Bacteria

Determination of (p)ppGpp Levels During Stringent Response in Streptomyces coelicolor by Thin Layer Chromatography

Authors: Smitha Sivapragasam
Smitha SivapragasamAffiliation: Department of Biological Sciences, Louisiana State University, Baton Rouge, USA
For correspondence: ssivap1@lsu.edu
Bio-protocol author page: a3679
and Anne Grove
Anne GroveAffiliation: Department of Biological Sciences, Louisiana State University, Baton Rouge, USA
Bio-protocol author page: a3680
, date: 11/5/2016, 1302 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1995.

[Abstract] The stringent response in bacteria is a stress response that is mediated by the signaling molecules guanosine tetraphosphate and pentaphosphate [(p)ppGpp], alarmones that are also directly related to virulence. Therefore, determination of (p)ppGpp levels is crucial for studying the stringent response. ...

Determination of Oxygen Respiration Rates in Wetted Developmentally Arrested Spores of Streptomyces Species

Authors: Marco Fischer
Marco FischerAffiliation: Department of Microbiology, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany
Bio-protocol author page: a1422
, Dörte Falke
Dörte FalkeAffiliation: Department of Microbiology, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany
Bio-protocol author page: a1423
and R. Gary Sawers
R. Gary SawersAffiliation: Department of Microbiology, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany
For correspondence: gary.sawers@mikrobiologie.uni-halle.de
Bio-protocol author page: a1424
, date: 6/20/2014, 2961 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1153.

[Abstract] Streptomyces species produce spores, which, while not as robust as endospores of Bacillus or Clostridium species, are capable of surviving for months or even years (Hopwood, 2006). During this time these spores remain viable, surviving by slowly degrading internal stores of carbon compounds, such as ...

Rapid Nitrate Reduction Assay with Intact Microbial Cells or Spores

Authors: Marco Fischer
Marco FischerAffiliation: Department of Microbiology, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany
Bio-protocol author page: a1422
, Dörte Falke
Dörte FalkeAffiliation: Department of Microbiology, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany
Bio-protocol author page: a1423
and R. Gary Sawers
R. Gary SawersAffiliation: Department of Microbiology, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany
For correspondence: gary.sawers@mikrobiologie.uni-halle.de
Bio-protocol author page: a1424
, date: 6/20/2014, 2851 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1154.

[Abstract] Many microorganisms have the capacity to use nitrate as a respiratory electron acceptor. Reduction of nitrate is catalyzed by a multi-subunit nitrate reductase that is often located associated with the cytoplasmic membrane and has its active site oriented toward the cytoplasm. This means that nitrate ...

	Minimal Inhibitory Concentration (MIC) Assay for Acinetobacter baumannii
	Colony Immunoblotting Assay for Detection of Bacterial Cell-surface or Extracellular Proteins
	Measurement of Chlorophyll a and Carotenoids Concentration in Cyanobacteria
	Pyocyanin Extraction and Quantitative Analysis in Swarming Pseudomonas aeruginosa
	Gentamicin Protection Assay to Determine Bacterial Survival within Macrophages
	RNA Isolation from Synechocystis
	Isolation of Rhizosphere Bacterial Communities from Soil
	In vitro RNA-protein Binding Assay by UV Crosslinking
	Protein-peptide Interaction by Surface Plasmon Resonance
	Heterologous Expression and Purification of the Magnesium Transporter A (MgtA) in Escherichia coli