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Extraction, Purification and Quantification of Diffusible Signal Factor Family Quorum-sensing Signal Molecules in Xanthomonas oryzae pv. oryzae

Authors: Lian Zhou
Lian ZhouAffiliation 1: State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
Affiliation 2: Zhiyuan Innovative Research Center, Shanghai Jiao Tong University, Shanghai, China
Bio-protocol author page: a4225
, Xing-Yu Wang
Xing-Yu WangAffiliation: State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
Bio-protocol author page: a4226
, Wei Zhang
Wei ZhangAffiliation: State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
Bio-protocol author page: a4227
, Shuang Sun
Shuang SunAffiliation: State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
Bio-protocol author page: a4228
 and Ya-Wen He
Ya-Wen HeAffiliation: State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
For correspondence: yawenhe@sjtu.edu.cn
Bio-protocol author page: a4229
,  date: 3/20/2017, 672 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2190.

[Abstract] Bacteria use quorum-sensing (QS) systems to monitor and regulate their population density. Bacterial QS involves small molecules that act as signals for bacterial communication. Many Gram-negative bacterial pathogens use a class of widely conserved molecules, called diffusible signal factor (DSF) family ...

Isolation of Outer Membrane Vesicles from Phytopathogenic Xanthomonas campestris pv. campestris

Authors: Gideon Mordukhovich
Gideon MordukhovichAffiliation 1: Department of Plant Pathology and Weed Research, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Affiliation 2: Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot, Israel
For correspondence: gideon.mordukhovic@mail.huji.ac.il
Bio-protocol author page: a4144
 and Ofir Bahar
Ofir BaharAffiliation: Department of Plant Pathology and Weed Research, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
For correspondence: ofirb@agri.gov.il
Bio-protocol author page: a4145
,  date: 3/5/2017, 648 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2160.

[Abstract] Gram-negative bacteria naturally release outer membrane vesicles (OMVs) to the surrounding environment. OMVs contribute to multiple processes, such as cell-cell communication, delivery of enzymes and toxins, resistance to environmental stresses and pathogenesis. Little is known about OMVs produced by ...

Bioassay of Xanthomonas albilineans Attachment on Sugarcane Leaves

Authors: Imène Mensi
Imène MensiAffiliation: Department of Biological Sciences and Plant Protection, Higher Agronomic Institute of Chott-Mariem, Sousse, Tunisia
Bio-protocol author page: a3989
, Jean-Heinrich Daugrois
Jean-Heinrich DaugroisAffiliation: UMR BGPI, CIRAD, Montpellier, France
Bio-protocol author page: a3990
 and Philippe Rott
Philippe RottAffiliation: Department of Plant Pathology, University of Florida, Everglades Research and Education Center, Belle Glade, USA
For correspondence: pcrott@ufl.edu
Bio-protocol author page: a3991
,  date: 1/20/2017, 1008 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2111.

[Abstract] Sugarcane (interspecific hybrids of Saccharum species) is an economically important crop that provides 70% of raw table sugar production worldwide and contributes, in some countries, to bioethanol and electricity production. Leaf scald, caused by the bacterial plant pathogen Xanthomonas albilineans, ...

Quantification of Ethylene Production in Tomato Leaves Infected by Xanthomonas euvesicatoria

Authors: Jung-Gun Kim
Jung-Gun KimAffiliation: Department of Biology, Stanford University, Stanford, USA
Bio-protocol author page: a2886
, William Stork
William StorkAffiliation: Department of Biology, Stanford University, Stanford, USA
Bio-protocol author page: a2887
 and Mary Beth Mudgett
Mary Beth MudgettAffiliation: Department of Biology, Stanford University, Stanford, USA
For correspondence: mudgett@stanford.edu
Bio-protocol author page: a2888
,  date: 2/5/2016, 1948 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1723.

[Abstract] Ethylene is a gaseous plant hormone controlling fruit ripening, flower opening, leaf senescence as well as abscission, and disease symptom development. Ethylene plays a critical role in the bacterial pathogen Xanthomonas euvesicatoria (X. euvesicatoria)-elicited symptom development in tomato. This protocol ...

