tube formation assay. These ...

Mouse Retinal Whole Mounts and Quantification of Vasculature Protocol

Authors: Irit Adini
Irit AdiniAffiliation: Department of Bioengineering, Program in Cell, Molecular and Developmental Biology, University of California, Riverside, USA
For correspondence: irit.adini@gmail.com
Bio-protocol author page: a2415
 and Kaustabh Ghosh
Kaustabh GhoshAffiliation: Department of Bioengineering, Program in Cell, Molecular and Developmental Biology, University of California, Riverside, USA
Bio-protocol author page: a2416
,  date: 8/5/2015, 3144 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1546.

[Abstract] Angiogenesis is the formation of new blood vessels from a pre-existing vascular bed. It is a multi-step process beginning with enzymatic degradation of the capillary basement membrane, followed by endothelial cell (EC) proliferation, migration, tube formation, assembly of a new basement membrane, and ...

Monocular Deprivation in Mice

Authors: Ya-tang Li
Ya-tang LiAffiliation: Zilkha Neurogenetic Institute, University of Southern California, Los Angeles, USA
For correspondence: yatangli8@gmail.com
Bio-protocol author page: a1109
, Xiao-lin Chou
Xiao-lin ChouAffiliation: Zilkha Neurogenetic Institute, University of Southern California, Los Angeles, USA
Bio-protocol author page: a1131
 and Huizhong Whit Tao
Huizhong Whit Tao Affiliation: Zilkha Neurogenetic Institute, University of Southern California, Los Angeles, USA
Bio-protocol author page: a1132
,  date: 1/20/2014, 3296 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1024.

[Abstract] Monocular deprivation is an experimental technique to study the ocular dominance plasticity during critical period (Hubel and Wiesel, 1963). Generally one eye of an animal is sutured during critical period, and the sutured eye is re-opened after either less than three days (short term) or more than ...
1 2 

Assay of Blood Brain Barrier and Placental Barrier Permeability

Authors: Saurabh Kumar Agnihotri*
Saurabh Kumar AgnihotriAffiliation: Endocrinology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Bio-protocol author page: a726
, Poonam Singh*
Poonam SinghAffiliation: Toxicology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Bio-protocol author page: a727
, Balawant Kumar
Balawant KumarAffiliation: Toxicology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Bio-protocol author page: a728
, Pankaj Singh
Pankaj SinghAffiliation: Endocrinology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Bio-protocol author page: a729
, Swatantra Kumar Jain
Swatantra Kumar JainAffiliation: Endocrinology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Bio-protocol author page: a730
, Mahesh Chandra Tewari
Mahesh Chandra TewariAffiliation: Endocrinology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Bio-protocol author page: a731
, Sadan Kumar
Sadan KumarAffiliation: Toxicology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Bio-protocol author page: a732
, Monika Sachdev
Monika SachdevAffiliation: Endocrinology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
For correspondence: smonika26@yahoo.com
Bio-protocol author page: a400
 and Raj Kamal Tripathi
Raj Kamal TripathiAffiliation: Toxicology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
For correspondence: rk_tripathi@cdri.res.in
Bio-protocol author page: a734
 (*contributed equally to this work),  date: 8/5/2013, 3955 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.845.

[Abstract] Evans blue dye solution was used to observe the effect of a viral protein on two pre-defined barriers of the body i.e. the blood brain barrier (BBB) and the placental barrier (PB). This dye has strong affinity for serum albumin and does not cross these barriers under natural conditions. As all the dye ...

Monocular Deprivation in Mice

Authors: Ya-tang Li
Ya-tang LiAffiliation: Zilkha Neurogenetic Institute, University of Southern California, Los Angeles, USA
For correspondence: yatangli8@gmail.com
Bio-protocol author page: a1109
, Xiao-lin Chou
Xiao-lin ChouAffiliation: Zilkha Neurogenetic Institute, University of Southern California, Los Angeles, USA
Bio-protocol author page: a1131
 and Huizhong Whit Tao
Huizhong Whit Tao Affiliation: Zilkha Neurogenetic Institute, University of Southern California, Los Angeles, USA
Bio-protocol author page: a1132
,  date: 1/20/2014, 3296 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1024.

[Abstract] Monocular deprivation is an experimental technique to study the ocular dominance plasticity during critical period (Hubel and Wiesel, 1963). Generally one eye of an animal is sutured during critical period, and the sutured eye is re-opened after either less than three days (short term) or more than ...

Mouse Retinal Whole Mounts and Quantification of Vasculature Protocol

Authors: Irit Adini
Irit AdiniAffiliation: Department of Bioengineering, Program in Cell, Molecular and Developmental Biology, University of California, Riverside, USA
For correspondence: irit.adini@gmail.com
Bio-protocol author page: a2415
 and Kaustabh Ghosh
Kaustabh GhoshAffiliation: Department of Bioengineering, Program in Cell, Molecular and Developmental Biology, University of California, Riverside, USA
Bio-protocol author page: a2416
,  date: 8/5/2015, 3144 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1546.

