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[
14
C] Linoleic Acid Uptake and Fractionation Assay in
Vibrio cholerae
Authors:
Sarah C. Plecha
Sarah C. Plecha
Affiliation:
Department of Immunology and Microbiology, Wayne State University School of Medicine, Michigan, USA
Bio-protocol author page:
a2783
and
Jeffrey H. Withey
Jeffrey H. Withey
Affiliation:
Department of Immunology and Microbiology, Wayne State University School of Medicine, Michigan, USA
For correspondence:
jwithey@wayne.edu
Bio-protocol author page:
a2784
,
date: 12/20/2015, 1242 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1682
.
[Abstract] The gram-negative curved bacillus
Vibrio cholerae
(
V. cholerae
) causes the severe diarrheal illness cholera. The work presented here is to assess whether unsaturated fatty acids (UFAs), such as linoleic acid, have the potential to directly affect proteins involved in DNA binding because they are able ...
A Filter Binding Assay to Quantify the Association of Cyclic di-GMP to Proteins
Authors:
Disha Srivastava
Disha Srivastava
Affiliation:
Department of Microbiology and Microbial Genetics, Michigan State University, East Lansing, USA
Bio-protocol author page:
a1981
and
Christopher M. Waters
Christopher M. Waters
Affiliation:
Department of Microbiology and Microbial Genetics, Michigan State University, East Lansing, USA
For correspondence:
watersc3@msu.edu
Bio-protocol author page:
a1982
,
date: 2/5/2015, 3020 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1394
.
[Abstract] Cyclic di-GMP (c-di-GMP) is a ubiquitous second messenger that regulates many processes in bacteria including biofilm formation, motility, and virulence (Hengge, 2009). Analysis of c-di-GMP binding properties of bacterial proteins is an important step to characterize c-di-GMP signaling pathways. C-di-GMP ...
Infant Rabbit Colonization Competition Assays
Authors:
Yang Fu
Yang Fu
Affiliation:
Department of Microbiology and Immunobiology, Harvard Medical School, Boston, USA
For correspondence:
yang_fu@hms.harvard.edu
Bio-protocol author page:
a1395
and
John J. Mekalanos
John J. Mekalanos
Affiliation:
Department of Microbiology and Immunobiology, Harvard Medical School, Boston, USA
For correspondence:
john_mekalanos@hms.harvard.edu
Bio-protocol author page:
a1396
,
date: 6/5/2014, 3058 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1147
.
[Abstract] Enteric pathogenic bacteria such as
Vibrio cholerae
and enteropathogenic
Escherichia coli
(
E. coli
) cause life-threatening diarrheal diseases that have afflicted humans for centuries. Understanding the effectors required for intestinal colonization is very important to research on bacteria pathogenesis, ...
Assay for Adherence of
Vibrio cholerae
to Eukaryotic Cell Lines
Authors:
Amit K. Dey
Amit K. Dey
Affiliation:
Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, Council of Scientific and Industrial Research, Kolkata, India
Bio-protocol author page:
a1296
,
Abha Bhagat
Abha Bhagat
Affiliation:
Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, Council of Scientific and Industrial Research, Kolkata, India
Bio-protocol author page:
a1297
and
Rukhsana Chowdhury
Rukhsana Chowdhury
Affiliation:
Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, Council of Scientific and Industrial Research, Kolkata, India
For correspondence:
rukhsana@iicb.res.in
Bio-protocol author page:
a1298
,
date: 4/20/2014, 3691 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1105
.
[Abstract]
Vibrio cholerae
(
V. cholerae
) colonizes the intestinal epithelium and secretes cholera toxin (CT), a potent enterotoxin that causes severe fluid loss characteristic of the disease cholera.
V. cholerae
is a non-invasive Gram-negative bacterium that adheres to intestinal cells as well as a variety of ...
Differential in vivo Thiol Trapping with N-ethylmaleimide (NEM) and 4-acetamido-4'-maleimidylstilbene-2,2'-disulfonic acid (AMS)
Author:
Wei-Yun (Winnie) Wholey
Wei-Yun (Winnie) Wholey
Affiliation:
Pediatrics and Communicable Diseases, University of Michigan, Medical School, Ann Arbor, MI, USA
For correspondence:
weiyun@umich.edu
Bio-protocol author page:
a126
,
date: 10/20/2012, 6879 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.272
.
[Abstract] This protocol is used to compare the
in vivo
redox status of
Escherichia coli
and
Vibrio cholerae
protein before and after HOCl treatment. For example, I examined whether the EF-Tu protein is reduced or oxidized in the referenced publication. This protocol should work for other proteins and other oxidative ...
Preparation of Genomic Overexpression Library
Author:
Wei-Yun (Winnie) Wholey
Wei-Yun (Winnie) Wholey
Affiliation:
Pediatrics and Communicable Diseases, University of Michigan, Medical School, Ann Arbor, MI, USA
For correspondence:
weiyun@umich.edu
Bio-protocol author page:
a126
,
date: 10/5/2012, 4619 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.271
.
[Abstract] This protocol is used to identify/select for
Escherichia coli
genes that, when overexpressed in
Vibrio cholerae Hsp33
(hslO gene) deletion mutant, protect against oxidative heat stress and, by extension, against HOCl-mediated protein damage. In the referenced publication, I found that
V. cholerae
mutant ...
