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Published: May 20, 2011 DOI: 10.21769/BioProtoc.73 Views: 19613
Abstract
This method uses a PEG-supplemented liquid solution to germinate separated Aradidopsis pollen. It thus eliminated the need for humidity control.
Keywords: Pollen tubeBackground
Materials and Reagents
| Stock | Final Conc. | V stock/40 ml | |
| MES-Tris (pH 5.8 adjusted with Tris base) | 200 mM | 5 mM | 1 ml |
| KCl | 1 M | 1 mM | 40 μl |
| MgSO4 | 0.5 M | 0.8 mM | 64 μl |
| Boric acid | 100 mM | 1.5 mM | 600 μl |
| CaCl2 | 0.5 M | 10 mM | 800 μl |
| Sucrose | 5% w/v | 2 g | |
| PEG4000 | 15% w/v | 6 g |
Equipment
Procedure
Category
Plant Science > Plant physiology > Tissue analysis
Cell Biology > Tissue analysis > Tissue isolation
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