[Abstract] Angiogenesis is the formation of new blood vessels from a pre-existing vascular bed. It is a multi-step process beginning with enzymatic degradation of the capillary basement membrane, followed by endothelial cell (EC) proliferation, migration, tube formation, assembly of a new basement membrane, and ...

Aorta Atherosclerosis Lesion Analysis in Hyperlipidemic Mice

Authors: Sarajo Mohanta
Sarajo MohantaAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Pettenkoferstrasse, Munich, Germany
For correspondence: Sarajo.Mohanta@med.uni-muenchen.de
Bio-protocol author page: a3207
, Changjun Yin
Changjun YinAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Pettenkoferstrasse, Munich, Germany
Bio-protocol author page: a3208
, Christian Weber
Christian WeberAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Pettenkoferstrasse, Munich, Germany
Bio-protocol author page: a3209
, Desheng Hu
Desheng HuAffiliation 1: Institute of Molecular Immunology, Helmholtz Zentrum München, Marchioninistrasse, Munich, Germany
Affiliation 2: Institute for Immunology, Ludwig-Maximilians-University, Großhadernerstrasse, Munich, Germany
Bio-protocol author page: a3210
 and Andreas J. R. Habenicht
Andreas J. R. HabenichtAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Pettenkoferstrasse, Munich, Germany
Bio-protocol author page: a3211
,  date: 6/5/2016, 2638 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1833.

[Abstract] Atherosclerosis is a chronic inflammatory disease of large and medium-sized arteries. Apolipoprotein E-deficient (ApoE-/-) mice are used as experimental models to study human atherosclerosis. ApoE-/- mice are constitutively hyperlipidemic and develop intima plaques that resemble human plaques. Various ...

Preparation of Single Cell Suspensions from Mouse Aorta

Authors: Desheng Hu
Desheng HuAffiliation 1: Institute for Immunology, Ludwig-Maximilians-University Munich, Munich, Germany
Affiliation 2: Institute of Molecular Immunology, Helmholtz Zentrum Munich, Munich, Germany
For correspondence: desheng.hu@med.uni-muenchen.de
Bio-protocol author page: a3210
, Changjun Yin
Changjun YinAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University Munich, Munich, Germany
Bio-protocol author page: a3208
, Sarajo Mohanta
Sarajo MohantaAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University Munich, Munich, Germany
Bio-protocol author page: a3207
, Christian Weber
Christian WeberAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University Munich, Munich, Germany
Bio-protocol author page: a3209
 and Andreas J. R. Habenicht
Andreas J. R. HabenichtAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University Munich, Munich, Germany
Bio-protocol author page: a3211
,  date: 6/5/2016, 2206 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1832.

[Abstract] Atherosclerosis is a chronic inflammatory disease of the arterial wall characterized by lipid deposition, plaque formation, and immune cell infiltration. Innate and adaptive immune cells infiltrate the artery during development of the disease. Moreover, advanced disease leads to formation of artery ...

Rat Aortic Ring Model to Assay Angiogenesis ex vivo

Authors: Isabelle Ernens
Isabelle ErnensAffiliation: Laboratory of Cardiovascular Research, Luxembourg Institute of Health (L.I.H.), Luxembourg, Luxembourg
For correspondence: isabelle.ernens@lih.lu
Bio-protocol author page: a2640
, Bénédicte Lenoir
Bénédicte LenoirAffiliation: Laboratory of Cardiovascular Research, Luxembourg Institute of Health (L.I.H.), Luxembourg, Luxembourg
Bio-protocol author page: a2641
, Yvan Devaux
Yvan DevauxAffiliation: Laboratory of Cardiovascular Research, Luxembourg Institute of Health (L.I.H.), Luxembourg, Luxembourg
Bio-protocol author page: a2642
 and Daniel R. Wagner
Daniel R. WagnerAffiliation: Division of Cardiology, Centre Hospitalier, Luxembourg, Luxembourg
Bio-protocol author page: a2643
,  date: 10/20/2015, 2152 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1622.

[Abstract] Angiogenesis is a multifactorial event which requires the migration, proliferation, differentiation and structure rearrangement of endothelial cells. This angiogenic process has been commonly studied using in vitro assays such as Boyden chamber assay, wound healing assay and tube formation assay. These ...

