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Arabidopsis Growing Protocol – A General Guide
Published: Sep 5, 2011 DOI: 10.21769/BioProtoc.126 Views: 38139
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Abstract
Arabidopsis as the model organism for higher plants is widely studied among plant biology labs around the world. However, taking care of this tiny plant may not be trivial. Here is a general guide used for the Heven Sze lab at the University of Maryland, College Park. A lot of efforts have been taken by the Sze lab and fellow lab members to formulate a general guide for Arabidopsis plant growth in the lab.
Materials and Reagents
- Arabidopsis seed
- Miracle-Gro® Potting Mix with Fertilizer (2 cubic feet bag) (The Scotts Miracle-Gro Company, model: 74278300 from Lowe’s, Item: 156581)
- Miracle-Gro® 8 Qt. Miracle Gro® Perlite (The Scotts Miracle-Gro Company, model: 70752300 , Lowe’s, Item: 68468)
- 0.1% Triton X-100
- Deionized water (D.I. water)
- Sterile D.I. water
- 70% ethanol
- Drierite
- KNO3
- FeNa-EDTA
- KH2PO4
- MgSO4
- Ca(NO3)2.4H2O
- Sterilization solution (see Recipes)
- 1/4 Strength Hoagland’s solution (see Recipes)
- Homemade MS (Murashige and Skoog, 1962) medium for Arabidopsis plants under different Ca2+/Mn2+ conditions (see Recipes)
Equipment
- The Scotts Miracle-Gro Company
- Autoclave bin
- Scooper
- Fisher dishes
- Pots (14 x 8.5 x 6 cm)
- Aluminum foil
- Humidome
- Sealed container
- Nylon mesh
- Autoclave tape
- Growth chamber
- Microcentrifuge
- Small Fisher dishes (100 x 15 mm size)
- Whatman filter paper
Procedure
Category
Plant Science > Plant physiology > Plant growth
Plant Science > Plant developmental biology > General
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