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Plant Tissue Trypan Blue Staining During Phytopathogen Infection

Authors: Nuria Fernández-Bautista
Nuria Fernández-BautistaAffiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) – Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Parque Científico y Tecnológico, UPM, Campus de Montegancedo, Ctra M-40, km 38, 28223, Pozuelo de Alarcón (Madrid), Spain
Bio-protocol author page: a3925
, José Alfonso Domínguez-Núñez
José Alfonso Domínguez-NúñezAffiliation: Departamento de Sistemas y Recursos Naturales, Escuela Técnica Superior de Ingeniería de Montes, Forestal y del Medio Natural, Ciudad Universitaria s/n, 28040, Madrid, Spain
Bio-protocol author page: a3926
, M. Mar Castellano Moreno
M. Mar Castellano MorenoAffiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) – Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Parque Científico y Tecnológico, UPM, Campus de Montegancedo, Ctra M-40, km 38, 28223, Pozuelo de Alarcón (Madrid), Spain
Bio-protocol author page: a3927
 and Marta Berrocal-Lobo
Marta Berrocal-LoboAffiliation 1: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) – Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Parque Científico y Tecnológico, UPM, Campus de Montegancedo, Ctra M-40, km 38, 28223, Pozuelo de Alarcón (Madrid), Spain
Affiliation 2: Departamento de Sistemas y Recursos Naturales, Escuela Técnica Superior de Ingeniería de Montes, Forestal y del Medio Natural, Ciudad Universitaria s/n, 28040, Madrid, Spain
For correspondence: m.berrocal@upm.es
Bio-protocol author page: a3928
,  date: 12/20/2016, 2078 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2078.

[Abstract] In this protocol plant tissue is stained with trypan blue dye allowing the researcher to visualize cell death. Specifically this method avoids the use of the carcinogen compound chloral hydrate, making this classical method of staining safer and faster than never. The protocol is applied specifically ...

An Assay to Study Botrytis cinerea-infected Grapevine Leaves Primed with Pseudomonas fluorescens

Authors: Charlotte Gruau
Charlotte GruauAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3539
, Patricia Trotel-Aziz
Patricia Trotel-AzizAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3540
, Bas Verhagen
Bas VerhagenAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3541
, Sandra Villaume
Sandra VillaumeAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3542
, Fanja Rabenoelina
Fanja RabenoelinaAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3543
, Barbara Courteaux
Barbara CourteauxAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3544
, Christophe Clément
Christophe ClémentAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3545
, Fabienne Baillieul
Fabienne BaillieulAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3546
 and Aziz Aziz
Aziz AzizAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
For correspondence: aziz.aziz@univ-reims.fr
Bio-protocol author page: a3547
,  date: 10/5/2016, 1588 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1943.

[Abstract] Grapevine (Vitis vinifera L.) is susceptible to an array of diseases among them the grey mold caused by the necrotrophic fungus Botrytis cinerea that decreases grape productivity and quality. To ensure a satisfactory yield and harvest quality numerous chemical fungicides are required, but they have ...
1 

An Assay to Study Botrytis cinerea-infected Grapevine Leaves Primed with Pseudomonas fluorescens

Authors: Charlotte Gruau
Charlotte GruauAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3539
, Patricia Trotel-Aziz
Patricia Trotel-AzizAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3540
, Bas Verhagen
Bas VerhagenAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3541
, Sandra Villaume
Sandra VillaumeAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3542
, Fanja Rabenoelina
Fanja RabenoelinaAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3543
, Barbara Courteaux
Barbara CourteauxAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3544
, Christophe Clément
Christophe ClémentAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3545
, Fabienne Baillieul
Fabienne BaillieulAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
Bio-protocol author page: a3546
 and Aziz Aziz
Aziz AzizAffiliation: URVVC EA4707, UFR Sciences, University of Reims, Reims, France
For correspondence: aziz.aziz@univ-reims.fr
Bio-protocol author page: a3547
,  date: 10/5/2016, 1588 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1943.

[Abstract] Grapevine (Vitis vinifera L.) is susceptible to an array of diseases among them the grey mold caused by the necrotrophic fungus Botrytis cinerea that decreases grape productivity and quality. To ensure a satisfactory yield and harvest quality numerous chemical fungicides are required, but they have ...
1 
Fungi
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Expand <font color='#517792'>Alternaria</font>Alternaria
Expand <font color='#517792'>Aspergillus</font>Aspergillus
Expand <font color='#517792'>Blastomyces</font>Blastomyces
Collapse <font color='#e17009'>Botrytis</font>Botrytis
Expand <font color='#517792'>Candida</font>Candida
Expand <font color='#517792'>Colletotrichum</font>Colletotrichum
Expand <font color='#517792'>Drechmeria</font>Drechmeria
Expand <font color='#517792'>Fusarium</font>Fusarium
Expand <font color='#517792'>Lobaria</font>Lobaria
Expand <font color='#517792'>Magnaporthe </font>Magnaporthe
Expand <font color='#517792'>Neurospora</font>Neurospora
Expand <font color='#517792'>Ogataea</font>Ogataea
Expand <font color='#517792'>Paracoccidioides</font>Paracoccidioides
Expand <font color='#517792'>Penicillium</font>Penicillium
Expand <font color='#517792'>Phytophthora</font>Phytophthora
Expand <font color='#517792'>Pichia</font>Pichia
Expand <font color='#517792'>Piriformospora </font>Piriformospora
Expand <font color='#517792'>Saccharomyces </font>Saccharomyces
Expand <font color='#517792'>Schizosaccharomyces </font>Schizosaccharomyces
Expand <font color='#517792'>Sclerotinia</font>Sclerotinia
Expand <font color='#517792'>Trichoderma</font>Trichoderma
Expand <font color='#517792'>Ustilago</font>Ustilago
Expand <font color='#517792'>Verticillium</font>Verticillium
Expand <font color='#517792'>Xanthoparmelia</font>Xanthoparmelia
Expand <font color='#517792'>Xanthoria</font>Xanthoria


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