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Xylem Sap Extraction Method from Hop Plants

Authors: Marko Flajšman*
Marko FlajšmanAffiliation: Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
Bio-protocol author page: a4167
Stanislav Mandelc*
Stanislav MandelcAffiliation: Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
Bio-protocol author page: a4168
Sebastjan Radišek*
Sebastjan RadišekAffiliation: Slovenian Institute of Hop Research and Brewing, Zalec, Slovenia
Bio-protocol author page: a4169
 and Branka Javornik
Branka JavornikAffiliation: Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
For correspondence: branka.javornik@bf.uni-lj.si
Bio-protocol author page: a4170
 (*contributed equally to this work) date: 3/20/2017, 771 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2172.

[Abstract] Verticillium wilt is one of the most important diseases on hop that significantly influence continuation of production on affected areas. It is caused by the soil borne vascular pathogen Verticillium nonalfalfae, which infects plants through the roots and then advances through the vascular (xylem) system. ...

Pathogenicity Assay of Verticillium nonalfalfae on Hop Plants

Authors: Marko Flajšman*
Marko FlajšmanAffiliation: Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
Bio-protocol author page: a4167
Sebastjan Radišek*
Sebastjan RadišekAffiliation: Slovenian Institute of Hop Research and Brewing, Zalec, Slovenia
Bio-protocol author page: a4169
 and Branka Javornik
Branka JavornikAffiliation: Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
For correspondence: branka.javornik@bf.uni-lj.si
Bio-protocol author page: a4170
 (*contributed equally to this work) date: 3/20/2017, 689 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2171.

[Abstract] Verticillium nonalfalfae is a soil-borne plant pathogen that infects its hosts through roots. It spreads in the plant’s xylem and causes wilt disease symptoms by secreting different virulence factors. Hop (Humulus lupulus) is a primary host of V. nonalfalfae, so it is used as a model plant for studying ...

Penetration Assays, Fungal Recovery and Pathogenicity Assays for Verticillium dahliae

Authors: Yun-Long Zhao
Yun-Long ZhaoAffiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
For correspondence: zyl714@126.com
Bio-protocol author page: a4092
Tao Zhang
Tao Zhang Affiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
Bio-protocol author page: a4093
 and Hui-Shan Guo
Hui-Shan GuoAffiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
Bio-protocol author page: a2721
date: 2/20/2017, 880 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2133.

[Abstract] Verticillium dahliae is a soil-borne phytopathogenic fungus that infects host roots and proliferates in vascular tissues. The great loss of economically important crop caused by V. dahliae has raised worldwide concern, however, little is known about the mechanism of its pathogenicity (Klosterman et ...
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Penetration Assays, Fungal Recovery and Pathogenicity Assays for Verticillium dahliae

Authors: Yun-Long Zhao
Yun-Long ZhaoAffiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
For correspondence: zyl714@126.com
Bio-protocol author page: a4092
Tao Zhang
Tao Zhang Affiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
Bio-protocol author page: a4093
 and Hui-Shan Guo
Hui-Shan GuoAffiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
Bio-protocol author page: a2721
date: 2/20/2017, 880 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2133.

[Abstract] Verticillium dahliae is a soil-borne phytopathogenic fungus that infects host roots and proliferates in vascular tissues. The great loss of economically important crop caused by V. dahliae has raised worldwide concern, however, little is known about the mechanism of its pathogenicity (Klosterman et ...

Xylem Sap Extraction Method from Hop Plants

Authors: Marko Flajšman*
Marko FlajšmanAffiliation: Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
Bio-protocol author page: a4167
Stanislav Mandelc*
Stanislav MandelcAffiliation: Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
Bio-protocol author page: a4168
Sebastjan Radišek*
Sebastjan RadišekAffiliation: Slovenian Institute of Hop Research and Brewing, Zalec, Slovenia
Bio-protocol author page: a4169
 and Branka Javornik
Branka JavornikAffiliation: Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
For correspondence: branka.javornik@bf.uni-lj.si
Bio-protocol author page: a4170
 (*contributed equally to this work) date: 3/20/2017, 771 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2172.

[Abstract] Verticillium wilt is one of the most important diseases on hop that significantly influence continuation of production on affected areas. It is caused by the soil borne vascular pathogen Verticillium nonalfalfae, which infects plants through the roots and then advances through the vascular (xylem) system. ...

Pathogenicity Assay of Verticillium nonalfalfae on Hop Plants

Authors: Marko Flajšman*
Marko FlajšmanAffiliation: Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
Bio-protocol author page: a4167
Sebastjan Radišek*
Sebastjan RadišekAffiliation: Slovenian Institute of Hop Research and Brewing, Zalec, Slovenia
Bio-protocol author page: a4169
 and Branka Javornik
Branka JavornikAffiliation: Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
For correspondence: branka.javornik@bf.uni-lj.si
Bio-protocol author page: a4170
 (*contributed equally to this work) date: 3/20/2017, 689 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2171.

[Abstract] Verticillium nonalfalfae is a soil-borne plant pathogen that infects its hosts through roots. It spreads in the plant’s xylem and causes wilt disease symptoms by secreting different virulence factors. Hop (Humulus lupulus) is a primary host of V. nonalfalfae, so it is used as a model plant for studying ...
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