Vascular Smooth Muscle Cell Isolation and Culture from Mouse Aorta

Authors: Callie S. Kwartler
Callie S. KwartlerAffiliation: Department of Internal Medicine, Division of Medical Genetics, University of Texas Health Science Center, Houston, Texas, USA
Bio-protocol author page: a3821
, Ping Zhou
Ping ZhouAffiliation: Department of Internal Medicine, Division of Medical Genetics, University of Texas Health Science Center, Houston, Texas, USA
Bio-protocol author page: a3822
, Shao-Qing Kuang
Shao-Qing KuangAffiliation: Department of Internal Medicine, Division of Medical Genetics, University of Texas Health Science Center, Houston, Texas, USA
Bio-protocol author page: a3823
, Xue-Yan Duan
Xue-Yan DuanAffiliation: Department of Internal Medicine, Division of Medical Genetics, University of Texas Health Science Center, Houston, Texas, USA
Bio-protocol author page: a3824
, Limin Gong
Limin GongAffiliation: Department of Internal Medicine, Division of Medical Genetics, University of Texas Health Science Center, Houston, Texas, USA
Bio-protocol author page: a3825
 and Dianna M. Milewicz
Dianna M. MilewiczAffiliation: Department of Internal Medicine, Division of Medical Genetics, University of Texas Health Science Center, Houston, Texas, USA
For correspondence: Dianna.M.Milewicz@uth.tmc.edu
Bio-protocol author page: a3827
,  date: 12/5/2016, 1685 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2045.

[Abstract] Vascular smooth muscle cells (SMC) in the ascending thoracic aorta arise from neural crest cells, whereas SMCs in the descending aorta are derived from the presomitic mesoderm. SMCs play important roles in cardiovascular development and aortic aneurysm formation. This protocol describes the detailed ...

Transfection of Embryoid Bodies with miRNA Precursors to Induce Cardiac Differentiation

Authors: Federica Pisano
Federica PisanoAffiliation 1: Department of Cardiothoracic and Vascular Sciences-Coronary Care Unit and Laboratory of Clinical and Experimental Cardiology-Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
Affiliation 2: Laboratory of Experimental Cardiology for Cell and Molecular Therapy, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
Bio-protocol author page: a2897
 and Massimiliano Gnecchi
Massimiliano GnecchiAffiliation 1: Department of Cardiothoracic and Vascular Sciences-Coronary Care Unit and Laboratory of Clinical and Experimental Cardiology-Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
Affiliation 2: Laboratory of Experimental Cardiology for Cell and Molecular Therapy, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
Affiliation 3: Department of Molecular Medicine-Unit of Cardiology-University of Pavia, Pavia, Italy
Affiliation 4: Department of Medicine-University of Cape Town-Cape Town, South Africa
For correspondence: m.gnecchi@unipv.it
Bio-protocol author page: a2898
,  date: 2/5/2016, 1672 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1726.

[Abstract] In recent years, the utilization of stem cell therapy to regenerate cardiac tissue has been proposed as a possible strategy to treat cardiac damage (Gnecchi et al., 2012, Aguirre et al., 2013; Sanganalmath and Bolli, 2013). Although encouraging results have been obtained in experimental models, the ...

Aorta Ring Assay

Authors: Jing Jin
Jing JinAffiliation 1: Second Affiliated hospital Zhejiang University college of Medicine of clinic research center, Hangzhou, China
Affiliation 2: Cardiovascular key laboratory of Zhejiang province, Hangzhou, China
Bio-protocol author page: a3272
, Xinyang Hu
Xinyang HuAffiliation 1: Second Affiliated hospital Zhejiang University college of Medicine of clinic research center, Hangzhou, China
Affiliation 2: Second Affiliated hospital Zhejiang University college of Medicine of Department of science and education, Hangzhou, China
Bio-protocol author page: a3273
, Ling Zhang
Ling ZhangAffiliation: Cardiovascular key laboratory of Zhejiang province, Hangzhou, China
Bio-protocol author page: a3367
 and Jian'an Wang
Jian'an WangAffiliation 1: Second Affiliated hospital Zhejiang University college of Medicine of clinic research center, Hangzhou, China
Affiliation 2: Cardiovascular key laboratory of Zhejiang province, Hangzhou, China
For correspondence: jian_an_wang@yahoo.com
Bio-protocol author page: a3368
,  date: 7/5/2016, 1537 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1856.

[Abstract] Angiogenesis is the nature and the pathological process of blood vessels growth from pre-existing vascular bud. It plays an important role in cancer and cardiovascular disease. Aorta Ring Assay is an approach of Angiogenesis. In this experiment, we use the aorta of rat as material, clean surrounding ...

Permanent Occlusion of the Left Anterior Coronary Artery in the Rat

Authors: Mélanie Bousquenaud
Mélanie BousquenaudAffiliation: Laboratory of Pathology, Dept of Medicine, University of Fribourg, Fribourg, Switzerland
For correspondence: melanie.bousquenaud@unifr.ch
Bio-protocol author page: a2737
, Pierre-Yves Marie
Pierre-Yves MarieAffiliation: Nancyclotep, Experimental Imaging Platform, Nancy, France
Bio-protocol author page: a2738
 and Yvan Devaux
Yvan DevauxAffiliation: Laboratory of Cardiovascular Research, Luxembourg Institut of Health, Luxembourg, Luxembourg
Bio-protocol author page: a2642
,  date: 11/20/2015, 1366 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1663.

[Abstract] Left ventricular (LV) remodeling occurs in many patients after myocardial infarction (MI). LV remodeling is characterized by progressive ventricular dilatation and contractile dysfunction, consequently to cardiomyocyte hypertrophy and fibrosis. Despite reperfusion therapies, this pathophysiological ...
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