Editors' Choice Protocols

编辑推荐

Editors' Choice corresponds to recently published bio-protocols (past one year) that chosen by Bio-protocol Associate Editors based on the protocols' quality, novelty, or potential impact.

研究领域

生物化学
生物工程
生物科学
癌症生物学
细胞生物学
计算生物学与生物信息学
更正
发育生物学
药物发现
杂志社论
免疫学
力学生物学
医学
微生物学
分子生物学
神经科学
植物科学
产品文档
撤稿
干细胞
系统生物学
更新

研究物种

节肢动物
鱼类
真菌
无脊椎动物
哺乳纲
其它脊椎动物
植物
原核生物
原生动物
病毒
蠕虫

Peptide Feeding and Mechanical Wounding for Tomato Seedlings
番茄幼苗的肽饲喂和机械伤害

作者：Lei Wang, Yan Wang and Georg Felix，日期：03/20/2019，浏览量：4876，Q&A： 0

Plants need to respond appropriately to wounding and herbivorous insects. Peptide signals have been implicated in local and systemic induction of appropriate plant defense responses. To study these ...

Viral Chromosome Conformation Capture (V3C) Assays for Identifying Trans-interaction Sites between Lytic Viruses and the Cellular Genome
病毒染色体构象俘获技术用于裂解病毒和细胞基因组之间反向相互作用位点的检测

作者：Kinjal Majumder, Maria Boftsi and David J Pintel，日期：03/20/2019，浏览量：4011，Q&A： 0

The folding mechanisms of the mammalian genome package our genetic material into the nucleus, and in doing so, dictate its appropriate replication and expression. Chromosome conformation capture ...

Straight Channel Microfluidic Chips for the Study of Platelet Adhesion under Flow
直通道微流控芯片用于研究流动中的血小板黏附

作者：Alexander Dupuy, Lining Arnold Ju and Freda H Passam，日期：03/20/2019，浏览量：12127，Q&A： 0

Microfluidic devices have become an integral method of cardiovascular research as they enable the study of shear force in biological processes, such as platelet function and thrombus formation. ...

Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos
整体原位杂交检测mRNA并提取DNA检测斑马鱼胚胎的基因分型

作者：Rachna Narayanan and Andrew C. Oates，日期：03/20/2019，浏览量：12938，Q&A： 0

In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current ...

Differentiation of Human Induced Pluripotent Stem Cells (iPSCs) into an Effective Model of Forebrain Neural Progenitor Cells and Mature Neurons
人诱导多能干细胞 (iPSCs) 分化为前脑神经祖细胞和成熟神经元的有效模型

作者：Scott Bell, Nuwan C. Hettige, Heika Silveira, Huashan Peng, Hanrong Wu, Malvin Jefri, Lilit Antonyan, Ying Zhang, Xin Zhang and Carl Ernst，日期：03/05/2019，浏览量：14375，Q&A： 0

Induced Pluripotent Stem Cells (iPSCs) are pluripotent stem cells that can be generated from somatic cells, and provide a way to model the development of neural tissues in vitro. One ...

Isolation of Neural Stem Cells from the Embryonic Mouse Hippocampus for in vitro Growth or Engraftment into a Host Tissue
从胚胎小鼠海马分离神经干细胞用于体外生长或植入宿主组织

作者：Oksana Rybachuk, Olga Kopach, Tetyana Pivneva and Vitaliy Kyryk，日期：02/20/2019，浏览量：7888，Q&A： 0

For both stem cell research and treatment of the central nervous system disorders, neural stem/progenitor cells (NSPCs) represent an important breakthrough tool. In the expanded stem cell-based ...

Imaging Higher-order Chromatin Structures in Single Cells Using Stochastic Optical Reconstruction Microscopy
随机光学重建显微镜用于单细胞中高级染色体结构的成像

作者：Jianquan Xu and Yang Liu，日期：02/05/2019，浏览量：6398，Q&A： 0

Higher-order chromatin organization shaped by epigenetic modifications influence the chromatin environment and subsequently regulate gene expression. Direct visualization of the higher-order ...

In situ, Cell-free Protein Expression on Microarrays and Their Use for the Detection of Immune Responses
基于原位无细胞蛋白表达的蛋白质微阵列技术及其在免疫应答检测中的应用

作者：Katrin Hufnagel, Dennis Reininger, Siu Wang Ng, Nadine Gassert, Juliane K. Rohland, Soroosh Shahryarhesami, Andrea S. Bauer, Tim Waterboer and Jörg D. Hoheisel，日期：02/05/2019，浏览量：5044，Q&A： 0

Until recently, whole-proteome microarrays for comprehensive studies of protein interactions were mostly produced by individual cloning and cellular expression of very many open reading frames, ...

Measuring Homologous Recombination Rates between Chromosomal Locations in Salmonella
沙门氏菌中染色体位点间的同源重组率测定

作者：Gerrit Brandis, Sha Cao and Diarmaid Hughes，日期：02/05/2019，浏览量：6568，Q&A： 0

Homologous recombination between two similar DNA molecules, plays an important role in the repair of double-stranded DNA breaks. Recombination can occur between two sister chromosomes, or between two ...

CRISPR-Cas9 Mediated Genome Editing in Drosophila
果蝇中CRISPR-Cas9介导的基因组编辑

作者：Ping Peng, Xia Wang, Da Shen, Jin Sun, Yu Jia, Rong-Gang Xu, Li-Fei Zhu and Jian-Quan Ni ，日期：01/20/2019，浏览量：9276，Q&A： 0

In recent years, great progress has been made in the research of genome editing systems, one of which is the CRISPR-Cas9 system, a powerful technology that is applied to edit animal genome. Here, we ...