Assays to Assess Virulence of Xanthomonas axonopodis pv. manihotis on Cassava

Authors: Megan Cohn*
Megan CohnAffiliation: University of California, Berkeley, USA
Bio-protocol author page: a2351
, Mikel Shybut*
Mikel ShybutAffiliation: University of California, Berkeley, USA
Bio-protocol author page: a2352
, Douglas Dahlbeck
Douglas DahlbeckAffiliation: University of California, Berkeley, USA
Bio-protocol author page: a2353
 and Brian Staskawicz
Brian StaskawiczAffiliation: University of California, Berkeley, USA
For correspondence: stask@berkeley.edu
Bio-protocol author page: a2354
 (*contributed equally to this work),  date: 7/5/2015, 2072 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1522.

[Abstract] Cassava (Manihot esculenta) is a root crop that provides calories for people living in more than 100 tropical and subtropical countries and serves as a raw material for processing into starch and biofuels as well as feed for livestock (Howeler et al., 2013). Xanthomonas axonopodis pv. manihotis (Xam), ...

Drug Sensitivity Assay of Xanthomonas citri subsp. citri Using REMA Plate Method

Authors: Isabel C. Silva
Isabel C. SilvaAffiliation: Ciências Biológicas, Universidade Estadual Paulista (UNESP), Araraquara, Brazil
For correspondence: isabelcrs10@gmail.com
Bio-protocol author page: a468
 and Henrique Ferreira
Henrique FerreiraAffiliation: Ciências Biológicas, Universidade Estadual Paulista (UNESP), Araraquara, Brazil
Bio-protocol author page: a467
,  date: 8/20/2013, 3455 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.861.

[Abstract] Resazurin Microtiter Assay (REMA) is a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. Resazurin detects cell viability by converting from a nonfluorescent dye to the highly red fluorescent dye resorufin in response to chemical reduction of growth medium resulting ...
1 

Drug Sensitivity Assay of Xanthomonas citri subsp. citri Using REMA Plate Method

Authors: Isabel C. Silva
Isabel C. SilvaAffiliation: Ciências Biológicas, Universidade Estadual Paulista (UNESP), Araraquara, Brazil
For correspondence: isabelcrs10@gmail.com
Bio-protocol author page: a468
 and Henrique Ferreira
Henrique FerreiraAffiliation: Ciências Biológicas, Universidade Estadual Paulista (UNESP), Araraquara, Brazil
Bio-protocol author page: a467
,  date: 8/20/2013, 3455 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.861.

[Abstract] Resazurin Microtiter Assay (REMA) is a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. Resazurin detects cell viability by converting from a nonfluorescent dye to the highly red fluorescent dye resorufin in response to chemical reduction of growth medium resulting ...

Assays to Assess Virulence of Xanthomonas axonopodis pv. manihotis on Cassava

Authors: Megan Cohn*
Megan CohnAffiliation: University of California, Berkeley, USA
Bio-protocol author page: a2351
, Mikel Shybut*
Mikel ShybutAffiliation: University of California, Berkeley, USA
Bio-protocol author page: a2352
, Douglas Dahlbeck
Douglas DahlbeckAffiliation: University of California, Berkeley, USA
Bio-protocol author page: a2353
 and Brian Staskawicz
Brian StaskawiczAffiliation: University of California, Berkeley, USA
For correspondence: stask@berkeley.edu
Bio-protocol author page: a2354
 (*contributed equally to this work),  date: 7/5/2015, 2072 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1522.

[Abstract] Cassava (Manihot esculenta) is a root crop that provides calories for people living in more than 100 tropical and subtropical countries and serves as a raw material for processing into starch and biofuels as well as feed for livestock (Howeler et al., 2013). Xanthomonas axonopodis pv. manihotis (Xam), ...

