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Genotyping for Single Zebrafish (Fin Clip) or Zebrafish Embryo
Published: Feb 5, 2012 DOI: 10.21769/BioProtoc.182 Views: 28554
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Abstract
Zebrafish is increasingly used a genetic model organism in biomedical studies. This protocol provides a detailed procedure about the identification of the genotype of an adult zebrafish or a zebrafish embryo.
Materials and Reagents
- Tricane (Sigma-Aldrich, catalog number: 886-86-2 )
- dNTPS (QIAGEN, catalog number: 201900 )
- Proteinase K (Roche Diagnostics, catalog number: 03115836001 )
- MEOH
- DNA polymerase
- MgCl2
- Tris
- KCl
- MgCl2
- Gelatine
- BSA
- NP40
- Tween 20
- PCR lysis buffer (see Recipes)
- RAPD+ (see Recipes)
- 1x RAPD buffer (see Recipes)
- 2x RAPD+ buffer (see Recipes)
- Tricane solution (see Recipes)
Equipment
- Standard tabletop centrifuges
- Incubator (55 °C and 65 °C)
- PCR thermal cycler
- 96-well PCR plate
- Razors
- Plastic plates
- Plastic beaker
- Forceps
- Spoon
Procedure
Category
Molecular Biology > DNA > Genotyping
Molecular Biology > DNA > PCR
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