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Chitin Extraction and Content Measurement in Magnaporthe oryzae

Authors: Xinyu Liu
Xinyu LiuAffiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China
Bio-protocol author page: a4123
 and Zhengguang Zhang
Zhengguang ZhangAffiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China
For correspondence: zhgzhang@njau.edu.cn
Bio-protocol author page: a4208
,  date: 3/5/2017, 789 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2164.

[Abstract] Chitin is a linear polysaccharide composed of β (1→4)-linked N-acetylglucosamine (GlcNAc) residues. In fungi, chitin is an important component of the cell wall. Here, we provide a protocol to measure the chitin content of fungal cells using Magnaporthe oryzae as an example. ...

Chromatin Immunoprecipitation (ChIP) Assay for Detecting Direct and Indirect Protein – DNA Interactions in Magnaporthe oryzae

Authors: Gang Li
Gang LiAffiliation: Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, USA
Bio-protocol author page: a2693
, Margarita Marroquin-Guzman
Margarita Marroquin-GuzmanAffiliation: Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, USA
Bio-protocol author page: a2694
 and Richard A. Wilson
Richard A. WilsonAffiliation: Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, USA
For correspondence: rwilson10@unl.edu
Bio-protocol author page: a2695
,  date: 11/5/2015, 2962 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1643.

[Abstract] Chromatin immunoprecipitation (ChIP) is a powerful technology for analyzing protein-DNA interactions in cells. Robust ChIP procedures have been established for investigating direct interactions between protein and DNA. However, detecting indirect protein-DNA interactions in vivo is challenging. Recently, ...
1 

Chromatin Immunoprecipitation (ChIP) Assay for Detecting Direct and Indirect Protein – DNA Interactions in Magnaporthe oryzae

Authors: Gang Li
Gang LiAffiliation: Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, USA
Bio-protocol author page: a2693
, Margarita Marroquin-Guzman
Margarita Marroquin-GuzmanAffiliation: Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, USA
Bio-protocol author page: a2694
 and Richard A. Wilson
Richard A. WilsonAffiliation: Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, USA
For correspondence: rwilson10@unl.edu
Bio-protocol author page: a2695
,  date: 11/5/2015, 2962 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1643.

[Abstract] Chromatin immunoprecipitation (ChIP) is a powerful technology for analyzing protein-DNA interactions in cells. Robust ChIP procedures have been established for investigating direct interactions between protein and DNA. However, detecting indirect protein-DNA interactions in vivo is challenging. Recently, ...

Chitin Extraction and Content Measurement in Magnaporthe oryzae

Authors: Xinyu Liu
Xinyu LiuAffiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China
Bio-protocol author page: a4123
 and Zhengguang Zhang
Zhengguang ZhangAffiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China
For correspondence: zhgzhang@njau.edu.cn
Bio-protocol author page: a4208
,  date: 3/5/2017, 789 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2164.

[Abstract] Chitin is a linear polysaccharide composed of β (1→4)-linked N-acetylglucosamine (GlcNAc) residues. In fungi, chitin is an important component of the cell wall. Here, we provide a protocol to measure the chitin content of fungal cells using Magnaporthe oryzae as an example. ...
1 
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