Quantification of Ethylene Production in Tomato Leaves Infected by Xanthomonas euvesicatoria

Authors: Jung-Gun Kim
Jung-Gun KimAffiliation: Department of Biology, Stanford University, Stanford, USA
Bio-protocol author page: a2886
, William Stork
William StorkAffiliation: Department of Biology, Stanford University, Stanford, USA
Bio-protocol author page: a2887
 and Mary Beth Mudgett
Mary Beth MudgettAffiliation: Department of Biology, Stanford University, Stanford, USA
For correspondence: mudgett@stanford.edu
Bio-protocol author page: a2888
,  date: 2/5/2016, 1948 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1723.

[Abstract] Ethylene is a gaseous plant hormone controlling fruit ripening, flower opening, leaf senescence as well as abscission, and disease symptom development. Ethylene plays a critical role in the bacterial pathogen Xanthomonas euvesicatoria (X. euvesicatoria)-elicited symptom development in tomato. This protocol ...

Bioassay of Xanthomonas albilineans Attachment on Sugarcane Leaves

Authors: Imène Mensi
Imène MensiAffiliation: Department of Biological Sciences and Plant Protection, Higher Agronomic Institute of Chott-Mariem, Sousse, Tunisia
Bio-protocol author page: a3989
, Jean-Heinrich Daugrois
Jean-Heinrich DaugroisAffiliation: UMR BGPI, CIRAD, Montpellier, France
Bio-protocol author page: a3990
 and Philippe Rott
Philippe RottAffiliation: Department of Plant Pathology, University of Florida, Everglades Research and Education Center, Belle Glade, USA
For correspondence: pcrott@ufl.edu
Bio-protocol author page: a3991
,  date: 1/20/2017, 1008 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2111.

[Abstract] Sugarcane (interspecific hybrids of Saccharum species) is an economically important crop that provides 70% of raw table sugar production worldwide and contributes, in some countries, to bioethanol and electricity production. Leaf scald, caused by the bacterial plant pathogen Xanthomonas albilineans, ...

Extraction, Purification and Quantification of Diffusible Signal Factor Family Quorum-sensing Signal Molecules in Xanthomonas oryzae pv. oryzae

Authors: Lian Zhou
Lian ZhouAffiliation 1: State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
Affiliation 2: Zhiyuan Innovative Research Center, Shanghai Jiao Tong University, Shanghai, China
Bio-protocol author page: a4225
, Xing-Yu Wang
Xing-Yu WangAffiliation: State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
Bio-protocol author page: a4226
, Wei Zhang
Wei ZhangAffiliation: State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
Bio-protocol author page: a4227
, Shuang Sun
Shuang SunAffiliation: State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
Bio-protocol author page: a4228
 and Ya-Wen He
Ya-Wen HeAffiliation: State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
For correspondence: yawenhe@sjtu.edu.cn
Bio-protocol author page: a4229
,  date: 3/20/2017, 672 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2190.

[Abstract] Bacteria use quorum-sensing (QS) systems to monitor and regulate their population density. Bacterial QS involves small molecules that act as signals for bacterial communication. Many Gram-negative bacterial pathogens use a class of widely conserved molecules, called diffusible signal factor (DSF) family ...

Isolation of Outer Membrane Vesicles from Phytopathogenic Xanthomonas campestris pv. campestris

Authors: Gideon Mordukhovich
Gideon MordukhovichAffiliation 1: Department of Plant Pathology and Weed Research, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
Affiliation 2: Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot, Israel
For correspondence: gideon.mordukhovic@mail.huji.ac.il
Bio-protocol author page: a4144
 and Ofir Bahar
Ofir BaharAffiliation: Department of Plant Pathology and Weed Research, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel
For correspondence: ofirb@agri.gov.il
Bio-protocol author page: a4145
,  date: 3/5/2017, 648 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2160.

[Abstract] Gram-negative bacteria naturally release outer membrane vesicles (OMVs) to the surrounding environment. OMVs contribute to multiple processes, such as cell-cell communication, delivery of enzymes and toxins, resistance to environmental stresses and pathogenesis. Little is known about OMVs produced by ...
1 
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