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Single Worm PCR
Published: Apr 20, 2011 DOI: 10.21769/BioProtoc.60 Views: 20863
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Abstract
This protocol is used for genotyping single worms.
Materials and Reagents
- Nonidet P-40 (Sigma-Aldrich, catalog number: 74385 )
- Gelatin (Sigma-Aldrich)
- Proteinase K (Promega Corporation, catalog number: V3021 )
- KCl
- Tris (pH 8.3)
- MgCl2
- Nonidet P-40
- Tween-20
- Gelatinin
- Lysis buffer (see Recipes)
Equipment
- PCR tubes
Procedure
Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.
Category
Molecular Biology > DNA > PCR
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