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DNA PCR Assays for Igh Rearrangement   

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In this protocol

Original research article

A brief version of this protocol appeared in:
Immunity
Jun 2013

Abstract

This protocol is used for the detection of immunoglobulin heavy (H) chain rearrangements. This PCR-based assay enables detection of DH-JH recombination in cultured hematopoietic cells (Schlissel et al., 1991; Satoh et al., 2013) [e.g. ES-derived cells (Satoh et al., 2013)].

Copyright: © 2014 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Satoh, Y., Sudo, T. and Yokota, T. (2014). DNA PCR Assays for Igh Rearrangement. Bio-protocol 4(4): e1046. DOI: 10.21769/BioProtoc.1046.
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