Mammalia

Generation of Mutant Pigs by Direct Pronuclear Microinjection of CRISPR/Cas9 Plasmid Vectors

Authors: Chin-kai Chuang
Chin-kai ChuangAffiliation: Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Hsinchu City, Taiwan
For correspondence: jkjuang@mail.atri.org.tw
Bio-protocol author page: a4611
, Ching-Fu Tu
Ching-Fu TuAffiliation: Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Hsinchu City, Taiwan
Bio-protocol author page: a4612
and Chien-Hong Chen
Chien-Hong ChenAffiliation: Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Taichung, Taiwan
Present address: Lee Women’s Hospital, Reproductive Medicine Center, Taichung City, Taiwan
Bio-protocol author page: a4613
, date: 6/5/2017, 326 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2321.

[Abstract] A set of Cas9 and single guide CRISPR RNA expression vectors was constructed. Only a very simple procedure was needed to prepare specific single-guide RNA expression vectors with high target accuracy. Since the de novo zygotic transcription had been detected in mouse embryo at the 1-cell stage, the ...

Generation of Mutant Pigs by Direct Pronuclear Microinjection of CRISPR/Cas9 Plasmid Vectors

Authors: Chin-kai Chuang
Chin-kai ChuangAffiliation: Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Hsinchu City, Taiwan
For correspondence: jkjuang@mail.atri.org.tw
Bio-protocol author page: a4611
, Ching-Fu Tu
Ching-Fu TuAffiliation: Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Hsinchu City, Taiwan
Bio-protocol author page: a4612
and Chien-Hong Chen
Chien-Hong ChenAffiliation: Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Taichung, Taiwan
Present address: Lee Women’s Hospital, Reproductive Medicine Center, Taichung City, Taiwan
Bio-protocol author page: a4613
, date: 6/5/2017, 326 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2321.

Mammalia

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