Seed Coat Ruthenium Red Staining Assay   

Edited by
Ru Zhang
Reviewed by
Anonymous reviewer
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In this protocol

Original research article

A brief version of this protocol appeared in:
The Plant Cell
Jul 2013


The goal of this protocol is to assay for defects in synthesis/secretion/release of seed coat mucilage by ruthenium red staining of mature whole seeds.
The mucilage secretory cells of the Arabidopsis seed coat synthesize and secrete a large quantity of primarily pectinaceous mucilage to a ring-shaped apical domain during their differentiation. This makes them an excellent model system to identify genes involved in both cell wall synthesis and secretion (et al., 2000). When wild-type seeds are incubated in ruthenium red stain, hydrated mucilage is extruded from epidermal cells and a ‘halo’ of red-stained mucilage is observed surrounding the seed (Western et al., 2000). Reduced mucilage staining may result from defects in cell wall biosynthesis, secretion, or impaired release upon hydration.

Keywords: Arabidopsis, Seed coat, Mucilage, Ruthenium red, Pectin

Copyright: © 2014 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: McFarlane, H. E., Gendre, D. and Western, T. L. (2014). Seed Coat Ruthenium Red Staining Assay. Bio-protocol 4(7): e1096. DOI: 10.21769/BioProtoc.1096.

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