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Transformation of the Cyanobacterium Leptolyngbya boryana by Electroporation   

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Original research article

A brief version of this protocol appeared in:
Plant & Cell Physiology
Feb 2015

Abstract

Leptolyngbya boryana (L. boryana) (formerly Plectonema boryanum) is a versatile, filamentous cyanobacterium that has the ability to fix nitrogen under microoxic conditions and to grow heterotrophically with glucose in the dark, providing an excellent system to investigate photosynthesis, nitrogen fixation, and their regulatory mechanisms. While L. boryana is not naturally transformable different from the unicellular cyanobacterium Synechocystis sp. PCC 6803, it can be transformed by electroporation. Here we describe the transformation of L. boryana by electroporation to isolate mutants in which a targeted gene is disrupted.

Keywords: Cyanobacteria, Transformation, Electroporation, Targeted gene disruption, Shuttle vector

Copyright: © 2015 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Tsujimoto, R., Kotani, H., Nonaka, A., Miyahara, Y., Hiraide, Y. and Fujita, Y. (2015). Transformation of the Cyanobacterium Leptolyngbya boryana by Electroporation. Bio-protocol 5(24): e1690. DOI: 10.21769/BioProtoc.1690.
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