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Rust Removal Experiments

Authors: Mahmoud Kamal Ahmadi
Mahmoud Kamal AhmadiAffiliation: Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, USA
Bio-protocol author page: a3031
 and Blaine A. Pfeifer
Blaine A. PfeiferAffiliation: Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, USA
For correspondence: blainepf@buffalo.edu
Bio-protocol author page: a3032
date: 4/5/2016, 2081 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1776.

[Abstract] Iron oxidation, known as rust formation, causes enormous loss in term of property damages and associated economic risks. Depending on the degree of formation, rust consists of several layers of iron in different oxidation states. The brownish top layer is mostly iron (III) oxide-hydroxide [FeO(OH), ...

Construction and Screening of a Transposon Insertion Library of Yersinia enterocolitica (YeO3-R1)

Authors: Maria Pajunen
Maria PajunenAffiliation: Department of Biosciences, Division of Biochemistry and Biotechnology, University of Helsinki, Helsinki, Finland
Bio-protocol author page: a79
Elise Pinta
Elise PintaAffiliation: Department of Medical Biochemistry and Genetics, University of Turku, Turku, Finland
Bio-protocol author page: a80
 and Mikael Skurnik
Mikael SkurnikAffiliation: Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland
For correspondence: mikael.skurnik@helsinki.fi
Bio-protocol author page: a78
date: 8/5/2012, 6596 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.246.

[Abstract] The Mu-transposon system is one of the best characterized transposition systems. Under minimal in vitro set-up, Mu transposition requires only a simple reaction buffer, MuA transposase protein, mini-Mu transposon DNA (donor) and target DNA. The reaction proceeds via initial assembly of the transposition ...
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Construction and Screening of a Transposon Insertion Library of Yersinia enterocolitica (YeO3-R1)

Authors: Maria Pajunen
Maria PajunenAffiliation: Department of Biosciences, Division of Biochemistry and Biotechnology, University of Helsinki, Helsinki, Finland
Bio-protocol author page: a79
Elise Pinta
Elise PintaAffiliation: Department of Medical Biochemistry and Genetics, University of Turku, Turku, Finland
Bio-protocol author page: a80
 and Mikael Skurnik
Mikael SkurnikAffiliation: Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland
For correspondence: mikael.skurnik@helsinki.fi
Bio-protocol author page: a78
date: 8/5/2012, 6596 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.246.

[Abstract] The Mu-transposon system is one of the best characterized transposition systems. Under minimal in vitro set-up, Mu transposition requires only a simple reaction buffer, MuA transposase protein, mini-Mu transposon DNA (donor) and target DNA. The reaction proceeds via initial assembly of the transposition ...

Rust Removal Experiments

Authors: Mahmoud Kamal Ahmadi
Mahmoud Kamal AhmadiAffiliation: Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, USA
Bio-protocol author page: a3031
 and Blaine A. Pfeifer
Blaine A. PfeiferAffiliation: Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, USA
For correspondence: blainepf@buffalo.edu
Bio-protocol author page: a3032
date: 4/5/2016, 2081 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1776.

[Abstract] Iron oxidation, known as rust formation, causes enormous loss in term of property damages and associated economic risks. Depending on the degree of formation, rust consists of several layers of iron in different oxidation states. The brownish top layer is mostly iron (III) oxide-hydroxide [FeO(OH), ...
1 
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