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Coomassie Blue Staining

Fanglian He Fanglian He

Published: Jun 5, 2011 DOI: 10.21769/BioProtoc.78 Views: 82264

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Abstract

Coomassie staining is able to detect protein bands containing about 0.2 μg or more protein. For low abundant protein, silver staining (www/silver staining) is a better choice since it is about 100-fold more sensitive than Coomassie staining.

Keywords: Coomassie staining search Coomassie blue search Staining solution search Destaining solution search Protein search

Background

Materials and Reagents

  1. Coomassie Brilliant Blue R250 (EM Science)
  2. Glacial acetic acid
  3. MetOH
  4. Staining solution
  5. Dye solution
  6. Destaining solution

Equipment

  1. Shaker

Procedure

Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.

Category

Biochemistry > Protein > Electrophoresis

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