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In utero Electroporation of Mouse Cerebellar Purkinje Cells   

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Original research article

A brief version of this protocol appeared in:
The Journal of Neuroscience
Sep 2015

Abstract

In utero electroporation (IUE) of mouse cerebellar Purkinje cells allows high expression levels of transgenes without toxicity (Nishiyama et al., 2012). This technique is suitable for co-transfection of multiple plasmid genes. Therefore, it is useful to express various sets of genes such as drug-inducible Cre/loxP constructs and CRISPR/Cas9 genome editing constructs (Takeo et al., 2015). Murine Purkinje cells arise from subventricular zone of fourth ventricle at embryonic day (E) 10-12. IUE at E11.5 into fourth ventricle results the most efficient transfection into Purkinje cells.

Keywords: Purkinje cell, In utero electroporation, Cerebellum

Copyright: © 2016 The Authors; exclusive licensee Bio-protocol LLC.
How to cite:  Readers should cite both the Bio-protocol article and the original research article where this protocol was used:
  1. Takeo, Y. H. (2016). In utero Electroporation of Mouse Cerebellar Purkinje Cells. Bio-protocol 6(11): e1835. DOI: 10.21769/BioProtoc.1835.
  2. Takeo, Y. H., Kakegawa, W., Miura, E. and Yuzaki, M. (2015). RORalpha regulates multiple aspects of dendrite development in cerebellar purkinje cells in vivo. J Neurosci 35(36): 12518-12534.
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