1
Differential in vivo Thiol Trapping with N-ethylmaleimide (NEM) and 4-acetamido-4'-maleimidylstilbene-2,2'-disulfonic acid (AMS)
Author:
Wei-Yun (Winnie) Wholey
Wei-Yun (Winnie) Wholey
Affiliation:
Pediatrics and Communicable Diseases, University of Michigan, Medical School, Ann Arbor, MI, USA
For correspondence:
weiyun@umich.edu
Bio-protocol author page:
a126
,
date: 10/20/2012, 6879 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.272
.
[Abstract] This protocol is used to compare the
in vivo
redox status of
Escherichia coli
and
Vibrio cholerae
protein before and after HOCl treatment. For example, I examined whether the EF-Tu protein is reduced or oxidized in the referenced publication. This protocol should work for other proteins and other oxidative ...
Preparation of Genomic Overexpression Library
Author:
Wei-Yun (Winnie) Wholey
Wei-Yun (Winnie) Wholey
Affiliation:
Pediatrics and Communicable Diseases, University of Michigan, Medical School, Ann Arbor, MI, USA
For correspondence:
weiyun@umich.edu
Bio-protocol author page:
a126
,
date: 10/5/2012, 4619 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.271
.
[Abstract] This protocol is used to identify/select for
Escherichia coli
genes that, when overexpressed in
Vibrio cholerae Hsp33
(hslO gene) deletion mutant, protect against oxidative heat stress and, by extension, against HOCl-mediated protein damage. In the referenced publication, I found that
V. cholerae
mutant ...
Assay for Adherence of
Vibrio cholerae
to Eukaryotic Cell Lines
Authors:
Amit K. Dey
Amit K. Dey
Affiliation:
Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, Council of Scientific and Industrial Research, Kolkata, India
Bio-protocol author page:
a1296
,
Abha Bhagat
Abha Bhagat
Affiliation:
Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, Council of Scientific and Industrial Research, Kolkata, India
Bio-protocol author page:
a1297
and
Rukhsana Chowdhury
Rukhsana Chowdhury
Affiliation:
Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, Council of Scientific and Industrial Research, Kolkata, India
For correspondence:
rukhsana@iicb.res.in
Bio-protocol author page:
a1298
,
date: 4/20/2014, 3691 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1105
.
[Abstract]
Vibrio cholerae
(
V. cholerae
) colonizes the intestinal epithelium and secretes cholera toxin (CT), a potent enterotoxin that causes severe fluid loss characteristic of the disease cholera.
V. cholerae
is a non-invasive Gram-negative bacterium that adheres to intestinal cells as well as a variety of ...
Infant Rabbit Colonization Competition Assays
Authors:
Yang Fu
Yang Fu
Affiliation:
Department of Microbiology and Immunobiology, Harvard Medical School, Boston, USA
For correspondence:
yang_fu@hms.harvard.edu
Bio-protocol author page:
a1395
and
John J. Mekalanos
John J. Mekalanos
Affiliation:
Department of Microbiology and Immunobiology, Harvard Medical School, Boston, USA
For correspondence:
john_mekalanos@hms.harvard.edu
Bio-protocol author page:
a1396
,
date: 6/5/2014, 3058 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1147
.
[Abstract] Enteric pathogenic bacteria such as
Vibrio cholerae
and enteropathogenic
Escherichia coli
(
E. coli
) cause life-threatening diarrheal diseases that have afflicted humans for centuries. Understanding the effectors required for intestinal colonization is very important to research on bacteria pathogenesis, ...
A Filter Binding Assay to Quantify the Association of Cyclic di-GMP to Proteins
Authors:
Disha Srivastava
Disha Srivastava
Affiliation:
Department of Microbiology and Microbial Genetics, Michigan State University, East Lansing, USA
Bio-protocol author page:
a1981
and
Christopher M. Waters
Christopher M. Waters
Affiliation:
Department of Microbiology and Microbial Genetics, Michigan State University, East Lansing, USA
For correspondence:
watersc3@msu.edu
Bio-protocol author page:
a1982
,
date: 2/5/2015, 3020 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1394
.
[Abstract] Cyclic di-GMP (c-di-GMP) is a ubiquitous second messenger that regulates many processes in bacteria including biofilm formation, motility, and virulence (Hengge, 2009). Analysis of c-di-GMP binding properties of bacterial proteins is an important step to characterize c-di-GMP signaling pathways. C-di-GMP ...
[
14
C] Linoleic Acid Uptake and Fractionation Assay in
Vibrio cholerae
Authors:
Sarah C. Plecha
Sarah C. Plecha
Affiliation:
Department of Immunology and Microbiology, Wayne State University School of Medicine, Michigan, USA
Bio-protocol author page:
a2783
and
Jeffrey H. Withey
Jeffrey H. Withey
Affiliation:
Department of Immunology and Microbiology, Wayne State University School of Medicine, Michigan, USA
For correspondence:
jwithey@wayne.edu
Bio-protocol author page:
a2784
,
date: 12/20/2015, 1242 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1682
.
[Abstract] The gram-negative curved bacillus
Vibrio cholerae
(
V. cholerae
) causes the severe diarrheal illness cholera. The work presented here is to assess whether unsaturated fatty acids (UFAs), such as linoleic acid, have the potential to directly affect proteins involved in DNA binding because they are able ...
1
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