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Single-step Marker Switching in
Schizosaccharomyces pombe
Using a Lithium Acetate Transformation Protocol
Authors:
Simon David Brown
Simon David Brown
Affiliation:
Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK
Bio-protocol author page:
a3916
and
Alexander Lorenz
Alexander Lorenz
Affiliation:
Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK
For correspondence:
a.lorenz@abdn.ac.uk
Bio-protocol author page:
a3917
,
date: 12/20/2016, 1321 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.2075
.
[Abstract] The ability to utilize different selectable markers for tagging or mutating multiple genes in
Schizosaccharomyces pombe
is hampered by the historical use of only two selectable markers,
ura4
+
and
kanMX6
; the latter conferring resistance to the antibiotic G418 (geneticin). More markers have been described ...
Chromatin Fractionation Assay in Fission Yeast
Authors:
Tatsuki Kunoh
Tatsuki Kunoh
Affiliation:
Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan
For correspondence:
tkunoh06@gmail.com
Bio-protocol author page:
a1508
and
Toshiyuki Habu
Toshiyuki Habu
Affiliation:
Radiation Biology Center, Kyoto University, Kyoto, Japan
Bio-protocol author page:
a1509
,
date: 7/20/2014, 3144 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1185
.
[Abstract] The protein recruitment onto chromatin is a critical process for DNA metabolism, including DNA replication, DNA repair and DNA recombination. Especially DNA modification enzymes and checkpoint proteins are loaded onto DNA damage sites in a context-dependent manner. In our recent study (Kunoh and Habu, ...
Fluorescence Recovery After Photobleaching (FRAP) in the Fission Yeast Nucleus
Authors:
Petrina Delivani
Petrina Delivani
Affiliation:
Department of Molecular Cell Biology and Genetics, Max Planck Institute, Dresden, Germany
For correspondence:
delivani@mpi-cbg.de
Bio-protocol author page:
a914
,
Mariola R. Chacón
Mariola R. Chacón
Affiliation:
Department of Molecular Cell Biology and Genetics, Max Planck Institute, Dresden, Germany
For correspondence:
mchacon@mpi-cbg.de
Bio-protocol author page:
a915
,
Britta Schroth-Diez
Britta Schroth-Diez
Affiliation:
Department of Molecular Cell Biology and Genetics, Max Planck Institute, Dresden, Germany
For correspondence:
schroth@mpi-cbg.de
Bio-protocol author page:
a916
and
Iva M. Tolić-Nørrelykke
Iva M. Tolić-Nørrelykke
Affiliation:
Department of Molecular Cell Biology and Genetics, Max Planck Institute, Dresden, Germany
For correspondence:
tolic@mpi-cbg.de
Bio-protocol author page:
a493
,
date: 10/20/2013, 3864 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.941
.
[Abstract] We use fluorescence recovery after photobleaching (FRAP) to calculate the diffusion coefficient of GFP in the nucleoplasm of fission yeast. The FRAP method can be generally used to measure the mobility of proteins inside the cell or its organelles.
In our experiment we only measured the diffusion of ...
1
Fluorescence Recovery After Photobleaching (FRAP) in the Fission Yeast Nucleus
Authors:
Petrina Delivani
Petrina Delivani
Affiliation:
Department of Molecular Cell Biology and Genetics, Max Planck Institute, Dresden, Germany
For correspondence:
delivani@mpi-cbg.de
Bio-protocol author page:
a914
,
Mariola R. Chacón
Mariola R. Chacón
Affiliation:
Department of Molecular Cell Biology and Genetics, Max Planck Institute, Dresden, Germany
For correspondence:
mchacon@mpi-cbg.de
Bio-protocol author page:
a915
,
Britta Schroth-Diez
Britta Schroth-Diez
Affiliation:
Department of Molecular Cell Biology and Genetics, Max Planck Institute, Dresden, Germany
For correspondence:
schroth@mpi-cbg.de
Bio-protocol author page:
a916
and
Iva M. Tolić-Nørrelykke
Iva M. Tolić-Nørrelykke
Affiliation:
Department of Molecular Cell Biology and Genetics, Max Planck Institute, Dresden, Germany
For correspondence:
tolic@mpi-cbg.de
Bio-protocol author page:
a493
,
date: 10/20/2013, 3864 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.941
.
[Abstract] We use fluorescence recovery after photobleaching (FRAP) to calculate the diffusion coefficient of GFP in the nucleoplasm of fission yeast. The FRAP method can be generally used to measure the mobility of proteins inside the cell or its organelles.
In our experiment we only measured the diffusion of ...
Chromatin Fractionation Assay in Fission Yeast
Authors:
Tatsuki Kunoh
Tatsuki Kunoh
Affiliation:
Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan
For correspondence:
tkunoh06@gmail.com
Bio-protocol author page:
a1508
and
Toshiyuki Habu
Toshiyuki Habu
Affiliation:
Radiation Biology Center, Kyoto University, Kyoto, Japan
Bio-protocol author page:
a1509
,
date: 7/20/2014, 3144 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1185
.
[Abstract] The protein recruitment onto chromatin is a critical process for DNA metabolism, including DNA replication, DNA repair and DNA recombination. Especially DNA modification enzymes and checkpoint proteins are loaded onto DNA damage sites in a context-dependent manner. In our recent study (Kunoh and Habu, ...
Single-step Marker Switching in
Schizosaccharomyces pombe
Using a Lithium Acetate Transformation Protocol
Authors:
Simon David Brown
Simon David Brown
Affiliation:
Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK
Bio-protocol author page:
a3916
and
Alexander Lorenz
Alexander Lorenz
Affiliation:
Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK
For correspondence:
a.lorenz@abdn.ac.uk
Bio-protocol author page:
a3917
,
date: 12/20/2016, 1321 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.2075
.
[Abstract] The ability to utilize different selectable markers for tagging or mutating multiple genes in
Schizosaccharomyces pombe
is hampered by the historical use of only two selectable markers,
ura4
+
and
kanMX6
; the latter conferring resistance to the antibiotic G418 (geneticin). More markers have been described ...
1
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