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MNase Digestion for Nucleosome Mapping in Neurospora

Authors: Cigdem Sancar
Cigdem SancarAffiliation: Center for Circadian Biology, California Center for Algal Biotechnology, Division of Biological Sciences, University of California, San Diego, USA
Bio-protocol author page: a3166
, Gencer Sancar
Gencer SancarAffiliation: Gene Expression Laboratory, Salk Institute, San Diego, USA
Bio-protocol author page: a3167
 and Michael Brunner
Michael BrunnerAffiliation: Biochemistry Center, University of Heidelberg, Heidelberg, Germany
For correspondence: michael.brunner@bzh.uni-heidelberg.de
Bio-protocol author page: a3168
,  date: 6/5/2016, 1463 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1825.

[Abstract] Digestion of chromatin by micrococcal nuclease MNase followed by high throughput sequencing allows us to determine the location and occupancy of nucleosomes on the genome. Here in this protocol we have described optimized conditions of MNase digestion of filamentous fungus Neurospora crassa chromatin ...

Density Gradient Centrifugation for Enrichment and Identification of GFP-tagged Chitosomal Microvesicles of Filamentous Fungi

Authors: Jorge Verdín*
Jorge VerdínAffiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Mexico
Present address: Industrial Biotechnology, Jalisco State Research and Technology Assistance Center (CIATEJ), Guadalajara, Mexico
Bio-protocol author page: a2615
, Eddy Sánchez-León*
Eddy Sánchez-LeónAffiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Mexico
Bio-protocol author page: a2616
, Rosa Fajardo-Somera
Rosa Fajardo-SomeraAffiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Mexico
Bio-protocol author page: a2617
, Carlos A. Leal Morales
Carlos A. Leal MoralesAffiliation: Department of Biology, Campus Guanajuato, University of Guanajuato, Guanajuato, Mexico
Bio-protocol author page: a2618
, Salomón Bartnicki-García
Salomón Bartnicki-GarcíaAffiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Mexico
Bio-protocol author page: a2619
 and Meritxell Riquelme
Meritxell RiquelmeAffiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Mexico
For correspondence: riquelme@cicese.mx
Bio-protocol author page: a2620
 (*contributed equally to this work),  date: 10/5/2015, 3427 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1611.

[Abstract] Density gradient centrifugation has been utilized to characterize the subcellular distribution of physiologically relevant enzymes in yeasts and filamentous fungi (Leal-Morales et al., 1988; Martínez et al., 1989; Kamada et al., 1991). This approach is now potentiated by protein tagging and live imaging ...
1 

Density Gradient Centrifugation for Enrichment and Identification of GFP-tagged Chitosomal Microvesicles of Filamentous Fungi

Authors: Jorge Verdín*
Jorge VerdínAffiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Mexico
Present address: Industrial Biotechnology, Jalisco State Research and Technology Assistance Center (CIATEJ), Guadalajara, Mexico
Bio-protocol author page: a2615
, Eddy Sánchez-León*
Eddy Sánchez-LeónAffiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Mexico
Bio-protocol author page: a2616
, Rosa Fajardo-Somera
Rosa Fajardo-SomeraAffiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Mexico
Bio-protocol author page: a2617
, Carlos A. Leal Morales
Carlos A. Leal MoralesAffiliation: Department of Biology, Campus Guanajuato, University of Guanajuato, Guanajuato, Mexico
Bio-protocol author page: a2618
, Salomón Bartnicki-García
Salomón Bartnicki-GarcíaAffiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Mexico
Bio-protocol author page: a2619
 and Meritxell Riquelme
Meritxell RiquelmeAffiliation: Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, Mexico
For correspondence: riquelme@cicese.mx
Bio-protocol author page: a2620
 (*contributed equally to this work),  date: 10/5/2015, 3427 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1611.

[Abstract] Density gradient centrifugation has been utilized to characterize the subcellular distribution of physiologically relevant enzymes in yeasts and filamentous fungi (Leal-Morales et al., 1988; Martínez et al., 1989; Kamada et al., 1991). This approach is now potentiated by protein tagging and live imaging ...

MNase Digestion for Nucleosome Mapping in Neurospora

Authors: Cigdem Sancar
Cigdem SancarAffiliation: Center for Circadian Biology, California Center for Algal Biotechnology, Division of Biological Sciences, University of California, San Diego, USA
Bio-protocol author page: a3166
, Gencer Sancar
Gencer SancarAffiliation: Gene Expression Laboratory, Salk Institute, San Diego, USA
Bio-protocol author page: a3167
 and Michael Brunner
Michael BrunnerAffiliation: Biochemistry Center, University of Heidelberg, Heidelberg, Germany
For correspondence: michael.brunner@bzh.uni-heidelberg.de
Bio-protocol author page: a3168
,  date: 6/5/2016, 1463 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1825.

[Abstract] Digestion of chromatin by micrococcal nuclease MNase followed by high throughput sequencing allows us to determine the location and occupancy of nucleosomes on the genome. Here in this protocol we have described optimized conditions of MNase digestion of filamentous fungus Neurospora crassa chromatin ...
1 
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