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Multiplex Gene Editing via CRISPR/Cas9 System in Sheep
Authors:
Yiyuan Niu
Yiyuan Niu
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, China
Bio-protocol author page:
a4803
,
Yi Ding
Yi Ding
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, China
Bio-protocol author page:
a4804
,
Xiaolong Wang
Xiaolong Wang
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, China
Bio-protocol author page:
a4805
and
Yulin Chen
Yulin Chen
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, China
For correspondence:
chenyulin@nwafu.edu.cn
Bio-protocol author page:
a4802
,
date: 7/5/2017, 137 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.2385
.
[Abstract] Sheep is a major large animal model for studying development and disease in biomedical research. We utilized CRISPR/Cas9 system successfully to modify multiple genes in sheep. Here we provide a detailed protocol for one-cell-stage embryo manipulation by co-injecting Cas9 mRNA and RNA guides targeting ...
1
Multiplex Gene Editing via CRISPR/Cas9 System in Sheep
Authors:
Yiyuan Niu
Yiyuan Niu
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, China
Bio-protocol author page:
a4803
,
Yi Ding
Yi Ding
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, China
Bio-protocol author page:
a4804
,
Xiaolong Wang
Xiaolong Wang
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, China
Bio-protocol author page:
a4805
and
Yulin Chen
Yulin Chen
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, China
For correspondence:
chenyulin@nwafu.edu.cn
Bio-protocol author page:
a4802
,
date: 7/5/2017, 137 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.2385
.
[Abstract] Sheep is a major large animal model for studying development and disease in biomedical research. We utilized CRISPR/Cas9 system successfully to modify multiple genes in sheep. Here we provide a detailed protocol for one-cell-stage embryo manipulation by co-injecting Cas9 mRNA and RNA guides targeting ...
1
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